Home / VEGF / Mouse VEGF164 Antibody

Mouse VEGF164 Antibody

Catalog #: AF-493-NA
92 Citations 1 Review Datasheet / COA / SDS
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AF-493-NA
AF-493-SP
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Detection of Recombinant Human, Mouse, and Rat VEGF by Western Blot.
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse VEGF164 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse VEGF in ELISAs and Western blots. In Western blots, detection of recombinant mouse (rm) VEGF164, rmVEGF120, rmVEGF188, and recombinant rat VEGF164 is observered but no cross-reactivity with rmVEGF-B167,rmVEGF-B186 , or recombinant human (rh) VEGF165 is observed. In sandwich immunoassays, less than 0.04% cross-reactivity with rhVEGF121, rhVEGF165, and rhVEGF/PlGF is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse VEGF164
Ala27-Arg190
Accession # AAA40547
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Mouse VEGF Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Mouse VEGF Biotinylated Antibody (Catalog # BAF493)

Standard: Recombinant Mouse VEGF 164 Protein (Catalog # 493-MV)

Neutralization
Measured by its ability to neutralize VEGF164-induced proliferation in HUVEC human umbilical vein endothelial cells. Conn, G. et al. (1990) Proc. Natl. Acad. Sci USA 87:1323. The Neutralization Dose (ND50) is typically <0.15 µg/mL in the presence of 10 ng/mL Recombinant Mouse VEGF164.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Recombinant Human, Mouse, and Rat VEGF antibody by Western Blot. View Larger

Detection of Recombinant Human, Mouse, and Rat VEGF by Western Blot. Western blot shows 25 ng of Recombinant Human VEGF165(Catalog # 293-VE), Recombinant Mouse VEGF164(Catalog # 493-MV), Recombinant Mouse VEGF120(Catalog # 494-VE), Recombinant Mouse VEGF-B167(Catalog # 2595-VE), Recombinant Mouse VEGF-B 186 (Catalog # 767-VE), Recombinant Mouse VEGF188(Catalog # 7916-MV), and Recombinant Rat VEGF164(Catalog # 564-RV). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Mouse VEGF164Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-493-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for VEGF at approximately 18-27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Immunohistochemistry VEGF<sub>164</sub>in Mouse Kidney. View Larger

VEGF164in Mouse Kidney. Vascular Endothelial Growth Factor 164 (VEGF164) was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse VEGF164Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-493-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained (red) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Neutralization View Larger

Cell Proliferation Induced by VEGF164 and Neutralization by Mouse VEGF Antibody. Recombinant Mouse VEGF164 (Catalog # 493-MV) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line), as measured by Resazurin (AR002). Proliferation elicited by Recombinant Mouse VEGF164 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse VEGF164 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-493-NA). The ND50 is typically <0.15 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VEGF

Vascular Endothelial Growth Factor (VEGF or VEGF-A), also known as Vascular Permeability Factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues and a cystine knot structure. VEGF164 appears to be the most abundant and potent isoform, followed by VEGF120 and VEGF188. Mouse VEGF164 is an approximately 50 kDa molecular weight homodimer sharing 97% aa sequence identity with corresponding regions of rat, 89% with human and porcine, 90% with feline, equine and canine, and 88% with bovine VEGF, respectively. VEGF binds the type I transmembrane receptor tyrosine kinases VEGF R1 (also called Flt-1) and VEGF R2 (Flk-1/KDR) on endothelial cells. Although VEGF affinity is highest for binding to VEGF R1, VEGF R2 appears to be the primary mediator of VEGF angiogenic activity. Human VEGF165 binds the Semaphorin receptor, Neuropilin-1 and promotes complex formation with VEGF R2. VEGF is required during embryogenesis and functions to regulate the proliferation, migration, and survival of endothelial cells. In adults, VEGF functions mainly in wound healing and the female reproductive cycle. Pathologically, it is involved in tumor angiogenesis and vascular leakage. Circulating VEGF levels correlate with disease activity in autoimmune diseases such as rheumatoid arthritis, multiple sclerosis and systemic lupus erythematosus. VEGF is induced by hypoxia and cytokines such as IL-1, IL-6, IL-8, Oncostatin M (OSM) and TNF-alpha.

