Home / YM1/Chitinase 3-like 3 / Mouse YM1/Chitinase 3-like 3 Antibody

Mouse YM1/Chitinase 3-like 3 Antibody

Catalog #: AF2446
16 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2446
AF2446-SP
Detection of Mouse YM1/Chitinase 3-like 3 by Western Blot.
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Product Details
Citations (16)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse YM1/Chitinase 3-like 3 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse YM1/Chitinase 3-like 3 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse YM1/Chitinase 3-like 3
Tyr22-Tyr398
Accession # O35744
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below
Simple Western
2.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse YM1/Chitinase 3-like 3 antibody by Western Blot. View Larger

Detection of Mouse YM1/Chitinase 3-like 3 by Western Blot. Western blot shows lysates of mouse bone marrow. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse YM1/Chitinase 3-like 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2446) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for YM1/Chitinase 3-like 3 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Mouse YM1/Chitinase 3-like 3 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Mouse YM1/Chitinase 3‑like 3 by Simple WesternTM. Simple Western lane view shows lysates of mouse bone marrow, loaded at 0.2 mg/mL. A specific band was detected for YM1/Chitinase 3-like 3 at approximately 49 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Mouse YM1/Chitinase 3-like 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2446) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: YM1/Chitinase 3-like 3

YM1, also known as Chitinase 3-like 3 and ECF-L, is a secreted 377 amino acid residue lectin that binds heparin and G1cN oligomers. It is produced primarily by macrophages during inflammation. YM1 shares homology with chitinase family proteins but lacks enzymatic activity.

Entrez Gene IDs
12655 (Mouse)
Alternate Names
AI505981; CHI3L3; Chitinase 3 like 3; Chitinase 3-like 3; ECF-L; YM1

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Citations for Mouse YM1/Chitinase 3-like 3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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  1. Sevoflurane activates the IL-6/HO-1 pathway to promote macrophage M2 polarization and prostate cancer lung metastasis
    Authors: F Yu, T Bai
    International immunopharmacology, 2022-10-30;113(0):109380.
    Species: Human
    Sample Types: Tissue Homogenate
    Applications: Western Blot
  2. Phagocytosis and macrophage polarization on bacterially contaminated dental implant materials and effects on tissue integration
    Authors: Y Yuan, Y Ren, M Dijk, GI Geertsema-, J Atema-Smit, HJ Busscher, HC van der Me
    European cells & materials, 2021-03-30;41(0):421-430.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  3. Midkine rewires the melanoma microenvironment toward a tolerogenic and immune-resistant state
    Authors: D Cerezo-Wal, M Contreras-, K Troulé, X Catena, C Mucientes, TG Calvo, E Cañón, C Tejedo, PC Pennacchi, S Hogan, P Kölblinger, H Tejero, AX Chen, N Ibarz, O Graña-Cast, L Martinez, J Muñoz, P Ortiz-Rome, JL Rodriguez-, G Gómez-Lópe, F Al-Shahrou, R Rabadán, MP Levesque, D Olmeda, MS Soengas
    Nat Med, 2020-10-19;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Inflammasome-Independent Role for NLRP3 in Controlling Innate Antihelminth Immunity and Tissue Repair in the Lung
    Authors: AL Chenery, R Alhallaf, Z Agha, J Ajendra, JE Parkinson, MM Cooper, BHK Chan, RM Eichenberg, LA Dent, AAB Robertson, A Kupz, D Brough, A Loukas, TE Sutherland, JE Allen, PR Giacomin
    J. Immunol., 2019-10-04;203(10):2724-2734.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Macrophages promote epithelial proliferation following infectious and non-infectious lung injury through a Trefoil factor 2-dependent mechanism
    Authors: LY Hung, D Sen, TK Oniskey, J Katzen, NA Cohen, AE Vaughan, W Nieves, A Urisman, MF Beers, MF Krummel, DR Herbert
    Mucosal Immunol, 2018-10-18;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. EMAPII Monoclonal Antibody Ameliorates Influenza A Virus-Induced Lung Injury
    Authors: H Lu, S Chelvanamb, C Poirier, J Saliba, KL March, M Clauss, NV Bogatcheva
    Mol. Ther., 2018-06-15;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  7. Dual role of YM1+ M2 macrophages in allergic lung inflammation
    Authors: C Draijer, P Robbe, CE Boorsma, MN Hylkema, BN Melgert
    Sci Rep, 2018-03-23;8(1):5105.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  8. Macrophage polarization and activation at the interface of multi-walled carbon nanotube-induced pulmonary inflammation and fibrosis
    Authors: J Dong, Q Ma
    Nanotoxicology, 2018-01-16;0(0):1-16.
    Species: Mouse
    Sample Types: Tissue Lysate, Whole Tissue
    Applications: IHC, Western Blot
  9. TNF?-stimulated gene-6 (TSG6) activates macrophage phenotype transition to prevent inflammatory lung injury
    Proc. Natl. Acad. Sci. U.S.A., 2016-11-28;113(50):E8151-E8158.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Diabetic Wounds Exhibit Decreased Ym1 and Arginase Expression with Increased Expression of IL-17 and IL-20
    Authors: Laurie P Shornick
    Adv Wound Care (New Rochelle), 2016-11-01;5(11):486-494.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. Severe Reduction in Number and Function of Peripheral T Cells Does Not Afford Protection toward Emphysema and Bronchial Remodeling Induced in Mice by Cigarette Smoke
    Am J Pathol, 2016-05-06;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  12. Smoking p66Shc knocked out mice develop respiratory bronchiolitis with fibrosis but not emphysema.
    Authors: Lunghi B, De Cunto G, Cavarra E, Fineschi S, Bartalesi B, Lungarella G, Lucattelli M
    PLoS ONE, 2015-03-19;10(3):e0119797.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  13. Biomarkers of disease and treatment in murine and cynomolgus models of chronic asthma.
    Authors: Louten J, Mattson JD, Malinao MC, Li Y, Emson C, Vega F, Wardle RL, Van Scott MR, Fick RB, McClanahan TK, de Waal Malefyt R, Beaumont M
    Biomark Insights, 2012-07-10;7(0):87-104.
    Species: Mouse
    Sample Types: BALF
    Applications: Western Blot
  14. Lack of IL-4 receptor expression on T helper cells reduces T helper 2 cell polyfunctionality and confers resistance in allergic bronchopulmonary mycosis.
    Authors: Muller U, Piehler D, Stenzel W, Kohler G, Frey O, Held J, Grahnert A, Richter T, Eschke M, Kamradt T, Brombacher F, Alber G
    Mucosal Immunol, 2012-02-15;5(3):299-310.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  15. Extrathymically generated regulatory T cells control mucosal TH2 inflammation.
    Authors: Josefowicz SZ, Niec RE, Kim HY
    Nature, 2012-02-08;482(7385):395-9.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  16. Hookworm-induced persistent changes to the immunological environment of the lung.
    Authors: Reece JJ, Siracusa MC, Southard TL, Brayton CF, Urban JF, Scott AL
    Infect. Immun., 2008-05-27;76(8):3511-24.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P

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