Neuron-specific beta -III Tubulin Alexa Fluor® 405-conjugated Antibody

Catalog #: IC1195V Datasheet / COA / SDS

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IC1195V-100UG

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Neuron-specific beta -III Tubulin Alexa Fluor® 405-conjugated Antibody Summary

Specificity

Detects mammalian and chicken neuron-specific beta -III tubulin but not other beta -tubulin isotypes in Western blots.

Source

Monoclonal Mouse IgG_2A Clone # TuJ-1

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Rat brain-derived microtubules

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Label

Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

Titration recommended for optimal concentration with starting range of0.1-1 µg/1 million cells. Sample used for this experiment was HepG2 humanhepatocellular carcinoma cells.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: beta-III Tubulin

beta -III Tubulin, also known as tubulin beta -4, is regarded as a neuron-specific marker. The expression of beta -III Tubulin has been suggested to be one of the earliest markers to signal commitment in primitive neuroepithelium.

Entrez Gene IDs

10381 (Human)

Alternate Names

Beta3-tubulin; beta-4; betaIII Tubulin; beta-III Tubulin; beta-Tubulin III; Class III beta-tubulin; TUBB3; TUBB4 CFEOM3A; Tubulin Beta 3; tubulin beta-3 chain; Tubulin beta-4 chain; Tubulin beta-4; Tubulin beta-III; tubulin, beta 3; TUJ1 antigen; TUJ-1 antigen