Long Name
Vascular Endothelial Growth Factor
Entrez Gene IDs
7422 (Human); 22339 (Mouse); 83785 (Rat); 281572 (Bovine); 403802 (Canine); 493845 (Feline); 30682 (Zebrafish)
Alternate Names
MVCD1; VAS; vascular endothelial growth factor A; Vascular permeability factor; Vasculotropin; VEGF; VEGFA; VEGF-A; VEGFMGC70609; VPF; VPFvascular endothelial growth factor

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Citations for Mouse VEGF164 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

92 Citations: Showing 1 - 10
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  1. The Ethyl Acetate Extract of Phyllanthus emblica L. Alleviates Diabetic Nephropathy in a Murine Model of Diabetes
    Authors: Lin, CH;Shih, CC;
    International journal of molecular sciences
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. A brainstem-hypothalamus neuronal circuit reduces feeding upon heat exposure
    Authors: Benevento, M;Alpár, A;Gundacker, A;Afjehi, L;Balueva, K;Hevesi, Z;Hanics, J;Rehman, S;Pollak, DD;Lubec, G;Wulff, P;Prevot, V;Horvath, TL;Harkany, T;
    Nature
    Species: Murine polyomavirus strain A3, Viral
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  3. Vertical Sleeve Gastrectomy Offers Protection against Disturbed Flow-Induced Atherosclerosis in High-Fat Diet-Fed Mice
    Authors: JH Wei, WJ Lee, JL Luo, HL Huang, SC Wang, RH Chou, PH Huang, SJ Lin
    International Journal of Molecular Sciences, 2023-03-16;24(6):.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  4. An agonistic anti-Tie2 antibody suppresses the normal-to-tumor vascular transition in the glioblastoma invasion zone
    Authors: E Lee, EA Lee, E Kong, H Chon, M Llaiqui-Co, CH Park, BY Park, NR Kang, JS Yoo, HS Lee, HS Kim, SH Park, SW Choi, D Vestweber, JH Lee, P Kim, WS Lee, I Kim
    Experimental & Molecular Medicine, 2023-02-24;55(2):470-484.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Kallistatin prevents ovarian hyperstimulation syndrome by regulating vascular leakage
    Authors: J Huang, Y Mao, Q Li, H Hong, N Tang, X Kang, Y Huang, J Liu, Q Gong, Y Yao, L Li
    Journal of Cellular and Molecular Medicine, 2022-07-21;26(16):4613-4623.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  6. Regenerating glomerular metabolism and function by podocyte pyruvate kinase M2 in diabetic nephropathy
    Authors: J Fu, T Shinjo, Q Li, R St-Louis, K Park, MG Yu, H Yokomizo, F Simao, Q Huang, IH Wu, GL King
    JCI Insight, 2022-03-08;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  7. Vascular endothelial growth factor-A/vascular endothelial growth factor2 signaling in spinal neurons contributes to bone cancer pain
    Authors: LJ Fan, HM Kan, XT Chen, YY Sun, LP Chen, W Shen
    Molecular pain, 2022-01-01;18(0):1744806922107.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. Efficient protein incorporation and release by a jigsaw-shaped self-assembling peptide hydrogel for injured brain regeneration
    Authors: A Yaguchi, M Oshikawa, G Watanabe, H Hiramatsu, N Uchida, C Hara, N Kaneko, K Sawamoto, T Muraoka, I Ajioka
    Nature Communications, 2021-11-19;12(1):6623.
    Species: Mouse
    Sample Types: Protein
    Applications: ELISA Capture
  9. VEGF Contributes to Mesenchymal Stem Cell-Mediated Reversion of Nor1-Dependent Hypertrophy in iPS Cell-Derived Cardiomyocytes
    Authors: D Philipp, M Holthaus, V Basoah, K Pfannkuche, L Suhr, T Wahlers, A Paunel-Gör
    Stem Cells International, 2021-04-10;2021(0):8888575.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: Neutralization, Western Blot
  10. PDGF Receptor Alpha Signaling Is Key for M�ller Cell Homeostasis Functions
    Authors: N Díaz-Lezam, A Wolf, S Koch, AM Pfaller, J Biber, X Guillonnea, T Langmann, A Grosche
    International Journal of Molecular Sciences, 2021-01-25;22(3):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  11. Cancer immunotherapy via targeted TGF-&beta signalling blockade in TH cells
    Authors: S Li, M Liu, MH Do, C Chou, EG Stamatiade, BG Nixon, W Shi, X Zhang, P Li, S Gao, KJ Capistrano, H Xu, NV Cheung, MO Li
    Nature, 2020-10-21;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  12. Osteocyte Vegf-a contributes to myeloma-associated angiogenesis and is regulated by Fgf23
