Home / GluR1 / Phospho-GluR1 (S831) Antibody

Phospho-GluR1 (S831) Antibody

Catalog #: PPS007
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
PPS007
Detection of Phospho-GluR1 (S831) by Western Blot.
1 Image
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Phospho-GluR1 (S831) Antibody Summary

Species Reactivity
Human, Mouse, Rat, Canine, Chicken
Specificity
Specific for the ~100 kDa GluR1 protein phosphorylated at S831 in Western blots
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
Phosphopeptide corresponding to amino acid residues surrounding the phospho-S831 of GluR1
Formulation
150 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1:1000 dilution
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Phospho-GluR1 (S831) antibody by Western Blot. View Larger

Detection of Phospho-GluR1 (S831) by Western Blot. Western blot of rat hippocampal lysate showing specific immunolabeling of the ~100 kDa GluR1 protein phosphorylated at S831 (Control). The phosphospecificity of this labeling is demonstrated by treatment with 1200 U of lambda Phosphatase ( lambda -PPase) for 30 minutes before being exposed to the Anti-Phospho-GluR1 (S831). The immunolabeling is completely eliminated by treatment with lambda -PPase.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: GluR1

Rat GluR1 is a 907 amino acid, 4-transmembrane protein that belongs to the glutamate-gated ion channel family. It is one of four AMPA receptor subunits that form a functional heterotetrameric glutamate receptor. GluR1 only interacts with GluR2. GluR1 has two key serine residues in the C-terminal extracellular region. Serine 831 is constitutively unphosphorylated. Upon exposure to a neurotransmitter, it is phosphorylated by CaMKII leading to a potentiation of glutamate-mediated current. Serine 845 is constitutively phosphorylated by Protein Kinase A (PKA). The presence of the phosphate insures membrane localization of the subunit. Following NMDA receptor activation, S845 is dephosphorylated with subsequent subunit internalization.

Long Name
Glutamate Receptor 1
Entrez Gene IDs
2890 (Human); 14799 (Mouse); 50592 (Rat)
Alternate Names
AMPA 1; AMPA-selective glutamate receptor 1; GluA1; GLUH1; GluR1; gluR-1; GLUR1gluR-A; GLURA; GluR-A; gluR-K1; glutamate receptor 1; Glutamate receptor ionotropic, AMPA 1; glutamate receptor, ionotropic, AMPA 1; GRIA1; HBGR1; MGC133252

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citation for Phospho-GluR1 (S831) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Endosomal Dysfunction Induced by Directly Overactivating Rab5 Recapitulates Prodromal and Neurodegenerative Features of Alzheimer's Disease
    Authors: A Pensalfini, S Kim, S Subbanna, C Bleiwas, CN Goulbourne, PH Stavrides, Y Jiang, JH Lee, S Darji, M Pawlik, C Huo, J Peddy, MJ Berg, JF Smiley, BS Basavaraja, RA Nixon
    Cell Rep, 2020-11-24;33(8):108420.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Phospho-GluR1 (S831) Antibody

There are currently no reviews for this product. Be the first to review Phospho-GluR1 (S831) Antibody and earn rewards!

Have you used Phospho-GluR1 (S831) Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review