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Porcine IL-1 beta/IL-1F2 ELISA Kit - Quantikine

Catalog #: PLB00B
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Porcine IL-1 beta/IL-1F2 ELISA Standard Curve
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Citations (41)
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Porcine IL-1 beta/IL-1F2 Quantikine ELISA Kit Summary

Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (25 uL), Serum (50 uL), Heparin Plasma (50 uL)
Sensitivity
13.6 pg/mL
Assay Range
39.1 - 2,500 pg/mL (Cell Culture Supernates, Serum, Heparin Plasma)
Specificity
Natural and recombinant porcine IL-1 beta
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Porcine IL-1 beta Immunoassay is a 4.5 hour solid phase ELISA designed to measure porcine IL-1 beta in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mature porcine IL-1 beta and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant porcine IL-1 beta. Results obtained using natural porcine IL-1 beta show dose response curves that are parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural porcine IL-1 beta.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 136 348 780 141 338 783
Standard Deviation 9.8 17 40.9 12.2 20.5 31.7
CV% 7.2 4.9 5.2 8.7 6.1 4

Recovery

The recovery of porcine IL-1 beta spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 106 102-109
Heparin Plasma (n=4) 111 98-117
Serum (n=4) 111 102-117

Linearity

To assess the linearity of the assay, samples containing and/or spiked with various concentrations of porcine IL-1 beta in each matrix were diluted with Calibrator Diluent and then assayed.
Porcine IL-1 beta/IL-1F2 ELISA Linearity

Scientific Data

N/A Porcine IL-1 beta/IL-1F2 ELISA Standard Curve View Larger

Porcine IL-1 beta/IL-1F2 ELISA Standard Curve

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-1 beta/IL-1F2

IL-1 beta (Interleukin-1 beta) is produced in response to inflammatory agents, infections, or microbial endotoxins. It plays a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. IL-1 beta binds to IL-1 RI and IL-1 RII. The IL-1 receptor accessory protein (IL-1 RAcP) associates with IL-1 RI and is required for IL-1 RI signal transduction, and IL-1ra is a secreted molecule that functions as a competitive inhibitor of IL-1 bioactivity.

Long Name:
Interleukin 1 beta
Entrez Gene IDs:
3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
Alternate Names:
catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Porcine IL-1 beta/IL-1F2 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

