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Mouse TER-119 Biotinylated Antibody

Catalog #: BAM1125
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
BAM1125
TER‑119 in Mouse Embryo.
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Citations (1)
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Mouse TER-119 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse TER‑119 in Western blots. This antibody has been shown to react with cells of the erythroid lineage in embryonic yolk sac, fetal liver, adult bone marrow, adult peripheral blood, and adult lymphoid organs (Ikuta, K. et al. (1990) Cell 62:863). TER-119 Antigen is a molecule associated with cell-surface glycophorin A (Kina, T. et al. (2000) Br. J. Haematol. 109:280). The antibody has been shown to react with erythroid cells at differentiation stages from early proerythroblast to mature erythrocyte (Kina, T. et al. (2000) Br. J. Haematol. 109:280).
Source
Monoclonal Rat IgG2B Clone # TER-119
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
C57BL/6 mouse day-14 fetal liver cells
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
Kina, T. et al. (2000) Br. J. Haematol. 109:280
 
Flow Cytometry
0.25 µg/106 cells
Mouse bone marrow cells
Immunohistochemistry
8-25 µg/mL
See below
Immunoprecipitation
Kina, T. et al. (2000) Br. J. Haematol. 109:280
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry TER-119 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

TER‑119 in Mouse Embryo. TER-119 was detected in immersion fixed frozen sections of mouse embryo (E15) using Rat Anti-Mouse TER-119 Biotinylated Monoclonal Antibody (Catalog # BAM1125) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counter-stained with hematoxylin (blue). Specific staining was localized to red blood cell plasma membranes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TER-119

The TER-119 Antigen has been used as a marker for erythroid cells from the early proerythroblast to mature erythrocyte stages of development. The 52-kDa TER-119 Antigen is associated with Glycophorin A on erythrocytes.

Entrez Gene IDs
104231 (Mouse)
Alternate Names
Ly76; Ly-76; TER119; TER-119

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Citation for Mouse TER-119 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Hepcidin regulates ferroportin expression and intracellular iron homeostasis of erythroblasts.
    Authors: Zhang DL, Senecal T, Ghosh MC
    Blood, 2011-06-23;118(10):2868-77.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Immunoprecipitation

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