Porcine/Equine CD14 Alexa Fluor® 700-conjugated Antibody

Catalog #: FAB4597N Datasheet / COA / SDS

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FAB4597N-100UG

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Porcine/Equine CD14 Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity

Porcine, Equine

Specificity

Detects porcine CD14 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CD14 and recombinant mouse CD14 is observed.

Source

Monoclonal Mouse IgG2b Clone # 433423

Immunogen

E. coli-derived recombinant porcine CD14

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Label

Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25-1 µg/10⁶ cells

Porcine peripheral blood mononuclear cells (untreated) and equine peripheral blood mononuclear cells (PBMCs) treated with PMA and Calcium Ionomycin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CD14

CD14 is a 55 kDa GPI-linked cell surface glycoprotein that is preferentially expressed on monocytes/macrophages. It is a pattern recognition protein that acts as a receptor for lipopolysaccharide (LPS) and other microbial cell wall components. CD14 also occurs as a secreted protein. Mature porcine CD14 shares 70‑75% aa identity with human, cow, sheep, water buffalo and horse CD14 and 61‑62% aa identity with mouse and rat CD14.

Entrez Gene IDs

929 (Human); 12475 (Mouse); 60350 (Rat); 100037938 (Porcine)

Alternate Names

CD14 antigen; CD14 molecule; CD14; monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein

Product Datasheets

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Product Specific Notices

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.