Mouse VEGF_120/164 Antibody

Catalog #: MAB493
10 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB493
MAB493-SP

All VEGF Antibodies

Product Details

Citations (10)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse VEGF_120/164 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse VEGF_120/164 in direct ELISAs and Western blots. This antibody does not recognize recombinant mouse VEGF₁₁₅or any isoform of human VEGF.

Source

Monoclonal Rat IgG_2B Clone # 39917

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse VEGF₁₆₄
Ala27-Arg190
Accession # AAA40547

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse VEGF₁₂₀ (Catalog # 494-VE)
Recombinant Mouse VEGF₁₆₄ (Catalog # 493-MV)
under non-reducing conditions only

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VEGF

Vascular Endothelial Growth Factor (VEGF or VEGF-A), also known as Vascular Permeability Factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues and a cystine knot structure. VEGF164 appears to be the most abundant and potent isoform, followed by VEGF120 and VEGF188. Mouse VEGF164 is an approximately 50 kDa molecular weight homodimer sharing 97% aa sequence identity with corresponding regions of rat, 89% with human and porcine, 90% with feline, equine and canine, and 88% with bovine VEGF, respectively. VEGF binds the type I transmembrane receptor tyrosine kinases VEGF R1 (also called Flt-1) and VEGF R2 (Flk-1/KDR) on endothelial cells. Although VEGF affinity is highest for binding to VEGF R1, VEGF R2 appears to be the primary mediator of VEGF angiogenic activity. Human VEGF165 binds the Semaphorin receptor, Neuropilin-1 and promotes complex formation with VEGF R2. VEGF is required during embryogenesis and functions to regulate the proliferation, migration, and survival of endothelial cells. In adults, VEGF functions mainly in wound healing and the female reproductive cycle. Pathologically, it is involved in tumor angiogenesis and vascular leakage. Circulating VEGF levels correlate with disease activity in autoimmune diseases such as rheumatoid arthritis, multiple sclerosis and systemic lupus erythematosus. VEGF is induced by hypoxia and cytokines such as IL-1, IL-6, IL-8, Oncostatin M (OSM) and TNF-alpha.

Long Name

Vascular Endothelial Growth Factor

Entrez Gene IDs

7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish)

Alternate Names

MVCD1; VAS; vascular endothelial growth factor A; Vascular permeability factor; Vasculotropin; VEGF; VEGFA; VEGF-A; VEGFMGC70609; VPF; VPFvascular endothelial growth factor

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse VEGF_120/164 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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Monocyte-derived IL-6 programs microglia to rebuild damaged brain vasculature
Authors: Choi, BR;Johnson, KR;Maric, D;McGavern, DB;
Nature immunology
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
Direct thrombin inhibitors as alternatives to heparin to preserve lung growth and function in a murine model of compensatory lung growth
Authors: ST Tsikis, TI Hirsch, SC Fligor, A Pan, MM Joiner, A Devietro, PD Mitchell, H Kishikawa, KM Gura, M Puder
Scientific Reports, 2022-12-07;12(1):21117.
Species: Mouse
Sample Types: Tissue Lysates
Applications: Western Blot
Lipopolysaccharide-induced murine lung injury results in long-term pulmonary changes and downregulation of angiogenic pathways
Authors: ST Tsikis, SC Fligor, TI Hirsch, A Pan, LJ Yu, H Kishikawa, MM Joiner, PD Mitchell, M Puder
Oncogene, 2022-06-17;12(1):10245.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
Investigation of the mechanisms of VEGF-mediated compensatory lung growth: the role of the VEGF heparin-binding domain
Authors: LJ Yu, VH Ko, DT Dao, JD Secor, A Pan, BS Cho, PD Mitchell, H Kishikawa, DR Bielenberg, M Puder
Scientific Reports, 2021-06-04;11(1):11827.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot
Temporally distinct myeloid cell responses mediate damage and repair after cerebrovascular injury
Authors: P Mastorakos, N Mihelson, M Luby, SR Burks, K Johnson, AW Hsia, J Witko, JA Frank, L Latour, DB McGavern
Nature Neuroscience, 2021-01-18;24(2):245-258.
Species: Mouse
Sample Types: Whole Cells, Whole Tissue
Applications: Flow Cytometry, IHC
A paradoxical method to enhance compensatory lung growth: Utilizing a VEGF inhibitor
Authors: DT Dao, L Anez-Busti, SS Jabbouri, A Pan, H Kishikawa, PD Mitchell, GL Fell, MA Baker, RS Watnick, H Chen, MS Rogers, DR Bielenberg, M Puder
PLoS ONE, 2018-12-19;13(12):e0208579.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC-P, Western Blot
The Novel Mechanisms Concerning the Inhibitions of Palmitate-Induced Proinflammatory Factor Releases and Endogenous Cellular Stress with Astaxanthin on MIN6 ?-Cells
Authors: A Kitahara, K Takahashi, N Morita, T Murashima, H Onuma, Y Sumitani, T Tanaka, T Kondo, T Hosaka, H Ishida
Mar Drugs, 2017-06-20;15(6):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
VEGF-production by CCR2-dependent macrophages contributes to laser-induced choroidal neovascularization.
Authors: Krause T, Alex A, Engel D, Kurts C, Eter N
PLoS ONE, 2014-04-08;9(4):e94313.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Flow Cytometry
Suppression of laser-induced choroidal neovascularization by a CCR3 antagonist.
Authors: Mizutani, Takeshi, Ashikari, Masayuki, Tokoro, Mayumi, Nozaki, Miho, Ogura, Yuichiro
Invest Ophthalmol Vis Sci, 2013-02-28;54(2):1564-72.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
Pericytes promote endothelial cell survival through induction of autocrine VEGF-A signaling and Bcl-w expression.
Authors: Franco M, Roswall P, Cortez E, Hanahan D, Pietras K
Blood, 2011-07-21;118(10):2906-17.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot