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Mouse S100A8 Antibody

Catalog #: AF3059
26 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3059
AF3059-SP
Detection of Mouse S100A8 by Western Blot.
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Product Details
Citations (26)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse S100A8 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse S100A8 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant mouse (rm) S100A9, rmS100A10, and recombinant human S100B is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse S100A8
Pro2-Glu89
Accession # P27005
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.25 µg/mL
See below
Simple Western
2.5 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse S100A8 antibody by Western Blot. View Larger

Detection of Mouse S100A8 by Western Blot. Western blot shows lysates of mouse spleen tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse S100A8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3059) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for S100A8 at approximately 10-11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry S100A8 antibody in Mouse Splenocytes by Immunocytochemistry (ICC). View Larger

S100A8 in Mouse Splenocytes. S100A8 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse S100A8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3059) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunocytochemistry S100A8 antibody in NMuMG Mouse Cell Line by Immunocytochemistry (ICC). View Larger

S100A8 in NMuMG Mouse Cell Line. S100A8 was detected in immersion fixed NMuMG mouse mammary gland epithelial cell line using 10 µg/mL Goat Anti-Mouse S100A8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3059) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western Detection of Mouse S100A8 antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Mouse S100A8 by Simple WesternTM. Simple Western lane view shows lysates of mouse spleen tissue, loaded at 0.2 mg/mL. A specific band was detected for S100A8 at approximately 9 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Mouse S100A8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3059) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: S100A8

Mouse S100A8, also known as CP-10, Calgranulin A and MRP8, is a 10 kDa member of the S100 family of calcium-binding proteins. S100A8 contains short sequential modules, including an N-terminal alpha -helix, a Ca++-binding EF-hand segment, a short central linker region, a second EF-hand segment, and a C-terminal alpha -helix. S100A8 noncovalently heterodimerizes with S100A9. In the presence of Ca++, the heterodimers form heterotetramers. The dimeric complex is found both intracellularly and extracellularly. It binds to heparan sulfate and is chemotactic for PMNs and macrophages. The amino acid sequence of mouse S100A8 is 80% and 57% identical to that of rat and human S100A8, respectively.

Long Name
S100 Calcium Binding Protein A8
Entrez Gene IDs
6279 (Human); 20201 (Mouse)
Alternate Names
60B8AG; CAGACP-10; Calgranulin A; calgranulin-A; Calprotectin L1L subunit; CFAG; CFAGL1Ag; CGLA; Cystic fibrosis antigen; Leukocyte L1 complex light chain; MA387; MIF; Migration inhibitory factor-related protein 8; MRP8; MRP-8; MRP8S100 calcium binding protein A8 (calgranulin A); NIF; P8; protein S100-A8; S100 calcium binding protein A8; S100 calcium-binding protein A8 (calgranulin A); S100 calcium-binding protein A8; S100A8; Urinary stone protein band A

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Citations for Mouse S100A8 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

