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Mouse Wnt-4 Antibody

Catalog #: AF475
12 Citations Datasheet / COA / SDS
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AF475
AF475-SP
Product Details
Citations (12)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Wnt-4 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Wnt-4 in direct ELISAs and Western blots. The antibody has also been shown to recognize a mammalian cell-expressed mouse Wnt-4 protein in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse Wnt-4
Ile37-Glu76, Arg222-Thr295
Accession # P22724
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Wnt‑4
Immunohistochemistry
5-15 µg/mL
Immersion fixed paraffin-embedded sections of rat embryo (E15)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Wnt-4

Wnt-4 is a 38‑42 kDa member of the Wnt family of secreted glycoproteins, which act as short-range signaling molecules via Frizzled receptors and a cascade of intracellular signals in vertebrate embryogenesis (1‑2). Mouse Wnt-4 is synthesized as a 351 amino acid (aa) precursor with a 22 aa signal sequence and a 329 aa mature chain. The mature chain contains two potential sites for N-linked glycosylation. Relative to other members of the Wnt family, Wnt-4 contains 83 conserved aa, including 21 cysteines (1). Mature mouse Wnt-4 shares 99% aa sequence identity with mature human and rat Wnt-4. Wnt-4 has been shown to play a critical role in the development of the reproductive system and in the formation of the kidneys, adrenals, pituitary gland, and mammary tissues (3‑6). In the development of the reproductive system, Wnt-4 expression is down-regulated in the developing gonad after E11.5, although it persists in the developing ovary (2, 6). Targeted deletion of Wnt-4 results in masculinization of XX mice, with rudimentary development of the masculine internal (Wolffian) ducts and degeneration of the female (Mullerian) reproductive tract (2, 6). In addition to its involvement in urogenital development, Wnt-4 is also expressed in the perichondrium of the long bones (7), and promotes osteoblast differentiation (8). Wnt-4 may also be associated with abnormal proliferation in human breast tissue (9). In humans, mutations in Wnt-4 are the cause of SERKAL syndrome, a syndrome consisting of female to male sex reversal, renal, adrenal, and lung dysgenesis, and developmental defects (3), and Rokitansky-Kuster-Hauser syndrome, which is characterized by utero-vaginal atresia in otherwise phenotypically normal females with normal 46, XX karyotype (10).

References
  1. Gavin, B.J. et al. (1990) Genes Dev. 4:2319.
  2. Jordan, B.K. et al. (2001) Am. J. Hum. Genet. 68:1102.
  3. Mandel, H. et al. (2008) Am. J. Hum. Genet. 82:39.
  4. Bernard, P. and V.R. Harley (2007) Int. J. Biochem. Cell Biol. 39:31.
  5. Kuulasmaa, T. et al. (2008) Horm. Metab. Res. 40:668.
  6. Vainio, S. et al. (1999) Nature 397:405.
  7. Gao, X. (2004) Genes Dev. 18:2404.
  8. Chang, J. et al. (2007) J. Biol. Chem. 282:30938.
  9. Huguet, E.L. et al. (1994) Cancer Res. 54:2615.
  10. Philibert, P. et al. (2008) J. Clin. Endocrinol. Metab. 93:895.
Long Name
Wingless-type MMTV Integration Site Family, Member 4
Entrez Gene IDs
54361 (Human); 22417 (Mouse)
Alternate Names
protein Wnt-4; SERKAL; wingless-type MMTV integration site family, member 4; Wnt4; Wnt-4

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Citations for Mouse Wnt-4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Acetyl-CoA is a key molecule for nephron progenitor cell pool maintenance
    Authors: Diniz, F;Ngo, NYN;Colon-Leyva, M;Edgington-Giordano, F;Hilliard, S;Zwezdaryk, K;Liu, J;El-Dahr, SS;Tortelote, GG;
    Nature communications
    Species: Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Wnt4 is heterogeneously activated in maturing beta-cells to control calcium signaling, metabolism and function
    Authors: K Katsumoto, S Yennek, C Chen, LFD Silva, S Traikov, D Sever, A Azad, J Shan, S Vainio, N Ninov, S Speier, A Grapin-Bot
    Nature Communications, 2022-10-21;13(1):6255.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Macrophages promote epithelial proliferation following infectious and non-infectious lung injury through a Trefoil factor 2-dependent mechanism
    Authors: LY Hung, D Sen, TK Oniskey, J Katzen, NA Cohen, AE Vaughan, W Nieves, A Urisman, MF Beers, MF Krummel, DR Herbert
    Mucosal Immunol, 2018-10-18;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  4. GLI1-expressing mesenchymal cells form the essential Wnt-secreting niche for colon stem cells
    Authors: B Degirmenci, T Valenta, S Dimitrieva, G Hausmann, K Basler
    Nature, 2018-06-06;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Identification of proliferative and mature ?-cells in the islets of Langerhans
    Nature, 2016-07-11;535(7612):430-4.
    Species: Human
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC-P, Western Blot
  6. WINGLESS (WNT) signaling is a progesterone target for rat uterine stromal cell proliferation
    Authors: Bruce F Kimler
    J. Endocrinol., 2016-03-14;229(2):197-207.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-P
  7. Lgr5 marks stem/progenitor cells in ovary and tubal epithelia.
    Authors: Ng A, Tan S, Singh G, Rizk P, Swathi Y, Tan T, Huang R, Leushacke M, Barker N
    Nat Cell Biol, 2014-07-06;16(8):745-57.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. Wnt4/ beta −Catenin Signaling in Medullary Kidney Myofibroblasts
    Authors: Derek P. DiRocco, Akio Kobayashi, Makoto M. Taketo, Andrew P. McMahon, Benjamin D. Humphreys
    Journal of the American Society of Nephrology
  9. Habenular commissure formation in zebrafish is regulated by the pineal gland-specific gene unc119c
    Authors: Reiko Toyama, Mi Ha Kim, Martha L. Rebbert, John Gonzales, Harold Burgess, Igor B. Dawid
    Developmental Dynamics
  10. Wnt4 Enhances Murine Hematopoietic Progenitor Cell Expansion Through a Planar Cell Polarity-Like Pathway
    Authors: Krista M. Heinonen, Juan Ruiz Vanegas, Deborah Lew, Jana Krosl, Claude Perreault
    PLoS ONE
  11. Dickkopf-1 is a master regulator of joint remodeling.
    Authors: Diarra D, Stolina M, Polzer K, Zwerina J, Ominsky MS, Dwyer D, Korb A, Smolen J, Hoffmann M, Scheinecker C, van der Heide D, Landewe R, Lacey D, Richards WG, Schett G
    Nat. Med., 2007-01-21;13(2):156-63.
    Species: Human, Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-P, Western Blot
  12. Wnt4/b-catenin signaling induces VSMC proliferation and is associated with intimal thickening.
    Authors: Tsaousi A, Williams H, Lyon CA et al.
    Circ Res.

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