    Authors: PL Mulcrone, SKE Edwards, DN Petrusca, LS Haneline, J Delgado-Ca, GD Roodman
    Sci Rep, 2020-10-14;10(1):17319.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  13. Overexpression of microRNA-367 inhibits angiogenesis in ovarian cancer by downregulating the expression of LPA1
    Authors: Q Zheng, X Dai, W Fang, Y Zheng, J Zhang, Y Liu, D Gu
    Cancer Cell Int, 2020-10-02;20(0):476.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  14. Activated FGF2 signaling pathway in tumor vasculature is essential for acquired resistance to anti-VEGF therapy
    Authors: K Ichikawa, S Watanabe M, Y Minoshima, J Matsui, Y Funahashi
    Sci Rep, 2020-02-19;10(1):2939.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  15. Chitinase-3-Like-1 Promotes M2 Macrophage Differentiation and Induces Choroidal Neovascularization in Neovascular Age-Related Macular Degeneration
    Authors: N Xu, Q Bo, R Shao, J Liang, Y Zhai, S Yang, F Wang, X Sun
    Invest. Ophthalmol. Vis. Sci., 2019-11-01;60(14):4596-4605.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  16. Sustained intraocular vascular endothelial growth factor neutralisation does not affect retinal and choroidal vasculature in Ins2Akita diabetic mice
    Authors: Judith Lechner, Jose R Hombrebueno, Edoardo Pedrini, Mei Chen, Heping Xu
    Diabetes and Vascular Disease Research
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  17. Serum miRNAs are potential biomarkers for the detection of disc degeneration, among which miR-26a-5p suppresses Smad1 to regulate disc homeostasis
    Authors: Y Fan, L Zhao, W Xie, D Yi, S He, D Chen, J Huang
    J. Cell. Mol. Med., 2019-07-23;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  18. Protease-activated receptor 2 protects against VEGF inhibitor-induced glomerular endothelial and podocyte injury
    Authors: Y Oe, T Fushima, E Sato, A Sekimoto, K Kisu, H Sato, J Sugawara, S Ito, N Takahashi
    Sci Rep, 2019-02-27;9(1):2986.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  19. Osteoblasts are "educated" by crosstalk with metastatic breast cancer cells in the bone tumor microenvironment
    Authors: AD Kolb, AB Shupp, D Mukhopadhy, FC Marini, KM Bussard
    Breast Cancer Res., 2019-02-27;21(1):31.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  20. Regulation of the bi-directional cross-talk between ovarian cancer cells and adipocytes by SPARC
    Authors: B John, C Naczki, C Patel, A Ghoneum, S Qasem, Z Salih, N Said
    Oncogene, 2019-02-14;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  21. Co-inhibition of PGF and VEGF blocks their expression in mononuclear phagocytes and limits neovascularization and leakage in the murine retina
    Authors: C Balser, A Wolf, M Herb, T Langmann
    J Neuroinflammation, 2019-02-07;16(1):26.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  22. Regeneration-associated cell transplantation contributes to tissue recovery in mice with acute ischemic stroke
    Authors: T Nakayama, E Nagata, H Masuda, T Asahara, S Takizawa
    PLoS ONE, 2019-01-25;14(1):e0210198.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  23. Bone Morphogenetic Protein 9 Regulates Early Lymphatic-Specified Endothelial Cell Expansion during Mouse Embryonic Stem Cell Differentiation
    Authors: M Subileau, G Merdzhanov, D Ciais, V Collin-Fau, JJ Feige, S Bailly, D Vittet
    Stem Cell Reports, 2018-12-27;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  24. The AB loop of oncostatin M (OSM) determines species-specific signaling in humans and mice
    Authors: JM Adrian-Seg, K Sreenivasa, P Gajawada, H Lörchner, T Braun, J Pöling
    J. Biol. Chem., 2018-10-29;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  25. Hapten-mediated recruitment of polyclonal antibodies to tumors engenders antitumor immunity
    Authors: B Schrand, E Clark, A Levay, AR Capote, O Martinez, R Brenneman, I Castro, E Gilboa
    Nat Commun, 2018-08-22;9(1):3348.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  26. Bushen Huoxue Recipe Alleviates Implantation Loss in Mice by Enhancing Estrogen-Progesterone Signals and Promoting Decidual Angiogenesis Through FGF2 During Early Pregnancy
