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Mouse TREM2 Biotinylated Antibody

Catalog #: BAF1729
15 Citations Datasheet / COA / SDS
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BAF1729
TREM‑2 in Mouse Splenocytes.
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Product Details
Citations (15)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse TREM2 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse TREM2 in Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human (rh) TREM2 is observed and less than 1% cross-reactivity with recombinant mouse (rm) TREM1 and rmTREM3 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse TREM2b
Leu19-Pro168
Accession # Q99NH8
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse TREM2b Fc Chimera (Catalog # 1729-T2)
ELISA

This antibody functions as an ELISA detection antibody when paired with Rat Anti-Human/Mouse TREM2 Monoclonal Antibody (Catalog # MAB17291) or Sheep Anti-Mouse TREM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1729)

This product is intended for assay development on various assay platforms requiring antibody pairs.

 
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry TREM-2 antibody in Mouse Splenocytes by Immunocytochemistry (ICC). View Larger

TREM‑2 in Mouse Splenocytes. TREM-2 was detected in immersion fixed mouse spleno-cytes using Mouse Anti-TREM-2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1729) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (yellow; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

ELISA Mouse TREM2 Antibody in ELISA Standard Curve. View Larger

Mouse TREM2 ELISA Standard Curve. Recombinant Human/Mouse TREM2 protein was serially diluted 2-fold and captured by Rat Anti-Human/Mouse TREM2 Monoclonal Antibody (Catalog # MAB17291) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Mouse TREM2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1729) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

ELISA Mouse TREM2 Antibody in ELISA Standard Curve. View Larger

Mouse TREM2 ELISA Standard Curve. Recombinant Mouse TREM2 protein was serially diluted 2-fold and captured by Sheep Anti-Mouse TREM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1729) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Mouse TREM2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1729) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TREM2

TREM2 (Triggering Receptor Expressed by Myeloid cells) is an Ig superfamily cell surface receptor that activates a number of myeloid cell types (1). It is a member of a small gene family located on human chromosome 6p21 and mouse chromosome 17 in a region linked to the MHC (2). A single human TREM2 gene has been described, however, two closely related orthologs were reported in mouse (3). The proteins differ by only three amino acids and were designated TREM2a and TREM2b. TREM2 is type I transmembrane protein consisting of a single extracellular immunoglobulin (V-like) domain, a transmembrane domain with a positively charged lysine residue, and a short cytoplasmic tail (1). It associates with the signal adapter protein, DAP12, for signaling and function. DAP12 has a cytoplasmic ITAM that is phosphorylated upon ligand binding leading to the subsequent activation of cytoplasmic tyrosine kinases. TREM2 is expressed by immature monocyte-derived dendritic cells (DC), and expression is down-regulated upon activation of DC by microbial products and costimulatory signals (4). Ligation of TREM2 on immature DC with anti-TREM2 antibodies results in partial DC activation and the up-regulation of CCR7 and some co-stimulatory molecules. A role for TREM2 in the functioning of osteoclasts and microglia is suggested by the discovery that homozygous loss-of-function mutations in either TREM2 or DAP12 result in Nasu-Hakola disease characterized by a combination of presenile demetia and bone cysts (5). In vitro studies indicate that the differentiation of myeloid precursors into osteoclasts is dramatically impaired in TREM2 deficient individuals (6).

References
  1. Colonna, M. (2003) Nature Rev. Immunol. 3:445.
  2. Allcock, R. et al. (2003) Eur. J. Immunol. 33:567.
  3. Daws, M. et al. (2001) Eur. J. Immunol. 31:783.
  4. Bouchon, A. et al. (2001) J. Exp. Med. 194:1111.
  5. Paloneva, J. et al. (2002) Am. J. Hum. Genet. 71:656.
  6. Cella, M. et al. (2003) J. Exp. Med. 198:645.
Long Name
Triggering Receptor Expressed on Myeloid Cells 2
Entrez Gene IDs
54209 (Human); 102133279 (Cynomolgus Monkey)
Alternate Names
PLOSL2; TREM2; TREM-2; Trem2a; Trem2b; Trem2c; TREM-2triggering receptor expressed on myeloid cells 2a; Triggering receptor expressed on monocytes 2; triggering receptor expressed on myeloid cells 2

