Mouse VISTA/B7-H5/PD-1H APC-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB7005A
Detection of VISTA/B7-H5/PD-1H in Mouse Splenocytes by Flow Cytometry.
2 Images
Product Details
FAQs
Supplemental Products
Reviews

Mouse VISTA/B7-H5/PD-1H APC-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse VISTA/B7-H5/PD-1H in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human VISTA/B7-H5/PD-1H is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse VISTA/B7-H5/PD-1H
Phe33-Ala191
Accession # Q9D659
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of VISTA/B7-H5/PD-1H antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of VISTA/B7-H5/PD-1H in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB554P) and either (A) Sheep Anti-Mouse VISTA/B7-H5/PD-1H APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB7005A) or (B) Normal Sheep IgG Allophycocyanin Control (Catalog # IC016A). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of VISTA/B7-H5/PD-1H antibody in HEK293 Human Cell Line Transfected with Mouse VISTA and eGFP antibody by Flow Cytometry. View Larger

Detection of VISTA/B7-H5/PD-1H in HEK293 Human Cell Line Transfected with Mouse VISTA and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) mouse VISTA or (B) irrelevant transfectants and eGFP was stained with Sheep Anti-Mouse VISTA/B7-H5/PD-1H APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB7005A). Quadrant markers were set based on control antibody staining (Catalog # IC016A). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: VISTA/B7-H5/PD-1H

VISTA, also known as B7-H5, PD-1H, Platelet Receptor Gi24, Dies1 (Differentiation of ESC-1), SISP1 and C10orf54, is a 55-65 kDa member of the Ig superfamily. It is a transmembrane molecule expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces. On ESCs, VISTA appears to positively interact with BMP4, potentiating BMP signaling and the transition from an undifferentiated to a differentiated state. On tumor cells, VISTA both promotes MT1-MMP expression and activity, and serves as a substrate for MT1-MMP. This increases the potential for cell motility. Mature mouse VISTA is a 277 amino acid (aa) type I transmembrane glycoprotein (aa 33-309). It contains a 149 aa extracellular region (aa 33-191) with one V-type Ig-like domain (aa 33-161) and a 97 aa cytoplasmic domain. Based on human VISTA, mouse Gi24 will likely undergo proteolytic cleavage by MT1-MMP, generating a soluble 30 kDa extracellular fragment, plus a 25-30 kDa membrane-bound fragment. There are two potential isoform variants. One contains a deletion of aa 127-187, while another shows an alternative start site at Met82. Over aa 33-191, mouse VISTA/Dies1 shares 78% and 70% aa sequence identity with rat and human VISTA, respectively.

Entrez Gene IDs
64115 (Human); 74048 (Mouse); 690899 (Rat); 102116468 (Cynomolgus Monkey)
Alternate Names
4632428N05Rik; B7H5; B7-H5; C10orf54; chromosome 10 open reading frame 54; Dies1; Gi24; PD1H; PD-1H; platelet receptor Gi24; PP2135; SISP1; stress induced secreted protein 1; VISTA; VSIR

Product Datasheets

You must select a language.

x

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Mouse VISTA/B7-H5/PD-1H APC-conjugated Antibody

There are currently no reviews for this product. Be the first to review Mouse VISTA/B7-H5/PD-1H APC-conjugated Antibody and earn rewards!

Have you used Mouse VISTA/B7-H5/PD-1H APC-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review