    Authors: J Ding, X Tan, K Song, W Ma, J Xiao, Y Song, M Zhang
    Front Pharmacol, 2018-05-15;9(0):437.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  27. Interleukin-18 Amplifies Macrophage Polarization and Morphological Alteration, Leading to Excessive Angiogenesis
    Authors: T Kobori, S Hamasaki, A Kitaura, Y Yamazaki, T Nishinaka, A Niwa, S Nakao, H Wake, S Mori, T Yoshino, M Nishibori, H Takahashi
    Front Immunol, 2018-03-06;9(0):334.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  28. Vascularization via activation of VEGF-VEGFR signaling is essential for peripheral nerve regeneration
    Authors: Y Nishida, Y Yamada, H Kanemaru, A Ohazama, T Maeda, K Seo
    Biomed. Res., 2018-01-01;39(6):287-294.
    Species: Mouse
    Sample Types:
    Applications: Neutralization
  29. Modulation of Salmonella Tumor-Colonization and Intratumoral Anti-angiogenesis by Triptolide and Its Mechanism
    Authors: J Chen, Y Qiao, B Tang, G Chen, X Liu, B Yang, J Wei, X Zhang, X Cheng, P Du, W Jiang, Q Hu, ZC Hua
    Theranostics, 2017-06-01;7(8):2250-2260.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  30. Ferrochelatase is a therapeutic target for ocular neovascularization
    Authors: HD Basavaraja, RS Sulaiman, X Qi, T Shetty, S Sheik Pran, KL Sishtla, B Lee, J Quigley, S Alkhairy, CM Briggs, K Gupta, B Tang, M Shadmand, MB Grant, ME Boulton, SY Seo, TW Corson
    EMBO Mol Med, 2017-06-01;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Functional Assay
  31. Control of metastatic niche formation by targeting APBA3/Mint3 in inflammatory monocytes
    Authors: T Hara, HJ Nakaoka, T Hayashi, K Mimura, D Hoshino, M Inoue, F Nagamura, Y Murakami, M Seiki, T Sakamoto
    Proc. Natl. Acad. Sci. U.S.A., 2017-05-15;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  32. CYP4A in tumor-associated macrophages promotes pre-metastatic niche formation and metastasis
    Authors: XW Chen, TJ Yu, J Zhang, Y Li, HL Chen, GF Yang, W Yu, YZ Liu, XX Liu, CF Duan, HL Tang, M Qiu, CL Wang, H Zheng, J Yue, AM Guo, J Yang
    Oncogene, 2017-05-08;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Functional Assay
  33. VEGF as a Paracrine Regulator of Conventional Outflow Facility
    Authors: E Reina-Torr, JC Wen, KC Liu, G Li, JM Sherwood, JY Chang, P Challa, CM Flügel-Koc, WD Stamer, RR Allingham, DR Overby
    Invest. Ophthalmol. Vis. Sci., 2017-03-01;58(3):1899-1908.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  34. A delay in vascularization induces abnormal astrocyte proliferation and migration in the mouse retina
    Authors: Akane Morita
    Dev. Dyn, 2017-02-03;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  35. The biomechanical properties of an epithelial tissue determine the location of its vasculature
    Nat Commun, 2016-12-20;7(0):13560.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  36. PDGF-CC underlies resistance to VEGF-A inhibition and combinatorial targeting of both suppresses pathological angiogenesis more efficiently
    Authors: L Zheng, C Zhao, Y Du, X Lin, Y Jiang, C Lee, G Tian, J Mi, X Li, Q Chen, Z Ye, L Huang, S Wang, X Ren, L Xing, W Chen, D Huang, Z Gao, S Zhang, W Lu, Z Tang, B Wang, R Ju, X Li
    Oncotarget, 2016-11-22;7(47):77902-77915.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  37. Intrafibrillar silicified collagen scaffold modulates monocyte to promote cell homing, angiogenesis and bone regeneration
    Authors: Ji-Hua Chen
    Biomaterials, 2016-10-31;113(0):203-216.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  38. Retinal pigment epithelial cell expression of active Rap 1a by scAAV2 inhibits choroidal neovascularization
    Mol Ther Methods Clin Dev, 2016-08-24;3(0):16056.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  39. Morphine decreases the pro-angiogenic interaction between breast cancer cells and macrophages in vitro
    Sci Rep, 2016-08-12;6(0):31572.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  40. Antihypoxic Potentiation of Standard Therapy for Experimental Colorectal Liver Metastasis through Myo-Inositol Trispyrophosphate
    Clin Cancer Res, 2016-08-03;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  41. A novel small molecule ameliorates ocular neovascularisation and synergises with anti-VEGF therapy
