Mouse TWEAK R/TNFRSF12 Biotinylated Antibody

Catalog #: BAF1610 Datasheet / COA / SDS

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BAF1610

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Mouse TWEAK R/TNFRSF12 Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse TWEAK R/TNFRSF12 in ELISAs and Western blots. In sandwich immunoassays, less than 2% cross‑reactivity with recombinant human TWEAK R is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse TWEAK R/TNFRSF12
Glu28-Trp79
Accession # Q9CR75

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Purity

Antigen Affinity-purified

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse TWEAK R/TNFRSF12 Fc Chimera (Catalog # 1610-TW)

Mouse TWEAK R/TNFRSF12 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: Mouse TWEAK R/TNFRSF12 Antibody (Catalog # AF1610)

Standard: Recombinant Mouse TWEAK R/TNFRSF12 Fc Chimera Protein, CF (Catalog # 1610-TW)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TWEAK R/TNFRSF12

TNF-related weak inducer of apoptosis receptor (TWEAK R) belongs to the TNF receptor superfamily and is designated TNFRSF12. The gene for TWEAK R was originally identified as a fibroblast growth factor-inducible immediate-early response gene Fn14 in mouse NIH 3T3 fibroblasts (1, 2). Mouse TWEAK R cDNA encodes a 129 amino acid (aa) residues type I transmembrane protein with a 27 aa signal peptide, a 53 aa extracellular domain, a 21 aa transmembrane domain, and a 28 aa cytoplasmic domain (1-3). Human and mouse TWEAK R share 82% aa sequence identity. TWEAK R is the smallest member of the TNF receptor superfamily and contains only one cysteine-rich region in its extracellular domain. The TWEAK R cytoplasmic domain contains one TRAF binding motif which binds TRAFs 1, 2, and 3. TWEAK R binds its ligand TWEAK/TNFSF12 with high affinity to initiate a signal transduction cascade that depending upon the cell type, may lead to a variety of cellular responses including cell death by both caspase-dependent apoptosis and cathepsin B-dependent necrosis, cell proliferation, and angiogenesis (2-6). In newborn mice, TWEAK R is highly expressed in all tissues examined (heart, intestine, kidney, liver, lung, and skin) (1). In adult mice, high TWEAK R expression levels are found in the heart and ovary, while lower expression levels are detected in the lung, kidney, skin. Elevated levels of TWEAK R mRNA were found in human or mouse hepatocellular carcinoma specimens, in regenerating mouse liver and in injured rat arteries (2, 3).

References

Meighan-Mantha, R. et al. (1999) J. Biol. Chem. 274:33166.
Feng, S. et al. (2000) Am. J. Pathol. 156:1253.
Wiley, S. et al. (2001) Immunity 15:837.
Schneider, P. et al. (1999) Eur. J. Immunol. 29:1785.
Nakayama, M. et al. (2002) J. Immunol. 168:734.
Lynch, C.N. et al. (1999) J. Biol. Chem. 274:8455.

Long Name

TNF-like and Weak Inducer of Apoptosis Receptor

Entrez Gene IDs

51330 (Human); 27279 (Mouse)

Alternate Names

CD266 antigen; CD266; FGF-inducible 14; Fibroblast growth factor-inducible immediate-early response protein 14; Fn14; FN14tumor necrosis factor receptor superfamily member 12A; TNFRSF12; TNFRSF12A; tumor necrosis factor receptor superfamily, member 12A; TWEAK R; TWEAKR; tweak-receptor; type I transmembrane protein Fn14