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Mouse Wnt-10b Antibody

Catalog #: MAB2110
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2110
MAB2110-SP
Product Details
Citations (3)
FAQs
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Wnt-10b Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Wnt-10b in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse Wnt-1, -3a, -4,
-5a, -5b, -8a, -11, or -16 is observed.
Source
Monoclonal Rat IgG2B Clone # 254206
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse Wnt-10b
Arg263-Arg332
Accession # P48614
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse Wnt‑10b

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Wnt-10b

Wnt-10b, also known as Wnt-12, is a 45 kDa glycoprotein involved in fetal limb bud development and adult hematopoiesis. Mouse Wnt-10b exists in two isoforms that are distinguished by the presence or absence of an internal 95 amino acid sequence.

Long Name
Wingless-type MMTV Integration Site Family, Member 10B
Entrez Gene IDs
7480 (Human); 22410 (Mouse)
Alternate Names
protein Wnt-10b; Protein Wnt-12; SHFM6WNT-10B protein; wingless-type MMTV integration site family, member 10B; Wnt10b; Wnt-10b; WNT12; Wnt-12

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Citations for Mouse Wnt-10b Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Wnt10b activates the wnt, notch, and NF kappa B pathways in u2os osteosarcoma cells
    Authors: Ulrike I. Mödder, Merry Jo Oursler, Sundeep Khosla, David G. Monroe
    Journal of Cellular Biochemistry
  2. Regulation of cyclin D1 and Wnt10b gene expression by cAMP-responsive element-binding protein during early adipogenesis involves differential promoter methylation.
    Authors: Fox KE, Colton LA, Erickson PF, Friedman JE, Cha HC, Keller P, MacDougald OA, Klemm DJ
    J. Biol. Chem., 2008-10-27;283(50):35096-105.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Msx2 exerts bone anabolism via canonical Wnt signaling.
    Authors: Cheng SL, Shao JS, Cai J, Sierra OL, Towler DA
    J. Biol. Chem., 2008-05-15;283(29):20505-22.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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