Mouse Wnt-8b Antibody Summary
-9a, recombinant mouse Wnt-1, -3a, -4, -5a, -5b, -8a, or -9b is observed.
Trp22-Glu52, Leu195-Arg269
Accession # Q9WUD6
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
![Detection of Mouse Wnt‑8b antibody by Western Blot. Detection of Mouse Wnt‑8b antibody by Western Blot.](https://resources.rndsystems.com/images/datasheets/antibody/Wnt-8b_MAB33671_Western_Blot_6095.jpg)
Detection of Mouse Wnt‑8b by Western Blot. Western blot shows lysates of KATO-III human gastric carcinoma cell line. PVDF membrane was probed with 5 µg/mL Rat Anti-Mouse Wnt-8b Monoclonal Antibody (Catalog # MAB33671) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). For additional reference, recombinant mouse Wnt-8b (20 ng) was included. A specific band for native Wnt-8b was detected at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 6.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Wnt-8b
Mouse Wnt-8b is a 40-50 kDa secreted glycoprotein member of the Wnt family of proteins. Mature mouse Wnt-8b is 329 aa in length and contains 24 cysteines plus three potential N-linked glycosylation sites. Little is known about Wnt-8b. It appears to play a role in forebrain development. The mature mouse protein is 100%, 98%, and 97% identical to mature rat, canine, and human Wnt-8b, respectively.
Product Datasheets
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