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Mouse SPARC Antibody

Catalog #: AF942
25 Citations 3 Reviews Datasheet / COA / SDS
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AF942
AF942-SP
Detection of Mouse SPARC by Western Blot.
4 Images
Product Details
Citations (25)
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Supplemental Products
Reviews (3)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse SPARC Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse SPARC in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse SPARC/Osteonectin
Ala18-Ile302
Accession # P07214
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below
Immunohistochemistry
1-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
See below
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
Balb/C-3T3 mouse embryonic fibroblast cell line fixed with paraformaldehyde and permeabilized with saponin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse SPARC antibody by Western Blot. View Larger

Detection of Mouse SPARC by Western Blot. Western blot shows lysates of C2C12 mouse myoblast cell line and mouse placenta tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse SPARC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF942) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for SPARC at approximately 35-37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry SPARC/Osteonectin antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

SPARC/Osteonectin in Mouse Embryo. SPARC/Osteonectin was detected in immersion fixed frozen sections of mouse embryo (E15) using Mouse SPARC/ Osteonectin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF942) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to developing cartilage. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Immunocytochemistry SPARC antibody in C2C12 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

SPARC in C2C12 Mouse Cell Line. SPARC was detected in immersion fixed C2C12 mouse myoblast cell line using Goat Anti-Mouse SPARC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF942) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to endoplasmic reticuli. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Intracellular Staining by Flow Cytometry View Larger

Detection of SPARC in Balb/C-3T3 Mouse Cell Line by Flow Cytometry. Balb/C-3T3 mouse fibroblast cell line was stained with Goat Anti-Mouse SPARC Polyclonal Antibody (Catalog # AF942, filled histogram) or Goat IgG control antibody (AB-108-C, open histogram), followed by Phycoerythrin-conjugated anti-Goat IgG (F0107). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SPARC

SPARC, an acronym for “secreted protein, acidic and rich in cysteine”, is also known as osteonectin or BM-40 (1-5). It is the founding member of a family of secreted matricellular proteins with similar domain structure. The 302 amino acid (aa), 43 kDa protein contains a 17 aa signal sequence, an N-terminal acidic region that binds calcium, a follistatin domain containing Kazal-like sequences, and a C-terminal extracellular calcium (EC) binding domain with two EF-hand motifs (1-5). Crystal structure shows that residues implicated in cell binding, inhibition of cell spreading and disassembly of focal adhesions cluster on one face of SPARC, while a collagen binding epitope and an N-glycosylation site are opposite this face (6). SPARC is produced by fibroblasts, capillary endothelial cells, platelets, and macrophages, especially in areas of tissue morphogenesis and remodeling (3, 7). SPARC shows context-specific effects, but generally inhibits adhesion, spreading and proliferation, and promotes collagen matrix formation (3-5). For endothelial cells, SPARC disrupts focal adhesions and binds and sequesters PDGF and VEGF (3-5). SPARC is abundantly expressed in bone, where it promotes osteoblast differentiation and inhibits adipogenesis (5, 8). SPARC is potentially cleaved by metalloproteinases, producing an angiogenic peptide that includes the copper-binding sequence KGHK (7). Paradoxically, SPARC is highly expressed in many tumor types, yet expression mainly decreases the likelihood of metastasis and confers sensitivity to chemotherapy and radiation (4, 9, 10). Stabilin-1, which is expressed on alternately activated macrophages, is the first SPARC receptor to be identified. It binds the SPARC EC domain and mediates endocytosis for degradation (11). Mature mouse SPARC shows 97%, 92%, 92%, 92%, and 83% aa identity with rat, human, dog, cow, and chick SPARC, respectively.

References
  1. Lankat-Buttgereit, B. et al. (1988) FEBS Lett. 236:352.
  2. McVey, J.H. et al. (1988) J. Biol. Chem. 263:11111.
  3. Sage, H. et al. (1989) J. Cell Biol. 109:341.
  4. Framson, P.E. and E.H. Sage (2004) J. Cell. Biochem. 92:679.
  5. Alford, A.I. and K.D. Hankenson (2006) Bone 38:749.
  6. Hohenester, E. et al. (1997) EMBO J. 16:3778.
  7. Sage, E.H. et al. (2003) J. Biol. Chem. 278:37849.
  8. Delany, A.M. et al. (2003) Endocrinology 144:2588.
  9. Koblinski, J.E. et al. (2005) Cancer Res. 65:7370.
  10. Tai, I.T. et al. (2005) J. Clin. Invest. 115:1492.
  11. Kzhyshkowska, J. et al. (2006) J. Immunol. 176:5825.
Long Name
Secreted Protein Acidic and Rich in Cysteine
Entrez Gene IDs
6678 (Human); 20692 (Mouse)
Alternate Names
Basement-membrane protein 40; BM-40; ONcysteine-rich protein; Osteonectin; Secreted protein acidic and rich in cysteine; secreted protein, acidic, cysteine-rich (osteonectin); SPARC

