Mouse Thrombopoietin R/Tpo R Antibody

Catalog #: AF1317 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF1317
AF1317-SP

Product Details

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Thrombopoietin R/Tpo R Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Thrombopoietin R/Tpo R in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human Tpo R is observed and less than 2% cross-reactivity with recombinant mouse Epo R is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Thrombopoietin R/Tpo R
Ser18-Trp483
Accession # CAA52031

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Thrombopoietin R/Tpo R Fc Chimera (Catalog # 1317-TR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Thrombopoietin R/Tpo R

Thrombopoietin receptor (Tpo R), also known as myeloproliferative leukemia protein (c-mpl), is a type I membrane protein that is a member of the hematopoietin/cytokine receptor superfamily. Thrombopoietin (Tpo) is a key regulator of megakaryocytopoiesis and thrombopoiesis in vitro and in vivo. Receptor dimerization occurs upon ligand binding. Downstream signaling cascades utilize the Ras/MAP and JAK/STAT pathways (1). In humans, variations at the 3’ end on the molecule result in the formation of 3 distinct mRNA species, a P-form, a K-form, and a potentially secreted form. The predominant form in humans is the P-form, which encodes a full length receptor. In mouse, mRNA for a full length form, a potentially secreted form, and a form lacking 8 amino acids in the extra-cytoplasmic domain has been detected (2). The human and mouse receptors share approximately 81% amino acid identity. Tpo R is expressed at low levels in various cell types including hematopoietic progenitor cells, megakaryocytes and platelets.

References

Kuter, D.J. and J. Li (2001) in Cytokine Reference, p. 1953. Oppenhiem, J.J. and M. Feldmann eds. Academic Press.
Kaushansky, K. and J.G. Drachman (2002) Oncogene 21:3359.

Long Name

Thrombopoietin Receptor

Entrez Gene IDs

4352 (Human); 17480 (Mouse)

Alternate Names

CD110 antigen; CD110; c-Mpl; MPL; MPLV; Myeloproliferative leukemia protein; myeloproliferative leukemia virus oncogene; Proto-oncogene c-Mpl; Thrombopoietin R; thrombopoietin receptor; TpoR; TPO-R; TPORMPLV