Phospho-Synapsin I (S9) Antibody

Catalog #: PPS084 Datasheet / COA / SDS

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PPS084

Detection of Phospho-Synapsin I (S9) by Western Blot.

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Phospho-Synapsin I (S9) Antibody Summary

Species Reactivity

Human, Mouse, Rat, Xenopus

Specificity

Human, rat, mouse, and Xenopus ~78 kDa Synapsin I doublet protein phosphorylated at S9

Source

Polyclonal Rabbit IgG

Purification

Antigen Affinity-purified

Immunogen

Phosphopeptide corresponding to amino acid residues surrounding the phospho-S9 of Synapsin I

Formulation

100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1:1000 dilution

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Phospho-Synapsin I (S9) by Western Blot. Western blot of rat brain lysate showing specific immunolabeling of the approximately 78 kDa Synapsin I phosphorylated at S9 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda PPase). The blot is identical to the control except that it was incubated in lambda PPase (1200 units for 30 minutes) before being exposed to the anti-phospho-S9 Synapsin I. The immunolabeling is completely eliminated by treatment with lambda PPase.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: Synapsin I

Synapsin I is an 85 kDa neuron-specific, O-GlcNAcylated phosphoprotein that interacts with synaptic vesicles and regulates neurotransmitter release. It is concentrated in presynaptic terminals where it anchors synaptic vesicles to the underlying cytoskeleton. Phosphorylation at various sites reduces synapsin I binding to neurosecretory vesicles, making them available for neurotransmitter release. Rat synapsin I is 704 amino acids (aa) in length and contains five domains, named A - E/F. Domains A - D lie between aa 1 - 655, while E and F are alternate splice forms. Domain A (28 aa) regulates neurotransmitter release in a phosphorylation-dependent manner. Domains C (328 aa) and E (49 aa) anchor to actin in the synaptic region. Domains B (84 aa) and D (235 aa) promote actin and secretory vesicle interaction, and actin nucleation. Phosphorylation of S603 and 566 in domain D, and S9 in domain A, reduces the affinity of synapsin I for actin and secretory vesicles.

References

Yamagata, Y. (2003) J. Pharmacol. Sci. 93:22.
Sakurada, K. et al. (2002) J. Biol. Chem. 277:45473.
Hilfiker, S. et al. (2005) J. Neurosci. 25:2658.
McCaffery, C.A. and L.J. DeGennaro (1986) EMBO J. 5:3167.
Cole, R.N. and G.W. Hart (1999) J. Neurochem. 73:418.

Entrez Gene IDs

6853 (Human); 20964 (Mouse); 24949 (Rat)

Alternate Names

SYN1; SYN1a; SYN1b; Synapsin 1; Synapsin I; synapsin IBrain protein 4.1; synapsin-1; SYNI

Product Datasheets

Product Datasheet

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