Mouse TLR4 PE-conjugated Antibody

Catalog #: FAB2759P
1 Citation Datasheet / COA / SDS

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FAB2759P

Detection of TLR4 in RAW 264.7 Mouse Cell Line by Flow Cytometry.

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All TLR4 Antibodies

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Mouse TLR4 PE-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse TLR4 in direct ELISAs and Western blots. In direct ELISAs, does not cross-react with recombinant mouse TLR1, 2, 3, 6, or recombinant human TLR4.

Source

Monoclonal Rat IgG_2A Clone # 267518

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse TLR4 Asn26-Lys629 Accession # Q9QUK6

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of TLR4 in RAW 264.7 Mouse Cell Line by Flow Cytometry. LPS-activated RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse TLR4 PE-conjugated Monoclonal Antibody (Catalog # FAB2759P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 degreesC as supplied.

Background: TLR4

TLR4 belongs to the IL-1 receptor/Toll-like receptor superfamily. It is expressed in placenta and peripheral blood leukocytes. TLR4 is the pattern recognition receptor for LPS.

Long Name

Toll-like Receptor 4

Entrez Gene IDs

7099 (Human); 21898 (Mouse); 29260 (Rat)

Alternate Names

ARMD10; CD_antigen: CD284; CD284 antigen; CD284; EC 3.2.2.6; EC:3.2.2.6; homolog of Drosophila toll; hToll; TLR4; TLR-4; toll like receptor 4 protein; TOLL; toll-like receptor 4

Product Datasheets

Product Datasheet

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Related Research Areas

Citation for Mouse TLR4 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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