N21-MAX Vitamin A Free Media Supplement (50X)

For controlled neural stem and progenitor cell expansion.

Why Culture Neurons in Serum-Free N21-MAX Media Supplement?

The N21-MAX Vitamin A Media Supplement is a serum-free and fully-defined media formulation that is optimized for the consistent health and superior growth of neural stem/progenitor cells. The N21-MAX Vitamin A Free Supplement limits NSC differentiation by reducing unwanted retinoic acid production in cell culture medium. It can also be used to mature and maintain healthy primary neurons in culture.

N21-MAX media supplements were designed to eliminate uncontrolled variables, such as those found in both serum-containing media and in some commercial serum-free neuronal supplements. Use of undefined media supplements can confound neuronal growth and maturation. N21-MAX media supplements contain a cocktail of factors optimized for neural stem cell expansion, neuronal survival, and synaptic maturation. Each lot of N21-MAX Media Supplement is tested on primary hippocampal neurons to ensure consistent performance for the user.

The N21-MAX Vitamin A Free Media Supplement:

• Is fully defined and quality tested to reduce unwanted experimental variation.
• Contains factors qualified to the expansion of NSCs in vitro.
• Limits retinoic acid production to reduce unwanted NSC differentiation.
• Does not require an astrocyte feeder layer.

N21-MAX Vitamin A Free Media Supplement Components

N21-MAX Vitamin A-Free Media Supplement is optimized for the expansion of neural stem cells in culture. The supplement is supplied as a 50X concentrated solution and contains the following 19 components:

• Albumin (bovine)
• L-Carnitine
• Catalase
• Corticosterone
• Ethanolamine
• Glutathione
• Galactose
• Holo-Transferrin
• Insulin
• Linoleic Acid
• Linolenic Acid
• Lipoic Acid
• Progesterone
• Putrescine
• Selenite
• Superoxide dismutase
• Triiodo-L-thyronine
• D,L-alpha-Tocopherol
• D,L-alpha-Tocopherol acetate

Supplied in a volume sufficient to supplement 500 mL of media at the recommended concentration. N21-MAX Vitamin A Free Media Supplement is tested for use in Neurobasal Media.

Precautions

This product contains human transferrin. This transferrin was tested at the donor level using an FDA licensed method and found to be non-reactive for anti-HIV-1/2 and Hepatitis B surface antigen. As no testing can offer complete assurance of freedom from infectious agents, these reagents should be handled as if capable of transmitting infection.