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Mouse VEGF-D Antibody

Catalog #: MAB469
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB469
MAB469-SP
Product Details
Citations (2)
FAQs
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse VEGF-D Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse VEGF-D in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse (rm) VEGF165, rmVEGF-B186, recombinant human (rh) VEGF-C, or rhVEGF-D is observed.
Source
Monoclonal Rat IgG2A Clone # 90409
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse VEGF-D
Phe98-Ser206
Accession # P97946
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Purity
Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse VEGF-D (Catalog # 469-VD)

Mouse VEGF-D Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Mouse VEGF‑D Biotinylated Antibody (Catalog # BAF469)

Standard: Recombinant Mouse VEGF-D Protein (Catalog # 469-VD)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VEGF-D

Vascular endothelia growth factor D (VEGF-D), also known as c-fos-induced growth factor (FIGF), is a secreted glycoprotein of the VEGF/PDGF family. VEGFs regulate angiogenesis and lymphangiogenesis during development and tumor growth, and are characterized by eight conserved cysteine residues that form a cysteine-knot structure (1‑3). VEGF-C and VEGF-D, which share 23% amino acid (aa) sequence identity, are uniquely expressed as preproproteins that contain long N- and C-terminal propeptide extensions around the VEGF homology domain (VHD) (1, 2). Proteolytic processing of either 358 aa or 326 aa splice forms of mouse VEGF-D preproprotein creates a secreted proprotein. Further processing by extracellular serine proteases, such as plasmin or furin-like proprotein convertases, forms mature VEGF-D consisting of non-covalently linked 42 kDa homodimers of the 117 aa VHD (4‑7). Mature mouse VEGF-D shares 94%, 99%, 93%, 91% and 89% aa identity with the VHD of human, rat, equine, canine and bovine VEGF-D, respectively. It is expressed in adult lung, heart, muscle, and small intestine, and is most abundantly expressed in fetal lungs and skin (1 - 4). Mouse and human VEGF-D are ligands for VEGF receptor 3 (VEGF-R3, also called Flt-4) that are active across species and show enhanced affinity when processed (8). Unlike human VEGF-D, which is also a ligand for VEGF-R2 (also called Flk-1 or KDR), mouse VEGF-D does not bind to VEGF-R2 (8). VEGF-R3 is strongly expressed in lymphatic endothelial cells and is essential for regulation of the growth and differentiation of lymphatic endothelium (1, 2). While VEGF-C is the critical ligand for VEGF-R3 during embryonic lymphatic development, VEGF-D is most active in neonatal lymphatic maturation and bone growth (9‑11). Both promote tumor lymphangiogenesis (12). Consonant with their activity on VEGF receptors, binding of VEGF-C and VEGF-D to neuropilins contributes to VEGF-R3 signaling in lymphangiogenesis, while binding to integrin alpha 9 beta 1 mediates endothelial cell adhesion and migration (13, 14).

References
  1. Roy, H. et al. (2006) FEBS Lett. 580:2879.
  2. Otrock, Z.H. et al. (2007) Blood Cells Mol. Dis. 38:258.
  3. Orlandini, M. et al. (1996) Proc. Natl. Acad. Sci. USA 93:11675.
  4. Stacker, S.A. et al. (1999) J. Biol. Chem. 274:32127.
  5. McColl, B.K. et al. (2003) J. Exp. Med. 198:863.
  6. McColl, B.K. et al. (2007) FASEB J. 21:1088.
  7. Baldwin, M.E. et al. (2001) J. Biol. Chem. 276:44307.
  8. Baldwin, M.E. et al. (2001) J. Biol. Chem. 276:19166.
  9. Baldwin, M.E. et al. (2005) Mol. Cell. Biol. 25:2441.
  10. Karpanen, T. et al. (2006) Am. J. Pathol. 169:708.
  11. Orlandini, M. et al. (2006) J. Biol. Chem. 281:17961.
  12. Stacker, S.A. et al. (2001) Nature Med. 7:186.
  13. Karpanen, T. et al. (2006) FASEB J. 20:1462.
  14. Vlahakis, N.E. et al. (2005) J. Biol. Chem. 280:4544.
Long Name
Vascular Endothelial Growth Factor D/cFos-induced Growth Factor
Entrez Gene IDs
2277 (Human); 14205 (Mouse)
Alternate Names
c-fos induced growth factor (vascular endothelial growth factor D); FIGF; vascular endothelial growth factor D; VEGFD; VEGF-D; VEGF-DVEGFDc-Fos-induced growth factor

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Citations for Mouse VEGF-D Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. VEGF-D-induced draining lymphatic enlargement and tumor lymphangiogenesis promote lymph node metastasis in a xenograft model of ovarian carcinoma.
    Authors: Du, Li-Cheng, Chen, Xian-Che, Wang, Dong, Wen, Yan-Jun, Wang, Chun-Tin, Wang, Xue-Mei, Kan, Bing, Wei, Yu-Quan, Zhao, Xia
    Reprod Biol Endocrinol, 2014-02-06;12(0):14.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  2. A tumor-selective biotherapy with prolonged impact on established metastases based on cytokine gene-engineered MSCs.
    Authors: Chen X, Lin X, Zhao J, Shi W, Zhang H, Wang Y, Kan B, DU L, Wang B, Wei Y, Liu Y, Zhao X
    Mol. Ther., 2008-02-05;16(4):749-56.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P

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