NeuroTACS In Situ Apoptosis Detection Kit
NeuroTACS In Situ Apoptosis Detection Kit Summary
Provides rapid and convenient identification of apoptosis in brain tissue or neuronal cells using a TUNEL based assay.Key Benefits
• Unique buffer system produces more consistent labeling.
• Performance tested on brain sections.
• Includes exclusive NeuroPore permeabilization reagent.
• Includes TACS-Nuclease solution for preparing sample-dependent positive controls.
• Helps resolve unique problems encountered when detecting apoptotic neuronal cells.
Why Use the NeuroTACS In Situ Apoptosis Detection Kit?
The kit has been developed to overcome the common difficulties unique to neuronal samples including the fragile nature of brain tissue sections, high background problems, poor counterstaining with common dyes, and the need to perform dual labeling experiments to detect cell specific antigens in conjunction with apoptotic cells. A key feature is NeuroPore, a proprietary permeabilization reagent that gently permeabilizes samples while retaining cell morphology. NeuroPore also contains blocking reagents to allow its use as an antibody diluent in immunohistochemistry and to reduce background staining.
Kit Contents
• Proteinase K
• Streptavidin-HRP
• Diaminobenzidine
• DAB Enhancer
• TACS-Nuclease
• TACS-Nuclease Buffer
• TACS 2 TdT Labeling Buffer
• TACS 2 TdT Stop Buffer
• TACS 2 TdT dNTP
• TdT Enzyme
• 50x Manganese Cation
• NeuroPore
• Blue Counterstain
Specifications
Limitations
For research use only. Not for diagnostic use.
Product Datasheets
Citations for NeuroTACS In Situ Apoptosis Detection Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Mycoplasma DnaK increases DNA copy number variants in vivo
Authors: Benedetti, F;Silvestri, G;Saadat, S;Denaro, F;Latinovic, OS;Davis, H;Williams, S;Bryant, J;Ippodrino, R;Rathinam, CV;Gallo, RC;Zella, D;
Proceedings of the National Academy of Sciences of the United States of America 2023-07-25
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Vertical Sleeve Gastrectomy Offers Protection against Disturbed Flow-Induced Atherosclerosis in High-Fat Diet-Fed Mice
Authors: JH Wei, WJ Lee, JL Luo, HL Huang, SC Wang, RH Chou, PH Huang, SJ Lin
International Journal of Molecular Sciences, 2023-03-16;24(6):. 2023-03-16
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Mechanical force regulates root resorption in rats through RANKL and OPG
Authors: J Zhou, L Guo, Y Yang, Y Liu, C Zhang
BMC Oral Health, 2022-07-16;22(1):290. 2022-07-16
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Targeted Mutations in the Fusion Peptide Region of La Crosse Virus Attenuate Neuroinvasion and Confer Protection against Encephalitis
Authors: BS Hollidge, MV Salzano, JM Ibrahim, JW Fraser, V Wagner, NE Leitner, SR Weiss, F Weber, F González-S, SS Soldan
Viruses, 2022-07-02;14(7):. 2022-07-02
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Methodology and Neuromarkers for Cetaceans' Brains
Authors: S Sacchini, P Herráez, M Arbelo, A Espinosa d, E Sierra, M Rivero, C Bombardi, A Fernández
Veterinary sciences, 2022-01-21;9(2):. 2022-01-21
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Zebrafish Blunt-Force TBI Induces Heterogenous Injury Pathologies That Mimic Human TBI and Responds with Sonic Hedgehog-Dependent Cell Proliferation across the Neuroaxis
Authors: J Hentig, K Cloghessy, M Lahne, YJ Jung, RA Petersen, AC Morris, DR Hyde
Biomedicines, 2021-07-22;9(8):. 2021-07-22
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Intra-cisterna magna delivery of an AAV vector with the GLUT1 promoter in a pig recapitulates the physiological expression of SLC2A1
Authors: S Nakamura, H Osaka, SI Muramatsu, N Takino, M Ito, EF Jimbo, C Watanabe, S Hishikawa, T Nakajima, T Yamagata
Gene Ther, 2020-10-19;0(0):. 2020-10-19
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Synaptic synthesis, dephosphorylation, and degradation: a novel paradigm for an activity-dependent neuronal control of CDKL5.
Authors: La Montanara P, Rusconi L, Locarno A, Forti L, Barbiero I, Tramarin M, Chandola C, Kilstrup-Nielsen C, Landsberger N
J Biol Chem, 2015-01-02;290(7):4512-27. 2015-01-02
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Hypoxia inducible factor-1alpha (HIF-1alpha) is required for neural stem cell maintenance and vascular stability in the adult mouse SVZ.
Authors: Li L, Candelario K, Thomas K, Wang R, Wright K, Messier A, Cunningham L
J Neurosci, 2014-12-10;34(50):16713-9. 2014-12-10
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Differences in the phagocytic response of microglia and peripheral macrophages after spinal cord injury and its effects on cell death.
Authors: Greenhalgh A, David S
J Neurosci, 2014-04-30;34(18):6316-22. 2014-04-30
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Microcirculatory, mitochondrial, and histological changes following cerebral ischemia in swine.
Authors: Suchadolskiene, Olga, Pranskunas, Andrius, Baliutyte, Giedre, Veikutis, Vincenta, Dambrauskas, Zilvinas, Vaitkaitis, Dinas, Borutaite, Vilmante
BMC Neurosci, 2014-01-03;15(0):2. 2014-01-03
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Impaired autophagy in neurons after disinhibition of mammalian target of rapamycin and its contribution to epileptogenesis.
Authors: McMahon J, Huang X, Yang J, Komatsu M, Yue Z, Qian J, Zhu X, Huang Y
J Neurosci, 2012-11-07;32(45):15704-14. 2012-11-07
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The splicing regulator Rbfox2 is required for both cerebellar development and mature motor function.
Authors: Gehman L, Meera P, Stoilov P, Shiue L, O'Brien J, Meisler M, Ares M, Otis T, Black D
Genes Dev, 2012-02-22;26(5):445-60. 2012-02-22
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Chronic application of nonylphenol-induced apoptosis via suppression of bcl-2 transcription and up-regulation of active caspase-3 in mouse brain.
Authors: Mao Z, Zheng YL, Zhang YQ, Han BP, Chen LT, Li J, Li F, Shan Q
Neurosci. Lett., 2008-05-08;439(2):147-52. 2008-05-08
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