    Sci Rep, 2016-05-05;6(0):25509.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  42. Endothelial Cords Promote Tumor Initial Growth prior to Vascular Function through a Paracrine Mechanism
    Authors: C Zhao, W Zhang, Y Zhao, Y Yang, H Luo, G Ji, E Dong, H Deng, S Lin, Y Wei, H Yang
    Sci Rep, 2016-01-14;6(0):19404.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  43. HMGB1 induces angiogenesis in rheumatoid arthritis via HIF-1alpha activation.
    Authors: Park S, Lee S, Kim H, Lee W, Hong K, Kim C
    Eur J Immunol, 2015-01-15;45(4):1216-27.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  44. Retinal neurons curb inflammation and enhance revascularization in ischemic retinopathies via proteinase-activated receptor-2.
    Authors: Sitaras N, Rivera J, Noueihed B, Bien-Aime M, Zaniolo K, Omri S, Hamel D, Zhu T, Hardy P, Sapieha P, Joyal J, Chemtob S
    Am J Pathol, 2014-12-03;185(2):581-95.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Neutralization
  45. Cessation of CCL2 inhibition accelerates breast cancer metastasis by promoting angiogenesis.
    Authors: Bonapace L, Coissieux M, Wyckoff J, Mertz K, Varga Z, Junt T, Bentires-Alj M
    Nature, 2014-10-22;515(7525):130-3.
    Species: Mouse
    Sample Types: In Vivo
  46. Breast cancer cells condition lymphatic endothelial cells within pre-metastatic niches to promote metastasis.
    Authors: Lee E, Fertig E, Jin K, Sukumar S, Pandey N, Popel A
    Nat Commun, 2014-09-02;5(0):4715.
    Species: Mouse
    Sample Types: In Vivo, Whole Tissue
    Applications: IHC, Neutralization
  47. Derivation and expansion of PAX7-positive muscle progenitors from human and mouse embryonic stem cells.
    Authors: Shelton M, Metz J, Liu J, Carpenedo R, Demers S, Stanford W, Skerjanc I
    Stem Cell Reports, 2014-08-07;3(3):516-29.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: IHC
  48. RhoA/phosphatidylinositol 3-kinase/protein kinase B/mitogen-activated protein kinase signaling after growth arrest-specific protein 6/mer receptor tyrosine kinase engagement promotes epithelial cell growth and wound repair via upregulation of hepatocyte growth factor in macrophages.
    Authors: Lee Y, Park H, Woo S, Park E, Kang J
    J Pharmacol Exp Ther, 2014-06-17;350(3):563-77.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  49. An inducible hepatocellular carcinoma model for preclinical evaluation of antiangiogenic therapy in adult mice.
    Authors: Runge A, Hu J, Wieland M, Bergeest J, Mogler C, Neumann A, Geraud C, Arnold B, Rohr K, Komljenovic D, Schirmacher P, Goerdt S, Augustin H
    Cancer Res, 2014-06-06;74(15):4157-69.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  50. Granzyme B releases vascular endothelial growth factor from extracellular matrix and induces vascular permeability.
    Authors: Hendel, Alon, Hsu, Ivy, Granville, David J
    Lab Invest, 2014-05-05;94(7):716-25.
    Species: Mouse
    Sample Types: In Vivo, Whole Tissue
    Applications: IHC, IHC-P, Neutralization
  51. Angiopoietin-1 suppresses choroidal neovascularization and vascular leakage.
    Authors: Lee, Junyeop, Park, Dae-Youn, Park, Do Young, Park, Intae, Chang, Woohyok, Nakaoka, Yoshikaz, Komuro, Issei, Yoo, Ook-Joon, Koh, Gou Youn
    Invest Ophthalmol Vis Sci, 2014-04-07;55(4):2191-9.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  52. Fatty acid-binding protein 5 (FABP5) regulates cognitive function both by decreasing anandamide levels and by activating the nuclear receptor peroxisome proliferator-activated receptor beta/delta (PPARbeta/delta) in the brain.
    Authors: Yu S, Levi L, Casadesus G, Kunos G, Noy N
    J Biol Chem, 2014-03-18;289(18):12748-58.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  53. Shiga toxin promotes podocyte injury in experimental hemolytic uremic syndrome via activation of the alternative pathway of complement.
    Authors: Locatelli M, Buelli S, Pezzotta A, Corna D, Perico L, Tomasoni S, Rottoli D, Rizzo P, Conti D, Thurman J, Remuzzi G, Zoja C, Morigi M