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Citations for Mouse TREM2 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. Negative regulation of TREM2-mediated C9orf72 poly-GA clearance by the NLRP3 inflammasome
    Authors: X Shu, C Wei, WY Tu, K Zhong, S Qi, A Wang, L Bai, SX Zhang, B Luo, ZZ Xu, K Zhang, C Shen
    Cell Reports, 2023-02-16;42(2):112133.
    Species: Mouse
    Sample Types: Cerebrospinal Fluid
    Applications: ELISA Detection
  2. MS4A4A modifies the risk of Alzheimer disease by regulating lipid metabolism and immune response in a unique microglia state
    Authors: SF You, L Brase, F Filipello, AK Iyer, J Del-Aguila, J He, R D'Oliveira, J Budde, J Norton, J Gentsch, NM Dräger, SM Sattler, M Kampmann, L Piccio, JC Morris, RJ Perrin, E McDade, Dominantly, SM Paul, AG Cashikar, BA Benitez, O Harari, CM Karch
    medRxiv : the preprint server for health sciences, 2023-02-08;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Capture
  3. Experimental evidence for temporal uncoupling of brain Abeta deposition and neurodegenerative sequelae
    Authors: C Rother, RE Uhlmann, SA Müller, J Schelle, A Skodras, U Obermüller, LM Häsler, M Lambert, F Baumann, Y Xu, C Bergmann, G Salvadori, M Loos, I Brzak, D Shimshek, U Neumann, Dominantly, LC Walker, SA Schultz, JP Chhatwal, SA Kaeser, SF Lichtentha, M Staufenbie, M Jucker
    Nature Communications, 2022-11-28;13(1):7333.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Capture
  4. Reduction of alphaSYN Pathology in a Mouse Model of PD Using a Brain-Penetrating Bispecific Antibody
    Authors: S Roshanbin, U Julku, M Xiong, J Eriksson, E Masliah, G Hultqvist, J Bergström, M Ingelsson, S Syvänen, D Sehlin
    Pharmaceutics, 2022-07-05;14(7):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Detection
  5. Novel App knock-in mouse model shows key features of amyloid pathology and reveals profound metabolic dysregulation of microglia
    Authors: D Xia, S Lianoglou, T Sandmann, M Calvert, JH Suh, E Thomsen, J Dugas, ME Pizzo, SL DeVos, TK Earr, CC Lin, S Davis, C Ha, AW Leung, H Nguyen, R Chau, E Yulyanings, I Lopez, H Solanoy, ST Masoud, CC Liang, K Lin, G Astarita, N Khoury, JY Zuchero, RG Thorne, K Shen, S Miller, JJ Palop, D Garceau, M Sasner, JD Whitesell, JA Harris, S Hummel, J Gnörich, K Wind, L Kunze, A Zatcepin, M Brendel, M Willem, C Haass, D Barnett, TS Zimmer, AG Orr, K Scearce-Le, JW Lewcock, G Di Paolo, PE Sanchez
    Molecular Neurodegeneration, 2022-06-11;17(1):41.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Capture
  6. Microglia use TAM receptors to detect and engulf amyloid &beta plaques
    Authors: Y Huang, KE Happonen, PG Burrola, C O'Connor, N Hah, L Huang, A Nimmerjahn, G Lemke
    Nature Immunology, 2021-04-15;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Microglial Activation in the Right Amygdala-Entorhinal-Hippocampal Complex is Associated with Preserved Spatial Learning in AppNL-G-F mice
    Authors: G Biechele, K Wind, T Blume, C Sacher, L Beyer, F Eckenweber, N Franzmeier, M Ewers, B Zott, S Lindner, FJ Gildehaus, B von Ungern, S Tahirovic, M Willem, P Bartenstei, P Cumming, A Rominger, J Herms, M Brendel
    Neuroimage, 2020-12-29;0(0):117707.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ELISA Development
  8. Trem2 Y38C mutation and loss of Trem2 impairs neuronal synapses in adult mice
