Ocean Blue, SE

Biological Activity

Application: Flow cytometry, fluorescence microscopy.

Properties and Photophysical Data: Conjugates of this dye are strongly fluorescent even at neutral pH. Excitation and emission maxima (λ) are 405 nM and 455 nM, respectively; quantum yield = 0.78; extinction coefficient = 36,000 M^-1cm^-1; A280 correction factor = 0.15.

Please see the protocol for further information on protein/antibody labeling and conjugation.

Note: This product is typically prepared in DMSO.

Optical Data for Ocean Blue, SE

Technical Data

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Background References

1. Synthesis of novel fluorinated coumarins: excellent UV-light excitable fluorescent dyes.
   Sun et al.
   Bioorg.Med.Chem.Lett., 1998;8:3107
2. The increasing power of immunohistochemistry and immunocytochemistry.
   Brandtzaeg et al.
   J.Immunol.Methods, 1998;216:49
3. Antibody conjugates for cell biology.
   Haugland et al.
   Curr.Protoc.Cell Biol., 2001;
4. Fluorescent DNA hybridization probe preparation using amine modification and reactive dye coupling.
   Cox et al.
   Biotechniques, 2004;36:114

Product Datasheets