Mouse TIGIT Antibody Summary
Met1-Thr143
Accession # P86176
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
View Larger
Detection of TIGIT on CD4+ Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were surface stained with Rat anti-Mouse CD4 FITC-conjugated Monoclonal Antibody (Catalog # FAB554F) and either (A) Rabbit Anti-Mouse TIGIT Monoclonal Antibody (Catalog # MAB72671) or (B) Rabbit IgG control antibody (Catalog # MAB1050) followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). Then stained with Rabbit anti-Human/Mouse FoxP3 PE-conjugated Monoclonal Antibody (Catalog # IC8214P).
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TIGIT
TIGIT (T cell Immunoreceptor with Ig and ITIM domains; also Vstm3 and Vsig9) is a 30-34 kDa (in human) member of the CD28 family, Ig superfamily of molecules. It is expressed by NK cells and multiple subsets of mature T cells, and binds to PVR/CD155 and PVR2/CD112 that appear on dendritic cells (DC) and endothelium. Along with CD226, the TIGIT:CD226/DNAM1 and PVR:PVR2 pairings appear to form a network that parallels the well-characterized B7-1:B7-2 and CD28:CTLA4 system. Binding of TIGIT by DC induces DC IL-10 release and inhibits IL-12 production. Ligation of TIGIT on T cells dampens TCR-mediated activation, while NK cell TIGIT ligation blocks NK cell cytotoxicity. Mature mouse TIGIT is a type I transmembrane protein 215 amino acids (aa) in length. It contains a 116 aa extracellular region (aa 26-141) with a V-type Ig-like domain (aa 27-125), and a 79 aa cytoplasmic domain with one ITIM motif. There is one isoform variant that is quite unusual and shows an addition of nine amino acids spread over three insertion sites (SwissProt #:P86176). Mouse and human TIGIT are highly divergent, and over aa 26-143, mouse TIGIT shares only 68% aa sequence identity with human TIGIT.
Product Datasheets
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Mouse TIGIT Antibody
Average Rating: 5 (Based on 2 Reviews)
Have you used Mouse TIGIT Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
Antibodies primary and secondary incubated for 60 min at 4 C. The antibody was tested along rabbit isotype control. The analysis was done in FL1 channel using flow cytometry