    Authors: VS Jadhav, PBC Lin, T Pennington, GV Di Prisco, AJ Jannu, G Xu, M Moutinho, J Zhang, BK Atwood, SS Puntambeka, SJ Bissel, AL Oblak, GE Landreth, BT Lamb
    Mol Neurodegener, 2020-10-28;15(1):62.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ELISA Detection
  9. Enhancing protective microglial activities with a dual function TREM2 antibody to the stalk region
    Authors: K Schlepckow, KM Monroe, G Kleinberge, L Cantuti-Ca, S Parhizkar, D Xia, M Willem, G Werner, N Pettkus, B Brunner, A Sülzen, B Nuscher, H Hampel, X Xiang, R Feederle, S Tahirovic, JI Park, R Prorok, C Mahon, CC Liang, J Shi, DJ Kim, H Sabelström, F Huang, G Di Paolo, M Simons, JW Lewcock, C Haass
    EMBO Mol Med, 2020-03-10;0(0):e11227.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Capture
  10. Opposite microglial activation stages upon loss of PGRN or TREM2 result in reduced cerebral glucose metabolism
    Authors: JK Götzl, M Brendel, G Werner, S Parhizkar, L Sebastian, G Kleinberge, AV Colombo, M Deussing, M Wagner, J Winkelmann, J Diehl-Schm, J Levin, K Fellerer, A Reifschnei, S Bultmann, P Bartenstei, A Rominger, S Tahirovic, ST Smith, C Madore, O Butovsky, A Capell, C Haass
    EMBO Mol Med, 2019-06-01;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Capture
  11. Soluble TREM2 ameliorates pathological phenotypes by modulating microglial functions in an Alzheimer's disease model
    Authors: L Zhong, Y Xu, R Zhuo, T Wang, K Wang, R Huang, D Wang, Y Gao, Y Zhu, X Sheng, K Chen, N Wang, L Zhu, D Can, Y Marten, M Shinohara, CC Liu, D Du, H Sun, L Wen, H Xu, G Bu, XF Chen
    Nat Commun, 2019-03-25;10(1):1365.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Detection)
  12. The FTD-like syndrome causing TREM2 T66M mutation impairs microglia function, brain perfusion, and glucose metabolism
    Authors: G Kleinberge, M Brendel, E Mracsko, B Wefers, L Groeneweg, X Xiang, C Focke, M Deu beta ing, M Suárez-Cal, F Mazaheri, S Parhizkar, N Pettkus, W Wurst, R Feederle, P Bartenstei, T Mueggler, T Arzberger, I Knuesel, A Rominger, C Haass
    EMBO J., 2017-05-30;0(0):.
    Species: Mouse
    Sample Types: CSF, Tissue Homogenates
    Applications: ELISA Development, Immunoprecipitation
  13. Increase of TREM2 during Aging of an Alzheimer's Disease Mouse Model Is Paralleled by Microglial Activation and Amyloidosis
    Authors: M Brendel, G Kleinberge, F Probst, A Jaworska, F Overhoff, T Blume, NL Albert, J Carlsen, S Lindner, FJ Gildehaus, L Ozmen, M Suárez-Cal, P Bartenstei, K Baumann, M Ewers, J Herms, C Haass, A Rominger
    Front Aging Neurosci, 2017-01-31;9(0):8.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Development (Capture), Western Blot
  14. The triggering receptor expressed on myeloid cells 2 inhibits complement component 1q effector mechanisms and exerts detrimental effects during pneumococcal pneumonia.
    Authors: Sharif O, Gawish R, Warszawska J, Martins R, Lakovits K, Hladik A, Doninger B, Brunner J, Korosec A, Schwarzenbacher R, Berg T, Kralovics R, Colinge J, Mesteri I, Gilfillan S, Salmaggi A, Verschoor A, Colonna M, Knapp S
    PLoS Pathog, 2014-06-12;10(6):e1004167.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  15. DAP12 is required for macrophage recruitment to the lung in response to cigarette smoke and chemotaxis toward CCL2.
    Authors: Koth LL, Cambier CJ, Ellwanger A
    J. Immunol., 2010-04-26;184(11):6522-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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