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Mouse TSLPR Antibody

Catalog #: AF546
6 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF546
AF546-SP
Detection of TSLP R in Mouse Splenocytes by Flow Cytometry.
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Product Details
Citations (6)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse TSLPR Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse TSLP R in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse TSLP R
Ala20-Leu233
Accession # BAA92159
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Purity
Immunogen affinity purified

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse TSLP R Fc Chimera (Catalog # 546-TR)
Flow Cytometry
2.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Neutralization
Measured by its ability to neutralize TSLP-induced proliferation in the BaF3 mouse pro‑B cell line transfected with mouse IL‑7 R alpha /CD127. Park, L.S. et al. (2000) J. Exp. Med. 192:659. The Neutralization Dose (ND50) is typically 0.2-0.6 µg/mL in the presence of 7.5 ng/mL Recombinant Mouse TSLP.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of TSLP R antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of TSLP R in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Goat Anti-Mouse TSLP R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF546, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).

Neutralization Cell Proliferation Induced by TSLP and Neutralization by Mouse TSLP R Antibody. View Larger

Cell Proliferation Induced by TSLP and Neutralization by Mouse TSLP R Antibody. Recombinant Mouse TSLP (Catalog # 555-TS) stimulates proliferation in the BaF3 mouse pro-B cell line transfected with mouse IL-7 Ra/CD127 in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse TSLP (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse TSLP R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF546). The ND50 is typically 0.2-0.6 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TSLPR

TSLP R, also named Delta (1) and CRLM-2 (2) (cytokine receptor-like module-2), was originally cloned as a novel type 1 cytokine receptor with similarity to the common gamma chain. It was subsequently identified to be a subunit of the cellular receptor for the IL-7-like cytokine TSLP and termed TSLP R (3). The TSLP R cDNA encodes a 359 amino acid (aa) residue type 1 membrane protein with a 24 aa residue signal peptide, a 206 aa residue extracellular domain that showed 24% sequence identity with the mouse common gamma receptor, a 23 aa residue transmembrane domain, and a 106 aa residue cytoplasmic domain. The cytoplasmic domain of TSLP R contains a membrane-proximal box1 motif which is known to be important for association with JAKs. An alternatively spliced mRNA variant encoding a soluble TSLP R has also been reported (2). TSLP R expression is ubiquitous in the immune and hematopoietic cells, but is up-regulated in Th2-skewed cells. Cells expressing TSLP R alone bind TSLP with low affinity. Co-expression of TSLP R and IL-7 R alpha is required for high-affinity TSLP binding and signal transduction (3, 4).

References
  1. Fujio, K. et al. (2000) Blood 95:2204.
  2. Hiroyama, T. et al. (2000) Biochem. Biophys. Res. Commun. 272:224.
  3. Park, L.S. et al. (2000) J. Exp. Med. 192:659.
  4. Pandey, A. et al. (2000) Nat. Immunol. 1:59.
Long Name
Thymic Stromal Lymphopoietin Receptor
Entrez Gene IDs
64109 (Human); 57914 (Mouse); 171499 (Rat)
Alternate Names
CRL2; CRL2cytokine receptor CRL2 precusor; CRLF2; CRLF2Y; CRLM-2; Cytokine receptor-like 2; cytokine receptor-like factor 2; ILXR; IL-XR; P2RY8/CRLF2 fusion; PCOR1; Thymic stromal lymphopoietin protein receptor; thymic stromal lymphopoietin receptor; thymic stromal-derived lymphopoietin receptor; TSLP R; TSLP receptor; TSLPR

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Citations for Mouse TSLPR Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Type 2 immunity plays an essential role for murine model of allergic contact dermatitis with mixed type 1/type 2 immune response
    Authors: J Park, JW Lee, SH Kim, J Oh, WS Roh, SM Kim, CO Park, MG Lee, TG Kim
    Journal of dermatological science, 2021-10-06;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. TSLP Protects Corneas From Pseudomonas aeruginosa Infection by Regulating Dendritic Cells and IL-23-IL-17 Pathway
    Authors: X Cui, N Gao, R Me, J Xu, FX Yu
    Invest. Ophthalmol. Vis. Sci., 2018-08-01;59(10):4228-4237.
    Species: Mouse
    Sample Types: In Vivo, Tissue Homogenates, Whole Tissue
    Applications: IHC, Neutralization, Western Blot
  3. Immune Characterization of Bone Marrow-Derived Models of Mucosal and Connective Tissue Mast Cells
    Authors: S Benedé, E Cody, C Agashe, MC Berin
    Allergy Asthma Immunol Res, 2018-05-01;10(3):268-277.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. IL-7R? Expression Regulates Murine Dendritic Cell Sensitivity to Thymic Stromal Lymphopoietin
    Authors: L Kummola, Z Ortutay, X Chen, S Caucheteux, S Hämäläinen, S Aittomäki, R Yagi, J Zhu, M Pesu, WE Paul, IS Junttila
    J. Immunol., 2017-04-12;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Rearrangement of CRLF2 in B-progenitor- and Down syndrome-associated acute lymphoblastic leukemia.
    Authors: Mullighan CG, Collins-Underwood JR, Phillips LA, Loudin MG, Liu W, Zhang J, Ma J, Coustan-Smith E, Harvey RC, Willman CL, Mikhail FM, Meyer J, Carroll AJ, Williams RT, Cheng J, Heerema NA, Basso G, Pession A, Pui CH, Raimondi SC, Hunger SP, Downing JR, Carroll WL, Rabin KR
    Nat. Genet., 2009-10-18;41(11):1243-6.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Overexpression of murine TSLP impairs lymphopoiesis and myelopoiesis.
    Authors: Osborn MJ, Ryan PL, Kirchhof N, Panoskaltsis-Mortari A, Mortari F, Tudor KS
    Blood, 2003-09-25;103(3):843-51.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization

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