Home / Serpin A1a / Mouse Serpin A1a Antibody

Mouse Serpin A1a Antibody

Catalog #: MAB7690
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB7690
MAB7690-SP
Detection of Mouse SerpinA1a by Western Blot.
2 Images
Product Details
Citations (1)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Serpin A1a Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Serpin A1a in ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant mouse (rm) Serpin A1c, approximately 15% cross-reactivity with recombinant human A1, and less than 5% cross-reactivity with rmSerpin A3n is observed.
Source
Monoclonal Rat IgG1 Clone # 882213
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Serpin A1a
Met1-Lys413
Accession # P07758
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below
Simple Western
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse SerpinA1a antibody by Western Blot. View Larger

Detection of Mouse SerpinA1a by Western Blot. Western blot shows mouse serum. PVDF membrane was probed with 0.2 µg/mL of Rat Anti-Mouse Serpin A1a Monoclonal Antibody (Catalog # MAB7690) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Serpin A1a at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Mouse Serpin A1a antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Mouse Serpin A1a by Simple WesternTM. Simple Western lane view shows mouse serum, loaded at 0.2 mg/mL. A specific band was detected for Serpin A1a at approximately 62 kDa (as indicated) using 2 µg/mL of Rat Anti-Mouse Serpin A1a Monoclonal Antibody (Catalog # MAB7690) followed by 1:50 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin A1a

Serpin A1a, also known as alpha-1-antitrypsin, belongs to the serpin superfamily of proteinase inhibitors. It is one of five alpha 1-proteinase inhibitors produced in mice. Serpin A1a is produced in the liver, then secreted into the bloodstream. It inhibits a number of serine proteases, including elastase, trypsin, plasmin, and thrombin (1, 2). Serpin A1a’s major function is to protect the lungs from neutrophil elastase. Serpin A1a deficiency can cause complications such as emphysema, and chronic obstructive pulmonary disease (COPD).

References
  1. Gettins, P.G. (2002) Chem. Rev. 102:4751.
  2. Kalsheker N.A. (1989) Biosci. Rep. 9:129. 3. Ritchie, R.F. et al. (2000) J. Clin. Lab. Anal. 14:284.
Entrez Gene IDs
5265 (Human); 20700 (Mouse)
Alternate Names
A1A; A1AT; AAT; Aat2; alpha1AT; Dom1; nNIF; PI; PI1; PRO2275; Serpin A1a; Spi1-1

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Citation for Mouse Serpin A1a Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Rosa26-LSL-dCas9-VPR: a versatile mouse model for tissue specific and simultaneous activation of multiple genes for drug discovery
    Authors: D Pakalnišky, T Schönberge, B Strobel, B Stierstorf, T Lamla, M Schuler, M Lenter
    Scientific Reports, 2022-11-10;12(1):19268.
    Species: Transgenic Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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