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Mouse Tryptase beta-1/MCP-7/Mcpt7 Antibody

Catalog #: AF1937
5 Citations Datasheet / COA / SDS
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AF1937
AF1937-SP
Product Details
Citations (5)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Tryptase beta-1/MCP-7/Mcpt7 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Tryptase beta ‑1/MCP‑7/Mcpt7 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross‑reactivity with recombinant human (rh) TPSB2 is observed, 5% cross-reactivity with rhTPS1 is observed, and less than 1% cross-reactivity with rhTPSG1 and recombinant mouse MCPT1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Tryptase beta ‑1/MCP‑7/Mcpt7
Ile29-Phe273
Accession # Q02844
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Purity
Immunogen affinity purified

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Tryptase  beta ‑1/MCP‑7/Mcpt7 (Catalog # 1937-SE)
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Mouse Tryptase  beta ‑1/MCP‑7/Mcpt7 (Catalog # 1937-SE), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Tryptase beta-1/MCP-7

Tryptase beta -1 is a serine protease with trypsin-like activity, which is sometimes also referred to as Mast Cell Protease 7 (1). It is stored in the secretory granules of mouse mast cells (2). It exhibits anticoagulant activity due to its ability to degrade fibrinogen in the presence of the diverse array of protease inhibitors in plasma (3). The deduced amino acid sequence for mouse Tryptase beta -1 consists of a signal peptide (residues 1‑18), a pro region (residues 19‑28) and the mature chain (residue 29‑273). The mature chain was expressed and purified.

References
  1. McNeil, H.P. et al. (1992) Proc. Natl. Acad. Sci. USA 89:11174.
  2. Matsumoto, R et al. (1995) J. Biol. Chem. 270:19524.
  3. Huang, C. et al. (1997) J. Biol. Chem. 272:31885.
Long Name
Mast Cell Protease 7
Entrez Gene IDs
100503895 (Mouse)
Alternate Names
Mast Cell Tryptase; Mcp7; MCP-7; Mcpt7; TPSB1; TRYB1; Tryptase beta1; Tryptase beta-1

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Citations for Mouse Tryptase beta-1/MCP-7/Mcpt7 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Dendritic cell immunoreceptor drives atopic dermatitis by modulating oxidized CaMKII-involved mast cell activation
    Authors: X Luo, J Chen, H Yang, X Hu, MP Alphonse, Y Shen, Y Kawakami, X Zhou, W Tu, T Kawakami, M Wan, NK Archer, H Wang, P Gao
    JCI Insight, 2022-03-08;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Epidermal RAF prevents allergic skin disease
    Elife, 2016-07-19;5(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  3. Immunotherapy using algal-produced Ara h 1 core domain suppresses peanut allergy in mice
    Authors: M Cecilia Berin
    Plant Biotechnol. J., 2016-01-23;14(7):1541-50.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Development (Capture)
  4. Blockade of mast cell activation reduces cutaneous scar formation.
    Authors: Chen L, Schrementi M, Ranzer M, Wilgus T, DiPietro L
    PLoS ONE, 2014-01-22;9(1):e85226.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  5. Phagocytosis-independent antimicrobial activity of mast cells by means of extracellular trap formation.
    Authors: von Kockritz-Blickwede M, Goldmann O, Thulin P, Heinemann K, Norrby-Teglund A, Rohde M, Medina E
    Blood, 2008-01-08;111(6):3070-80.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC

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