Mouse Wnt-1 Antibody

Catalog #: AF1620
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF1620
AF1620-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Wnt-1 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Wnt-1 in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with recombinant mouse (rm) Wnt‑3a and rmWnt‑5a is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse Wnt‑1 peptide
Ser52-Glu92 and Met234-Arg313
Accession # Q3UR96

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Wnt‑1

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Wnt‑1 in Mouse Embryonic Spinal Cord. Wnt-1 was detected in immersion fixed frozen sections of mouse embryonic spinal cord (13 d.p.c.) using 15 µg/mL Goat Anti-Mouse Wnt-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1620) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Wnt-1

Wnt-1 is a secreted glycoprotein that binds members of the frizzled family of seven transmembrane domain receptors. Wnt-1 may have an important role in the morphogenesis of the neural tube and in CNS development.

Long Name

Wingless-type MMTV Integration Site Family, Member 1

Entrez Gene IDs

7471 (Human); 22408 (Mouse)

Alternate Names

Int-1; INT1Proto-oncogene Int-1 homolog; proto-oncogene Wnt-1; wingless-type MMTV integration site family, member 1 (oncogene INT1); wingless-type MMTV integration site family, member 1; Wnt1; Wnt-1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Mouse Wnt-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1