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Mouse S1P1/EDG-1 Biotinylated Antibody

Catalog #: FAB7089B Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB7089B
Detection of S1P1/EDG‑1 in HEK293 Human Cell Line Transfected with Mouse S1P1/EDG-1 and eGFP by Flow Cytometry.
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Mouse S1P1/EDG-1 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse S1P1/EDG‑1 peptide in direct ELISAs.
Source
Monoclonal Rat IgG2A Clone # 713412
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse S1P1/EDG‑1 synthetic peptide (T4-H28)
Accession # O08530
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Biotin

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of S1P<SUB>1</SUB>/EDG-1 antibody in HEK293 Human Cell Line Transfected with Mouse S1P<SUB>1</SUB>/EDG-1 and eGFP antibody by Flow Cytometry. View Larger

Detection of S1P1/EDG‑1 in HEK293 Human Cell Line Transfected with Mouse S1P1/EDG-1 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with mouse S1P1/EDG-1 and eGFP was stained with (A) Rat Anti-Mouse S1P1/EDG-1 Biotinylated Monoclonal Antibody (Catalog # FAB7089B) or (B) Rat IgG2A isotype control antibody (Catalog # IC006B) followed by Streptavidin-Allophycocyanin (Catalog # F0050). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of S1P<SUB>1</SUB>/EDG-1 antibody in Mouse thymocytes antibody by Flow Cytometry. View Larger

Detection of S1P1/EDG‑1 in Mouse thymocytes by Flow Cytometry. Mouse thymocytes were stained with (A) Rat Anti-Mouse S1P1/EDG-1 Biotinylated Monoclonal Antibody (Catalog # FAB7089B) or (B) Rat IgG2A isotype control antibody (Catalog # IC006B) followed by Streptavidin-Allophycocyanin (Catalog # F0050) and Rat Anti-Mouse CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB554P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: S1P1/EDG-1

S1P1 (sphingosine 1-phosphate receptor-1), also known as EDG-1 (endothelial differentiation, G-protein coupled receptor-1) or S1PR1 (sphingosine-1-phosphate receptor 1) is a widely expressed, 37-40 kDa, G protein coupled receptor within the S1P subfamily of the EDG family. S1P1 is one of five receptors for the bioactive lipid S1P and mediates most of S1P effects on angiogenesis, vascular maturation, and cell migration, especially T cell egress from lymphoid organs. Human and mouse S1P1 share 84% amino acid identity within the N-terminal extracellular portion used as an immunogen.

Long Name
Endothelial Differentiation Gene 1
Entrez Gene IDs
1901 (Human); 13609 (Mouse)
Alternate Names
CD363 antigen; CD363; CHEDG1; D1S3362; EDG1; EDG-1; EDG1edg-1; Endothelial differentiation G-protein coupled receptor 1; endothelial differentiation, sphingolipid G-protein-coupled receptor, 1; FLJ58121; S1P receptor 1; S1P receptor Edg-1; S1P1; S1P1ECGF1; S1PR1; sphingosine 1-phosphate receptor 1; sphingosine 1-phosphate receptor EDG1; Sphingosine 1-phosphate receptor Edg-1; sphingosine-1-phosphate receptor 1

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FAQs

  1. Can staining with Mouse S1P1/EDG-1 conjugated Antibody, catalog # FAB7089*,  be carried out at 4°C? 

    • There is evidence that G protein family proteins require staining at room temperature (RT) or even 37 °C, so this is what we would recommend.  Staining for 45 minutes is optimal with this antibody. 

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