    J Am Soc Nephrol, 2014-02-27;25(8):1786-98.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  54. Dendritic cells: In vitro culture in two- and three-dimensional collagen systems and expression of collagen receptors in tumors and atherosclerotic microenvironments.
    Authors: Sprague L, Muccioli M, Pate M, Singh M, Xiong C, Ostermann A, Niese B, Li Y, Li Y, Courreges M, Benencia F
    Exp Cell Res, 2014-02-22;323(1):7-27.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development (Detection)
  55. IKK2 inhibition attenuates laser-induced choroidal neovascularization.
    Authors: Lu H, Lu Q, Gaddipati S, Kasetti R, Wang W, Pasparakis M, Kaplan H, Li Q
    PLoS ONE, 2014-01-28;9(1):e87530.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  56. Folic acid mitigates angiotensin-II-induced blood pressure and renal remodeling.
    Authors: Pushpakumar, Sathnur, Kundu, Sourav, Metreveli, Naira, Sen, Utpal
    PLoS ONE, 2013-12-26;8(12):e83813.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  57. Endothelial lineage differentiation from induced pluripotent stem cells is regulated by microRNA-21 and transforming growth factor beta2 (TGF-beta2) pathways.
    Authors: Di Bernardini E, Campagnolo P, Margariti A, Zampetaki A, Karamariti E, Hu Y, Xu Q
    J Biol Chem, 2013-12-19;289(6):3383-93.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  58. MicroRNA-210 overexpression induces angiogenesis and neurogenesis in the normal adult mouse brain.
    Authors: Zeng, L, He, X, Wang, Y, Tang, Y, Zheng, C, Cai, H, Liu, J, Wang, Y, Fu, Y, Yang, G-Y
    Gene Ther, 2013-10-24;21(1):37-43.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  59. Neutrophils contribute to inflammatory lymphangiogenesis by increasing VEGF-A bioavailability and secreting VEGF-D.
    Authors: Tan K, Chong S, Wong F, Evrard M, Tan S, Keeble J, Kemeny D, Ng L, Abastado J, Angeli V
    Blood, 2013-10-10;122(22):3666-77.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  60. IL-18 regulates melanoma VLA-4 integrin activation through a Hierarchized sequence of inflammatory factors.
    Authors: Valcarcel M, Carrascal T, Crende O, Vidal-Vanaclocha F
    J Invest Dermatol, 2013-08-12;134(2):470-80.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  61. G protein-coupled receptor kinase 6 deficiency promotes angiogenesis, tumor progression, and metastasis.
    Authors: Raghuwanshi S, Smith N, Rivers E, Thomas A, Sutton N, Hu Y, Mukhopadhyay S, Chen X, Leung T, Richardson R
    J Immunol, 2013-04-15;190(10):5329-36.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Development (Capture)
  62. The role of IL-1beta in the early tumor cell-induced angiogenic response.
    Authors: Carmi Y, Dotan S, Rider P, Kaplanov I, White M, Baron R, Abutbul S, Huszar M, Dinarello C, Apte R, Voronov E
    J Immunol, 2013-03-08;190(7):3500-9.
    Species: Mouse
    Sample Types: In Vivo, Matrigel Plug
    Applications: IHC-Fr, Neutralization
  63. Inhibition of PAI-1 induces neutrophil-driven neoangiogenesis and promotes tissue regeneration via production of angiocrine factors in mice.
    Blood, 2012-05-09;119(26):6382-93.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  64. Response patterns of cytokines/chemokines in two murine strains after irradiation.
    Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
    Cytokine, 2012-01-25;58(2):169-77.
    Species: Mouse
    Sample Types: Plasma
    Applications: Luminex Development
  65. Relationship between complement membrane attack complex, chemokine (C-C motif) ligand 2 (CCL2) and vascular endothelial growth factor in mouse model of laser-induced choroidal neovascularization.
    Authors: Liu J, Jha P, Lyzogubov VV, Tytarenko RG, Bora NS, Bora PS
    J. Biol. Chem., 2011-04-22;286(23):20991-1001.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  66. IL-17 and VEGF are necessary for efficient corneal nerve regeneration.
    Authors: Li Z, Burns AR, Han L, Rumbaut RE, Smith CW
    Am. J. Pathol., 2011-03-01;178(3):1106-16.
    Species: Mouse
    Sample Types: In Vivo, Whole Tissue
    Applications: IHC-Fr, Neutralization
  67. Serotonin reverts age-related capillarization and failure of regeneration in the liver through a VEGF-dependent pathway.
    Authors: Furrer K, Rickenbacher A, Tian Y, Jochum W, Bittermann AG, Kach A, Humar B, Graf R, Moritz W, Clavien PA
