Phospho-Dynamin (S774) Antibody

Catalog #: PPS004 Datasheet / COA / SDS

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PPS004

Detection of Phospho-Dynamin (S774) by Western Blot.

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Phospho-Dynamin (S774) Antibody Summary

Species Reactivity

Human, Mouse, Rat, Bovine, Canine, Chicken, Primate

Specificity

Human, mouse, rat, bovine, canine, chicken, and primate ~95 kDa Dynamin protein phosphorylated at S774. Labels the purified protein phosphorylated in vitro by cdk5 but not by PKC. Does not cross react with other purified substrates of cdk5 (e.g., Amphiphysin and Synapsin).

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Phosphopeptide corresponding to amino acid residues surrounding phospho-S774 of Dynamin

Formulation

100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1:1000 dilution

Rat brain (hippocampus) tissue

Immunohistochemistry

1:1000 dilution

Fixed culture neurons

Immunofluorescence

1:1000 dilution

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunofluorescence

Detection of Phospho-Dynamin (S774) antibody by Western Blot.

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Detection of Phospho-Dynamin (S774) by Western Blot. Western blot of rat brain (hippocampus) tissue lysate stimulated with forskolin showing specific immunolabeling of the ~95 kDa dynamin phosphorylated at S774 (Control). The phosphospecificity of this labeling is dem-onstrated by treatment with 1200 U of lambda Phosphatase ( lambda -PPase) for 30 minutes before being exposed to the Anti-Phospho-Dynamin (S774). The immunolabeling is completely eliminated by treatment with lambda -PPase.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

For long-term storage, = -20° C is recommended. Product is stable at = -20° C for at least 1 year.

Background: Dynamin

Rat Dynamin (or Dynamin 1; also known as dephosphin) is a 95-100 kDa GTPase that belongs to the “classic dynamin” group of the dynamin superfamily. It is 864 amino acids (aa) in length, and contains three N-terminal GTP-binding elements (aa 38-208), one pleckstrin-homology domain (aa 519-625), a GED (GTPase effector domain) (aa 645-745), and a C-terminal SH3-binding proline-rich region (aa 753-864). At least four alternate splice forms are known, one that shows a 47 aa substitution between aa 398-445, and three that generate unique C-termini over the last 20 amino acids. Dynamin is found constitutively phosphorylated and inactive in the cytoplasm just under synaptic membranes. Upon stimulation, calcineurin dephosphorylates, and nitric oxide nitrosylates, Dynamin. Dephosphorylated and nitrosylated Dynamin now interacts with the cell membrane, generating endocytic synaptic vesicles. The process terminates when Cdk5 rephosphorylates Dynamin on S744 and S778.

Entrez Gene IDs

1759 (Human)

Alternate Names

DNM; DNM1; dynamin 1; Dynamin; dynamin-1; EC 3.6.5.5