Mouse Wnt-2b Biotinylated Antibody

Catalog #: BAF3900 Datasheet / COA / SDS

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BAF3900

All Wnt-2b Antibodies

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Mouse Wnt-2b Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Wnt-2b in Western blots. In Western blots, approximately 25% cross-reactivity with recombinant human (rh) Wnt-2 is observed and less than 5% cross-reactivity with rhWnt-7a, rhWnt-9a, recombinant mouse (rm) Wnt-1, rmWnt-3a, rmWnt-4, rmWnt-5a, rmWnt-5b, rmWnt-8a, rmWnt-8b, rmWnt-9b, rmWnt-10a, rmWnt-10b, rmWnt-11, and rmWnt-16 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse Wnt‑2b
Leu64-Glu104 and Arg251-Ala312
Accession # O70283

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Wnt‑2b

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Wnt-2b

Mouse Wnt-2b (also Wnt-13) is a 389 amino acid, 42kDa member of the large and highly conserved Wnt family of secreted, cysteine-rich glycoprotein signaling molecules. Wnt proteins are associated with developmental and carcinogenic processes. Wnt-2b may be necessary to maintain stem cells in an undifferentiated state. As a type II transmembrane protein with a long C-terminal extracellular and a short N-terminal cytoplasmic domain, Wnt-2b is presumably cleaved within its extracellular region to release the secreted form. In the region from aa 64‑104, mouse Wnt-2b is 100% identical to the human and rat protein, and over the 251‑312 aa region also used as immunogen, mouse Wnt-2b is 97% and 100% identical to the corresponding human and rat sequences, respectively.

Long Name

Wingless-type MMTV Integration Site Family Member 2b

Entrez Gene IDs

7482 (Human); 22414 (Mouse); 116466 (Rat)

Alternate Names

Protein Wnt-13; protein Wnt-2b; wingless-type MMTV integration site family, member 2B; Wnt-13; WNT13XWNT2, Xenopus, homolog of; Wnt2b; Wnt-2b; XWNT2; XWNT2wingless-type MMTV integration site family, member 13