    Proc. Natl. Acad. Sci. U.S.A., 2011-01-31;108(7):2945-50.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  68. CXCR4 blockade augments bone marrow progenitor cell recruitment to the neovasculature and reduces mortality after myocardial infarction.
    Authors: Jujo K, Hamada H, Iwakura A, Thorne T, Sekiguchi H, Clarke T, Ito A, Misener S, Tanaka T, Klyachko E, Kobayashi K, Tongers J, Roncalli J, Tsurumi Y, Hagiwara N, Losordo DW
    Proc. Natl. Acad. Sci. U.S.A., 2010-06-01;107(24):11008-13.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  69. Platelet-derived growth factor-B normalizes micromorphology and vessel function in vascular endothelial growth factor-A-induced squamous cell carcinomas.
    Authors: Lederle W, Linde N, Heusel J, Bzyl J, Woenne EC, Zwick S, Skobe M, Kiessling F, Fusenig NE, Mueller MM
    Am. J. Pathol., 2009-12-30;176(2):981-94.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  70. VEGF-A expression by HSV-1-infected cells drives corneal lymphangiogenesis.
    Authors: Wuest TR, Carr DJ
    J. Exp. Med., 2009-12-21;207(1):101-15, S1-2.
    Species: Mouse
    Sample Types: In Vivo, Whole Tissue
    Applications: IHC, Neutralization
  71. Effects of increased renal tubular vascular endothelial growth factor (VEGF) on fibrosis, cyst formation, and glomerular disease.
    Authors: Hakroush S, Moeller MJ, Theilig F, Kaissling B, Sijmonsma TP, Jugold M, Akeson AL, Traykova-Brauch M, Hosser H, Hahnel B, Grone HJ, Koesters R, Kriz W
    Am. J. Pathol., 2009-10-15;175(5):1883-95.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  72. Critical role of CD11b+ macrophages and VEGF in inflammatory lymphangiogenesis, antigen clearance, and inflammation resolution.
    Authors: Kataru RP, Jung K, Jang C, Yang H, Schwendener RA, Baik JE, Han SH, Alitalo K, Koh GY
    Blood, 2009-04-03;113(22):5650-9.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  73. Recapitulation of pancreatic neuroendocrine tumors in human multiple endocrine neoplasia type I syndrome via Pdx1-directed inactivation of Men1.
    Authors: Shen HC, He M, Powell A, Adem A, Lorang D, Heller C, Grover AC, Ylaya K, Hewitt SM, Marx SJ, Spiegel AM, Libutti SK
    Cancer Res., 2009-02-10;69(5):1858-66.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr, IHC-P
  74. Safety profile of topical VEGF neutralization at the cornea.
    Authors: Bock F, Onderka J, Rummelt C, Dietrich T, Bachmann B, Kruse FE, Schlotzer-Schrehardt U, Cursiefen C
    Invest. Ophthalmol. Vis. Sci., 2009-01-17;50(5):2095-102.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Blocking
  75. Modulation of angiogenesis by a tetrameric tripeptide that antagonizes vascular endothelial growth factor receptor 1.
    Authors: Ponticelli S, Marasco D, Tarallo V, Albuquerque RJ, Mitola S, Takeda A, Stassen JM, Presta M, Ambati J, Ruvo M, De Falco S
    J. Biol. Chem., 2008-10-15;283(49):34250-9.
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: ELISA Development
  76. Fibroblast-type reticular stromal cells regulate the lymph node vasculature.
    Authors: Chyou S, Ekland EH, Carpenter AC, Tzeng TC, Tian S, Michaud M, Madri JA, Lu TT
    J. Immunol., 2008-09-15;181(6):3887-96.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  77. Role of cell and matrix-bound VEGF isoforms in lens development.
    Authors: Saint-Geniez M, Kurihara T, D'Amore PA
    Invest. Ophthalmol. Vis. Sci., 2008-08-29;50(1):311-21.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  78. Regulation of scar formation by vascular endothelial growth factor.
    Authors: Wilgus TA, Ferreira AM, Oberyszyn TM, Bergdall VK, DiPietro LA
    Lab. Invest., 2008-04-21;88(6):579-90.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  79. Loss of vascular endothelial growth factor expression reduces vascularization, but not growth, of tumors lacking the Von Hippel-Lindau tumor suppressor gene.
    Authors: Blouw B, Haase VH, Song H, Bergers G, Johnson RS
    Oncogene, 2007-02-05;26(31):4531-40.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  80. Platelet-derived growth factor-BB controls epithelial tumor phenotype by differential growth factor regulation in stromal cells.
