Mouse Syndecan-3 Biotinylated Antibody

Catalog #: BAF2734 Datasheet / COA / SDS

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BAF2734

All Syndecan-3 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Syndecan-3 Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Syndecan-3 in Western blots. In Western blots, less than 2% cross-reactivity with recombinant mouse (rm) Syndecan-1, recombinant human (rh) Syndecan-2, rhSyndecan-3, and rmSyndecan-4 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Syndecan-3
Ala45-Glu384
Accession # Q64519

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Syndecan‑3

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Syndecan-3

Mouse Syndecan-3 (also known as N-Syndecan) is a variably glycosylated type I transmembrane (TM) protein that belongs to the Syndecan family. It is synthesized as a 442 amino acid (aa) precursor with a 45 aa signal sequence, a 339 aa extracellular domain (ECD), a 25 aa TM segment and a 33 aa cytoplasmic region. It presumably exists as a dimer. Heparan sulfate modification of the ECD yields a native molecular weight that exceeds 200 kDa. Soluble forms generated by proteolytic processing are suggested to exist. Syndecan-3 is a musculoskeletal and neuronal molecule which mediates neurite outgrowth and myoblast differentiation. Mouse Syndecan-3 ECD shares 95% and 81% aa sequence identity with rat and human Syndecan-3 ECD, respectively.

Entrez Gene IDs

9672 (Human); 20970 (Mouse)

Alternate Names

KIAA0468; N-Syndecan; SDC3; SDCN; SYND3syndecan proteoglycan 3; syndecan 3 (N-syndecan); syndecan 3; syndecan neural type; Syndecan3; Syndecan-3