26 Citations: Showing 1 - 10
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  1. SRSF2 mutation reduces polycythemia and impairs hematopoietic progenitor functions in JAK2V617F-driven myeloproliferative neoplasm
    Authors: Yang, Y;Abbas, S;Sayem, MA;Dutta, A;Mohi, G;
    Blood cancer journal
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Bacterial meningitis in the early postnatal mouse studied at single-cell resolution
    Authors: Wang J, Rattner A, Nathans J
    eLife
  3. Neutrophil-derived interleukin-17A participates in neuroinflammation induced by traumatic brain injury
    Authors: Xu XJ, Ge QQ, Yang MS et al.
    Neural regeneration research
  4. ADAM8 signaling drives neutrophil migration and ARDS severity
    Authors: C Conrad, D Yildiz, SJ Cleary, A Margraf, L Cook, U Schlomann, B Panaretou, JL Bowser, H Karmouty-Q, J Li, NK Berg, SC Martin, A Aljohmani, SF Moussavi-H, KM Wang, JJ Tian, M Magnen, C Valet, L Qiu, JP Singer, HK Eltzschig, CAPSys Stu, W Bertrams, S Herold, N Suttorp, B Schmeck, ZT Ball, A Zarbock, MR Looney, JW Bartsch
    JCI Insight, 2022-02-08;7(3):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Single cell transcriptomic landscape of diabetic foot ulcers
    Authors: G Theocharid, BE Thomas, D Sarkar, HL Mumme, WJR Pilcher, B Dwivedi, T Sandoval-S, RF Sîrbulescu, A Kafanas, I Mezghani, P Wang, A Lobao, IS Vlachos, B Dash, HC Hsia, V Horsley, SS Bhasin, A Veves, M Bhasin
    Nature Communications, 2022-01-10;13(1):181.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  6. Longitudinal single cell transcriptomics reveals a role for Serpina3n-mediated resolution of inflammation in a mouse colitis model
    Authors: YT Ho, T Shimbo, E Wijaya, T Kitayama, S Takaki, K Ikegami, K Miyashita, Y Ouchi, E Takaki, R Yamamoto, Y Kaneda, K Tamai
    Cellular and Molecular Gastroenterology and Hepatology, 2021-04-13;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. A CD209 ligand and a sialidase inhibitor differentially modulate adipose tissue and liver macrophage populations and steatosis in mice on the Methionine and Choline-Deficient (MCD) diet
    Authors: Darrell Pilling, Tejas R. Karhadkar, Richard H. Gomer
    PLOS ONE
  8. Reduced Fecal Calprotectin and Inflammation in a Murine Model of Atopic Dermatitis Following Probiotic Treatment
    Authors: MJ Kim, JY Kim, M Kang, MH Won, SH Hong, Y Her
    Int J Mol Sci, 2020-05-31;21(11):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. RNA sequencing for ligature induced periodontitis in mice revealed important role of S100A8 and S100A9 for periodontal destruction
    Authors: S Maekawa, S Onizuka, S Katagiri, M Hatasa, Y Ohsugi, N Sasaki, K Watanabe, A Ohtsu, R Komazaki, K Ogura, T Miyoshi-Ak, T Iwata, H Nitta, Y Izumi
    Sci Rep, 2019-10-11;9(1):14663.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  10. Hypoxia-inducible factor-1 (HIF-1) activation in myeloid cells accelerates DSS-induced colitis progression in mice
    Authors: YE Kim, M Lee, H Gu, J Kim, S Jeong, S Yeo, YJ Lee, SH Im, YC Sung, HJ Kim, IL Weissman, GO Ahn
    Dis Model Mech, 2018-07-30;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  11. C-terminal truncation of IFN-? inhibits proinflammatory macrophage responses and is deficient in autoimmune disease
    Authors: A Dufour, CL Bellac, U Eckhard, N Solis, T Klein, R Kappelhoff, N Fortelny, P Jobin, J Rozmus, J Mark, P Pavlidis, V Dive, SJ Barbour, CM Overall
    Nat Commun, 2018-06-20;9(1):2416.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  12. Exploring experimental cerebral malaria pathogenesis through the characterisation of host-derived plasma microparticle protein content
    Sci Rep, 2016-12-05;6(0):37871.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  13. Beneficial Effects of Sodium Phenylbutyrate Administration During Infection with Salmonella enterica serovar Typhimurium
    Infect Immun, 2016-08-19;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  14. Zinc and Manganese Chelation by Neutrophil S100A8/A9 (Calprotectin) Limits Extracellular Aspergillus fumigatus Hyphal Growth and Corneal Infection
    Authors: Heather L. Clark, Anupam Jhingran, Yan Sun, Chairut Vareechon, Steven de Jesus de Jesus Carrion, Eric P. Skaar et al.
    The Journal of Immunology
  15. Loss of JUNB/AP-1 promotes invasive prostate cancer.
    Authors: Thomsen M, Bakiri L, Hasenfuss S, Wu H, Morente M, Wagner E
    Cell Death Differ, 2014-12-19;22(4):574-82.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  16. The junctional epithelium originates from the odontogenic epithelium of an erupted tooth.
    Authors: Yajima-Himuro, Sara, Oshima, Masamits, Yamamoto, Gou, Ogawa, Miho, Furuya, Madoka, Tanaka, Junichi, Nishii, Kousuke, Mishima, Kenji, Tachikawa, Tetsuhik, Tsuji, Takashi, Yamamoto, Matsuo
    Sci Rep, 2014-05-02;4(0):4867.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  17. Neutrophil dependence of vascular remodeling after Mycoplasma infection of mouse airways.
    Authors: Baluk P, Phillips K, Yao L, Adams A, Nitschke M, McDonald D
    Am J Pathol, 2014-04-13;184(6):1877-89.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  18. Preferential lymphatic growth in bronchus-associated lymphoid tissue in sustained lung inflammation.
    Authors: Baluk P, Adams A, Phillips K, Feng J, Hong Y, Brown M, McDonald D
    Am J Pathol, 2014-03-11;184(5):1577-92.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  19. S100A9 knockout decreases the memory impairment and neuropathology in crossbreed mice of Tg2576 and S100A9 knockout mice model.
    Authors: Kim H, Chang K, Ha T, Kim J, Ha S, Shin K, Moon C, Nacken W, Kim H, Suh Y
    PLoS ONE, 2014-02-25;9(2):e88924.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  20. The cytokine IL-22 promotes pathogen colonization by suppressing related commensal bacteria.
    Authors: Behnsen J, Jellbauer S, Wong C, Edwards R, George M, Ouyang W, Raffatellu M
    Immunity, 2014-02-06;40(2):262-73.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  21. Vaginal epithelial cell-derived S100 alarmins induced by Candida albicans via pattern recognition receptor interactions are sufficient but not necessary for the acute neutrophil response during experimental vaginal candidiasis.
    Authors: Yano J, Palmer G, Eberle K, Peters B, Vogl T, McKenzie A, Fidel P
    2013-12-09;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  22. Tumor necrosis factor-alpha blocks differentiation and enhances suppressive activity of immature myeloid cells during chronic inflammation.
    Authors: Sade-Feldman M, Kanterman J, Ish-Shalom E, Elnekave M, Horwitz E, Baniyash M
    Immunity, 2013-03-07;38(3):541-54.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: Neutralization, Western Blot
  23. The Acute Neutrophil Response Mediated by S100 Alarmins during Vaginal Candida Infections Is Independent of the Th17-Pathway.
    Authors: Yano, Junko, Kolls, Jay K, Happel, Kyle I, Wormley, Floyd, Wozniak, Karen L, Fidel, Paul L J
    PLoS ONE, 2012-09-25;7(9):e46311.
    Species: Mouse
    Sample Types: Vaginal Lavage Fluid
    Applications: ELISA Development
  24. MMP-8 Deficiency Increases TLR/RAGE Ligands S100A8 and S100A9 and Exacerbates Lung Inflammation during Endotoxemia.
    Authors: Gonzalez-Lopez A, Aguirre A, Lopez-Alonso I, Amado L, Astudillo A, Fernandez-Garcia MS, Suarez MF, Batalla-Solis E, Colado E, Albaiceta GM
    PLoS ONE, 2012-06-29;7(6):e39940.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  25. Biomarkers of Therapeutic Response in the IL-23 Pathway in Inflammatory Bowel Disease
    Authors: Corinne Cayatte, Barbara Joyce-Shaikh, Felix Vega, Katia Boniface, Jeffrey Grein, Erin Murphy et al.
    Clinical and Translational Gastroenterology
  26. Myeloid-Derived Suppressor Cells Infiltrate the Heart in Acute Trypanosoma cruzi Infection.
    Authors: Cuervo H, Guerrero NA, Carbajosa S, Beschin A, De Baetselier P, Girones N, Fresno M
    J. Immunol., 2011-07-29;187(5):2656-65.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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