    Authors: Lederle W, Stark HJ, Skobe M, Fusenig NE, Mueller MM
    Am. J. Pathol., 2006-11-01;169(5):1767-83.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  81. Vascular endothelial growth factor-A mediates ultraviolet B-induced impairment of lymphatic vessel function.
    Authors: Kajiya K, Hirakawa S, Detmar M
    Am. J. Pathol., 2006-10-01;169(4):1496-503.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  82. The Myc-dependent angiogenic switch in tumors is mediated by interleukin 1beta.
    Authors: Shchors K, Shchors E, Rostker F, Lawlor ER, Brown-Swigart L, Evan GI
    Genes Dev., 2006-09-15;20(18):2527-38.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  83. Mast cells facilitate local VEGF release as an early event in the pathogenesis of postoperative peritoneal adhesions.
    Authors: Cahill RA, Wang JH, Soohkai S, Redmond HP
    Surgery, 2006-07-01;140(1):108-12.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  84. VEGF-C is a trophic factor for neural progenitors in the vertebrate embryonic brain.
    Authors: Le Bras B, Barallobre MJ, Homman-Ludiye J, Ny A, Wyns S, Tammela T, Haiko P, Karkkainen MJ, Yuan L, Muriel MP, Chatzopoulou E, Breant C, Zalc B, Carmeliet P, Alitalo K, Eichmann A, Thomas JL
    Nat. Neurosci., 2006-02-05;9(3):340-8.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  85. Loss of SPARC-mediated VEGFR-1 suppression after injury reveals a novel antiangiogenic activity of VEGF-A.
    Authors: Nozaki M, Sakurai E, Raisler BJ, Baffi JZ, Witta J, Ogura Y, Brekken RA, Sage EH, Ambati BK, Ambati J
    J. Clin. Invest., 2006-02-01;116(2):422-9.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  86. Application of plasmid DNA encoding IL-18 diminishes development of herpetic stromal keratitis by antiangiogenic effects.
    Authors: Kim B, Lee S, Suvas S, Rouse BT
    J. Immunol., 2005-07-01;175(1):509-16.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ELISA Development
  87. Differential roles of vascular endothelial growth factor receptors 1 and 2 in dendritic cell differentiation.
    Authors: Dikov MM, Ohm JE, Ray N, Tchekneva EE, Burlison J, Moghanaki D, Nadaf S, Carbone DP
    J. Immunol., 2005-01-01;174(1):215-22.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Neutralization
  88. Vascular endothelial growth factor (VEGF) gene transfer enhances surgical revascularization of necrotic bone.
    Authors: Katsube K, Bishop AT, Simari RD, Yla-Herttuala S, Friedrich PF
    J. Orthop. Res., 2004-11-30;23(2):469-74.
    Species: Rabbit
    Sample Types: Whole Tissue
    Applications: IHC
  89. CXCR2-/- mice show enhanced susceptibility to herpetic stromal keratitis: a role for IL-6-induced neovascularization.
    Authors: Banerjee K, Biswas PS, Kim B, Lee S, Rouse BT
    J. Immunol., 2004-01-15;172(2):1237-45.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  90. Estrogen regulates the production of VEGF for osteoclast formation and activity in op/op mice.
    Authors: Kodama I, Sanada M, Yoshiko Y, Tsuda M, Ohama K
    J. Bone Miner. Res., 2003-12-16;19(2):200-6.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  91. Lack of alpha2-antiplasmin promotes pulmonary heart failure via overrelease of VEGF after acute myocardial infarction.
    Authors: Kozawa O, Yoshimi N, Akamatsu S, Hara A, Mori H, Uematsu T
    Blood, 2002-10-01;100(7):2487-93.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  92. Antibodies to vascular endothelial growth factor enhance the efficacy of cancer immunotherapy by improving endogenous dendritic cell function.
    Authors: Gabrilovich DI, 2018, Ishida T, Nadaf S, Ohm JE, Carbone DP
    1537, 1999-10-01;5(10):2963-70.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization

FAQs

  1. What band size(s) are detected when using AF-493-NA for Western blot?

    • This antibody detects Recombinant Mouse VEGF164 at 42 kDa under non-reducing conditions and 21 kDa under reducing conditions.  Band sizes with natural samples may vary slightly depending on any post-translational modifications.  

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Mouse VEGF164 Antibody
By Leslie Priddy on 04/01/2018
Application: WB Sample Tested: Tumor cell lyastes Species: Mouse