Mouse VEGFR1/Flt-1 Antibody

Catalog #: MAB4711
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4711
MAB4711-SP

All VEGFR1/Flt-1 Antibodies

Product Details

Citations (2)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse VEGFR1/Flt-1 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse VEGFR1/Flt‑1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human VEGFR1, recombinant mouse (rm) VEGFR2, or rmVEGFR3 is observed.

Source

Monoclonal Rat IgG_2B Clone # 141522

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse VEGFR1/Flt‑1
Ser27-Glu759
Accession # P35969

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse VEGFR1/Flt‑1 Fc Chimera (Catalog # 471-F1)

Flow Cytometry

2.5 µg/10⁶ cells

bEnd.3 mouse endothelioma cell line

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VEGFR1/Flt-1

VEGFR1 is one of the five receptor tyrosine kinases (RTKs) (VEGFR1, KDR/Flk-1, Flt-4, Tie-1, and Tek/Tie-2) whose expression is almost exclusively restricted to the endothelial cells. Tie-1 and tek/tie-2 define a new class of RTKs containing two immunoglobulin-like domains, three EGF homology domains and three fibronectin type III domains in their extracellular regions. VEGFR1/Flt-1, VEGFR2/KDR/Flk-1, VEGFR3/Flt-4 are members of the class III subfamily of RTKs containing seven immunoglobulin-like repeats in their extracellular domains. All five RTKs are likely to play central roles in vasculogenesis and angiogenesis.

Full length mouse VEGFR1 mRNA encodes a 1333 amino acid (aa) residue precursor with a predicted 22 aa residue signal peptide. Mature VEGFR1 is composed of a 737 aa residue extracellular domain, a 22 aa residue transmembrane domain and a 552 aa residue cytoplasmic domain. As a result of alternative splicing of the mRNA, a cDNA encoding a truncated form of VEGFR1, lacking the seventh immunoglobulin-like domain, the transmembrane and intracellular domains, has been cloned. The recombinant soluble VEGFR1/Fc chimera binds VEGF and PlGF with high affinity and is a potent VEGF antagonist.

References

He, Y. et al. (1999) Molecular Endocrinology 13:537.
Ferrara, N. and T. Davis-Smyth (1997) Endocrine Reviews 8:4.

Long Name

Vascular Endothelial Growth Factor Receptor 1

Entrez Gene IDs

2321 (Human); 14254 (Mouse)

Alternate Names

EC 2.7.10; EC 2.7.10.1; FLT; FLT1; Flt-1; Fms-like tyrosine kinase 1; fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascularpermeability factor receptor); FRT; Tyrosine-protein kinase FRT; Tyrosine-protein kinase receptor FLT; vascular endothelial growth factor receptor 1; Vascular permeability factor receptor; VEGF R1; VEGFR1; VEGFR-1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse VEGFR1/Flt-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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VEGF-A modulates expression of inhibitory checkpoints on CD8+ T cells in tumors.
Authors: Voron T, Colussi O, Marcheteau E, Pernot S, Nizard M, Pointet A, Latreche S, Bergaya S, Benhamouda N, Tanchot C, Stockmann C, Combe P, Berger A, Zinzindohoue F, Yagita H, Tartour E, Taieb J, Terme M
J Exp Med, 2015-01-19;212(2):139-48.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry, Neutralization
Vascular endothelial growth factor can signal through platelet-derived growth factor receptors.
Authors: Ball SG, Shuttleworth CA, Kielty CM
J. Cell Biol., 2007-04-30;177(3):489-500.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry