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Oxidized PTP Active Site Antibody

Catalog #: MAB2844
11 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB2844
MAB2844-SP
Detection of Oxidized PTP by Western Blot.
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Product Details
Citations (11)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Oxidized PTP Active Site Antibody Summary

Specificity
Detects Oxidized PTP Active Site. Will react with any oxidized phosphatase containing the consensus sequence. Detects oxidized but not unoxidized DEP-1, PTP1B, TC-PTP, and SHP-2. Other phosphatases have not been tested.
Source
Monoclonal Mouse IgG1 Clone # 335636
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
KLH-coupled perfomic acid oxidized synthetic peptide
VHCSAG
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Oxidized PTP antibody by Western Blot. View Larger

Detection of Oxidized PTP by Western Blot. Western blot shows oxidized PTP immunoprecipitated from lysates of HepG2 human hepatocellular carcinoma cell line using Rat Anti-SHP-2 antibody. PVDF membrane was probed with 1 µg/mL Mouse Anti-Oxidized PTP Active Site Monoclonal Antibody (Catalog # MAB2844) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for Oxidized SHP-2 was detected at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Oxidized PTP Active Site

Oxidation of the essential cysteine in the active site of protein tyrosine phosphatases (PTPs) by exogenous or intracellular oxidants such as hydrogen peroxide is believed to play an important role in regulating the activity of these enzymes. The initial oxidation to a sulfenic acid (-SOH) is reversible, but the oxidation often progresses to an irreversible sulfonic acid (-SO3H) that can be detected by modification-specific antibodies. The active sites of PTPs contain the consensus amino acid sequence (V/I)HCSXG, a sequence essential for catalytic activity.

Long Name
Oxidized Protein Tyrosine Phosphatase Active Site
Alternate Names
Oxidized PTP Active Site

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Citations for Oxidized PTP Active Site Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. Redox cycling of 9,10-phenanthrenequinone activates epidermal growth factor receptor signaling through S-oxidation of protein tyrosine phosphatase 1B
    Authors: NC Luong, Y Abiko, T Shibata, K Uchida, E Warabi, M Suzuki, T Noguchi, A Matsuzawa, Y Kumagai
    J Toxicol Sci, 2020-01-01;45(6):349-363.
    Species: Human
    Sample Types: Protein, Whole Cells
    Applications: Neutralization, Western Blot
  2. Benzoquinone, a leukemogenic metabolite of benzene, catalytically inhibits the protein tyrosine phosphatase PTPN2 and alters STAT1 signaling
    Authors: R Duval, LC Bui, C Mathieu, Q Nian, J Berthelet, X Xu, I Haddad, J Vinh, JM Dupret, F Busi, F Guidez, C Chomienne, F Rodrigues-
    J. Biol. Chem., 2019-06-27;0(0):.
    Applications: Western Blot
  3. Upregulation of Nrf2 and Decreased Redox Signaling Contribute to Renoprotective Effects of Chemerin Receptor Blockade in Diabetic Mice
    Authors: KB Neves, AC Montezano, R Alves-Lope, T Bruder-Nas, RM Costa, RS Costa, RM Touyz, RC Tostes
    Int J Mol Sci, 2018-08-19;19(8):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  4. Peroxiredoxin 2 mediates insulin sensitivity of skeletal muscles through regulation of protein tyrosine phosphatase oxidation
    Authors: JH Kim, SJ Park, U Chae, J Seong, HS Lee, SR Lee, S Lee, DS Lee
    Int. J. Biochem. Cell Biol., 2018-03-29;99(0):80-90.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. The pseudophosphatase phogrin enables glucose-stimulated insulin signaling in pancreatic ?-cells
    Authors: S Torii, C Kubota, N Saito, A Kawano, N Hou, M Kobayashi, R Torii, M Hosaka, T Kitamura, T Takeuchi, H Gomi
    J. Biol. Chem., 2018-02-26;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Differential oxidation of protein-tyrosine phosphatases during zebrafish caudal fin regeneration
    Authors: W Wu, AJ Hale, S Lemeer, J den Hertog
    Sci Rep, 2017-08-16;7(1):8460.
    Species: Zebrafish
    Sample Types: Tissue Homogenates
    Applications: Immunoprecipitation
  7. THEMIS enhances TCR signaling and enables positive selection by selective inhibition of the phosphatase SHP-1
    Authors: S Choi, C Warzecha, E Zvezdova, J Lee, J Argenty, R Lesourne, L Aravind, PE Love
    Nat. Immunol, 2017-02-27;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Dual oxidase 1 induced by Th2 cytokines promotes STAT6 phosphorylation via oxidative inactivation of protein tyrosine phosphatase 1B in human epidermal keratinocytes.
    Authors: Hirakawa S, Saito R, Ohara H
    J. Immunol., 2011-03-16;186(8):4762-70.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Redox regulation of interleukin-4 signaling.
    Authors: Sharma P, Chakraborty R, Wang L, Min B, Tremblay ML, Kawahara T, Lambeth JD, Haque SJ
    Immunity, 2008-10-17;29(4):551-64.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  10. Cysteine S-nitrosylation protects protein-tyrosine phosphatase 1B against oxidation-induced permanent inactivation.
    Authors: Chen YY, Chu HM, Pan KT, Teng CH, Wang DL, Wang AH, Khoo KH, Meng TC
    J. Biol. Chem., 2008-10-07;283(50):35265-72.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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