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Citations for Mouse SPARC Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

25 Citations: Showing 1 - 10
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  1. Astrocyte modulation of synaptic plasticity mediated by activity-dependent Sonic hedgehog signaling
    Authors: Le, AD;Fu, M;Kumar, R;Zacharias, G;Garcia, ADR;
    bioRxiv : the preprint server for biology
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  2. Abnormal Morphology and Synaptogenic Signaling in Astrocytes Following Prenatal Opioid Exposure
    Authors: Niebergall, EB;Weekley, D;Mazur, A;Olszewski, NA;DeSchepper, KM;Radant, N;Vijay, AS;Risher, WC;
    Cells
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Vitamin D Receptor Expression Limits the Angiogenic and Inflammatory Properties of Retinal Endothelial Cells
    Authors: YS Song, N Jamali, CM Sorenson, N Sheibani
    Cells, 2023-01-16;12(2):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Effect of SPARC Suppression in Mice, Perfused Human Anterior Segments, and Trabecular Meshwork Cells
    Authors: WW MacDonald, SS Swaminatha, JY Heo, A Castillejo, J Hsueh, BJ Liu, D Jo, A Du, H Lee, MH Kang, DJ Rhee
    Investigative Ophthalmology & Visual Science, 2022-06-01;63(6):8.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Astrocyte-neuron crosstalk through Hedgehog signaling mediates cortical synapse development
    Authors: Y Xie, AT Kuan, W Wang, ZT Herbert, O Mosto, O Olukoya, M Adam, S Vu, M Kim, D Tran, N Gómez, C Charpentie, I Sorour, TE Lacey, MY Tolstoruko, BL Sabatini, WA Lee, CC Harwell
    Cell Reports, 2022-02-22;38(8):110416.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Secreted Protein Acidic and Rich in Cysteine (Sparc) KO Leads to an Accelerated Ageing Phenotype Which Is Improved by Exercise Whereas SPARC Overexpression Mimics Exercise Effects in Mice
    Authors: A Ghanemi, A Melouane, M Yoshioka, J St-Amand
    Metabolites, 2022-01-28;12(2):.
    Species: Mouse
    Sample Types: Protein
    Applications: Western Blot
  7. USP22 Suppresses SPARC Expression in Acute Colitis and Inflammation-Associated Colorectal Cancer
    Authors: RL Kosinsky, D Saul, C Ammer-Herr, WA Faubion, A Neesse, SA Johnsen
    Cancers, 2021-04-10;13(8):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. Differential Proliferation and Maturation of Subcortical Astrocytes During Postnatal Development
    Authors: T Shoneye, AT Orrego, R Jarvis, Y Men, MSR Chiang, Y Yang
    Front Neurosci, 2020-05-08;14(0):435.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. Bim expression modulates the pro-inflammatory phenotype of retinal astroglial cells
    Authors: J Falero-Per, N Sheibani, CM Sorenson
    PLoS ONE, 2020-05-04;15(5):e0232779.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Tracing tumorigenesis in a solid tumor model at single-cell resolution
    Authors: SD Praktiknjo, B Obermayer, Q Zhu, L Fang, H Liu, H Quinn, M Stoeckius, C Kocks, W Birchmeier, N Rajewsky
    Nat Commun, 2020-02-20;11(1):991.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. SPARC dependent collagen deposition and gemcitabine delivery in a genetically engineered mouse model of pancreas cancer
    Authors: I Ramu, SM Buchholz, MS Patzak, RG Goetze, SK Singh, FM Richards, DI Jodrell, B Sipos, P Ströbel, V Ellenriede, E Hessmann, A Neesse
    EBioMedicine, 2019-10-06;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC-P, Western Blot
  12. Extracellular SPARC increases cardiomyocyte contraction during health and disease
    Authors: S Deckx, DM Johnson, M Rienks, P Carai, E Van Deel, J Van der Ve, KR Sipido, S Heymans, AP Papageorgi
    PLoS ONE, 2019-04-01;14(4):e0209534.
    Species: Mouse
    Sample Types: Cell Fraction, Whole Cells
    Applications: ICC, Western Blot
  13. Cyp1b1 expression impacts the angiogenic and inflammatory properties of liver sinusoidal endothelial cells
    Authors: J Falero-Per, YS Song, Y Zhao, L Teixeira, CM Sorenson, N Sheibani
    PLoS ONE, 2018-10-29;13(10):e0206756.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  14. SPARC and GluA1-Containing AMPA Receptors Promote Neuronal Health Following CNS Injury
    Authors: EV Jones, Y Bernardine, JG Zarruk, S Chierzi, KK Murai
    Front Cell Neurosci, 2018-02-01;12(0):22.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-P, Western Blot
  15. Molecular and Functional Properties of Regional Astrocytes in the Adult Brain
    Authors: L Morel, MSR Chiang, H Higashimor, T Shoneye, LK Iyer, J Yelick, A Tai, Y Yang
    J. Neurosci., 2017-08-07;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  16. Stabilin-1 is expressed in human breast cancer and supports tumor growth in mammary adenocarcinoma mouse model
    Oncotarget, 2016-05-24;7(21):31097-110.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC-Fr, Western Blot
  17. Proteolytic Isoforms of SPARC Induce Adipose Stromal Cell Mobilization in Obesity.
    Authors: Tseng C, Kolonin M
    Stem Cells, 2015-09-22;34(1):174-90.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-P, Western Blot
  18. CSF1-ETS2-induced microRNA in myeloid cells promote metastatic tumor growth.
    Authors: Mathsyaraja H, Thies K, Taffany D, Deighan C, Liu T, Yu L, Fernandez S, Shapiro C, Otero J, Timmers C, Lustberg M, Chalmers J, Leone G, Ostrowski M
    Oncogene, 2014-09-22;34(28):3651-61.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  19. Stromal niche communalities underscore the contribution of the matricellular protein SPARC to B-cell development and lymphoid malignancies.
    Authors: Sangaletti, Sabina, Tripodo, Claudio, Portararo, Paola, Dugo, Matteo, Vitali, Caterina, Botti, Laura, Guarnotta, Carla, Cappetti, Barbara, Gulino, Alessand, Torselli, Ilaria, Casalini, Patrizia, Chiodoni, Claudia, Colombo, Mario P
    Oncoimmunology, 2014-06-05;3(0):e28989.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC, IHC-P
  20. Losartan slows pancreatic tumor progression and extends survival of SPARC-null mice by abrogating aberrant TGF beta activation.
    Authors: Arnold SA, Rivera LB, Carbon JG, Toombs JE, Chang CL, Bradshaw AD, Brekken RA
    PLoS ONE, 2012-02-14;7(2):e31384.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  21. Astrocytes control glutamate receptor levels at developing synapses through SPARC-beta-integrin interactions.
    Authors: Jones EV, Bernardinelli Y, Tse YC, Chierzi S, Wong TP, Murai KK
    J. Neurosci., 2011-03-16;31(11):4154-65.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  22. Absence of SPARC results in increased cardiac rupture and dysfunction after acute myocardial infarction.
    Authors: Schellings MW, Vanhoutte D, Swinnen M, Cleutjens JP, Debets J, van Leeuwen RE, d'Hooge J, Van de Werf F, Carmeliet P, Pinto YM, Sage EH, Heymans S
    J. Exp. Med., 2008-12-22;206(1):113-23.
    Species: Mouse, Rat
    Sample Types: Cell Lysates, Tissue Homogenates
    Applications: Western Blot
  23. A conserved organization of transcription during embryonic stem cell differentiation and in cells with high C value.
    Authors: Faro-Trindade I, Cook PR
    Mol. Biol. Cell, 2006-04-19;17(7):2910-20.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  24. Loss of SPARC-mediated VEGFR-1 suppression after injury reveals a novel antiangiogenic activity of VEGF-A.
    Authors: Nozaki M, Sakurai E, Raisler BJ, Baffi JZ, Witta J, Ogura Y, Brekken RA, Sage EH, Ambati BK, Ambati J
    J. Clin. Invest., 2006-02-01;116(2):422-9.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  25. Functional analysis of the matricellular protein SPARC with novel monoclonal antibodies.
    Authors: Sweetwyne MT, Brekken RA, Workman G, Bradshaw AD, Carbon J, Siadak AW, Murri C, Sage EH
    J. Histochem. Cytochem., 2004-06-01;52(6):723-33.
    Species: Human
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-P, Western Blot

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Reviews for Mouse SPARC Antibody

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Mouse SPARC Antibody
By Emma Jones on 02/02/2018
Application: WB Sample Tested: Mouse hippocampal tissue Species: Mouse

Used at 1:3000 in 5% BSA and secondary goat-HRP antibody (R&D) at 1:5000

Western Blot Mouse SPARC Antibody AF942
Mouse SPARC Antibody
By Anonymous on 01/12/2018
Application: WB Sample Tested: trabecular meshwork Species: Mouse

mouse SPARC was overexpressed by adeno-SPARC in mouse trabecular meshork (TM) cells.

Western Blot Mouse SPARC Antibody AF942
Mouse SPARC Antibody
By Anonymous on 05/18/2017
Application: IHC Sample Tested: Colon cancer tissue,Colon tissue Species: Mouse

Worked well in methacarn fixed tissues

Immunohistochemistry Mouse SPARC Antibody AF942