41 Citations: Showing 1 - 10
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  1. PRRSV infection activates NLRP3 inflammasome through inducing cytosolic mitochondrial DNA stress
    Authors: H Li, X Yang, Y Song, Q Zhu, Z Liao, Y Liang, J Guo, B Wan, D Bao
    Veterinary Microbiology, 2023-02-03;279(0):109673.
    Species: Porcine
    Sample Types: Cell Culture Supernates
  2. Supplementation With Spirulina Reduces Infarct Size and Ameliorates Cardiac Function in a Pig Model of STEMI
    Authors: G Vilahur, P Sutelman, S Ben-Aicha, G Mendieta, M Radiké, L Schoch, L Casaní, M Borrell-Pa, T Padro, L Badimon
    Frontiers in Pharmacology, 2022-05-03;13(0):891801.
    Species: Porcine
    Sample Types: Plasma
  3. Hemosiderin Accumulation in Liver Decreases Iron Availability in Tachycardia-Induced Porcine Congestive Heart Failure Model
    Authors: M Kasztura, L Kiczak, U Pas?awska, J Bania, A Janiszewsk, A Tomaszek, M Zacharski, A Noszczyk-N, R Pas?awski, A Tabi?, P Kuropka, P Dzi?giel, P Ponikowski
    International Journal of Molecular Sciences, 2022-01-18;23(3):.
    Species: Porcine
    Sample Types: Tissue Homogenates
  4. Identification of Virulence Associated Region during Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus during Attenuation In Vitro: Complex Question with Different Strain Backgrounds
    Authors: Y Jiang, W Tong, L Yu, L Li, F Gao, G Li, C Liu, P Chen, Q Shen, Y Zhang, Y Zhou, G Tong
    Viruses, 2021-12-27;14(1):.
    Species: Porcine
    Sample Types: Serum
  5. A novel pre-clinical strategy to deliver antimicrobial doses of inhaled nitric oxide
    Authors: VS Michaelsen, RVP Ribeiro, E Brambate, A Ali, A Wang, L Pires, M Kawashima, Y Zhang, A Gazzalle, S Keshavjee, L Del Sorbo, M Cypel
    PLoS ONE, 2021-10-13;16(10):e0258368.
    Species: Porcine
    Sample Types: Tissue Homogenates
  6. Epigenetic Modulation of TLR4 Expression by Sulforaphane Increases Anti-Inflammatory Capacity in Porcine Monocyte-Derived Dendritic Cells
    Authors: X Qu, C Neuhoff, MU Cinar, M Pröll, E Tholen, D Tesfaye, M Hölker, K Schellande, MJ Uddin
    Biology, 2021-05-31;10(6):.
    Species: Porcine
    Sample Types: Cell Culture Supernates
  7. Thymol modulates the endocannabinoid system and gut chemosensing of weaning pigs
    Authors: A Toschi, B Tugnoli, B Rossi, A Piva, E Grilli
    BMC Vet. Res., 2020-08-12;16(1):289.
    Species: Porcine
    Sample Types: Tissue Homogenates, Tissue Lysates
  8. Basic information for the development of a toxicity assay in inactivated bacterial vaccines
    Authors: EM Colella, GL Alborali, S Dotti, M Amadori
    Res. Vet. Sci., 2020-07-31;132(0):386-392.
    Species: Porcine
    Sample Types: Cell Culture Supernates
  9. Effects of sustained inflation pressure during�neonatal cardiopulmonary resuscitation of asphyxiated piglets
    Authors: GH Shim, SY Kim, PY Cheung, TF Lee, M O'Reilly, GM Schmölzer
    PLoS ONE, 2020-06-23;15(6):e0228693.
    Species: Porcine
    Sample Types: Tissue Homogenates
  10. Substitution of warthog NF-kappaB motifs into RELA of domestic pigs is not sufficient to confer resilience to African swine fever virus
    Authors: S McCleary, R Strong, RR McCarthy, JC Edwards, EL Howes, LM Stevens, PJ Sánchez-Co, A Núñez, S Watson, AJ Mileham, SG Lillico, C Tait-Burka, C Proudfoot, M Ballantyne, CBA Whitelaw, F Steinbach, HR Crooke
    Sci Rep, 2020-06-02;10(1):8951.
    Species: Porcine
    Sample Types: Serum
  11. Sepsis causes right ventricular myocardial inflammation independent of pulmonary hypertension in a porcine sepsis model
    Authors: SE Pischke, S Hestenes, HT Johansen, H Fure, JF Bugge, A Espinoza, H Skulstad, T Edvardsen, E Fosse, TE Mollnes, PS Halvorsen, EW Nielsen
    PLoS ONE, 2019-06-27;14(6):e0218624.
    Species: Porcine
    Sample Types: Plasma
  12. PO2 oscillations induce lung injury and inflammation
    Authors: S Boehme, EK Hartmann, T Tripp, SC Thal, M David, D Abraham, JE Baumgardne, K Markstalle, KU Klein
    Crit Care, 2019-03-27;23(1):102.
    Species: Porcine
    Sample Types: Plasma
  13. Medium-Chain Triglycerides Attenuate Liver Injury in Lipopolysaccharide-Challenged Pigs by Inhibiting Necroptotic and Inflammatory Signaling Pathways
    Authors: L Zhang, X Wang, S Chen, S Wang, Z Tu, G Zhang, H Zhu, X Li, J Xiong, Y Liu
    Int J Mol Sci, 2018-11-21;19(11):.
    Species: Porcine
    Sample Types: Serum
  14. Pulmonary Effects of Remote Ischemic Preconditioning in a Porcine Model of Ventilation-induced Lung Injury
    Authors: A Bergmann, T Schilling, G Hedenstier, K Ahlgren, A Larsson, M Kretzschma, A Kozian, T Hachenberg
    Respir Physiol Neurobiol, 2018-08-31;0(0):.
    Species: Porcine
    Sample Types: Serum
  15. Inflammatory markers before and after farrowing in healthy sows and in sows affected with postpartum dysgalactia syndrome
    Authors: M Kaiser, M Jacobson, PH Andersen, P Bækbo, JJ Cerón, J Dahl, D Escribano, S Jacobsen
    BMC Vet. Res., 2018-03-12;14(1):83.
    Species: Porcine
    Sample Types: Serum
  16. Influence of zinc supplementation on immune parameters in weaned pigs
    Authors: V Kloubert, K Blaabjerg, TS Dalgaard, HD Poulsen, L Rink, I Wessels
    J Trace Elem Med Biol, 2018-01-31;0(0):.
    Species: Porcine
    Sample Types: Whole Blood
    Applications: ELISA Capture
  17. An Immunomodulatory Device Improves Insulin Resistance in Obese Porcine Model of Metabolic Syndrome
    Authors: Angela J Westover
    J Diabetes Res, 2016-10-13;2016(0):3486727.
    Species: Porcine
    Sample Types: Plasma
  18. Sulforaphane epigenetically regulates innate immune responses of porcine monocyte-derived dendritic cells induced with lipopolysaccharide.
    Authors: Qu X, Proll M, Neuhoff C, Zhang R, Cinar M, Hossain M, Tesfaye D, Grosse-Brinkhaus C, Salilew-Wondim D, Tholen E, Looft C, Holker M, Schellander K, Uddin M
    PLoS ONE, 2015-03-20;10(3):e0121574.
    Species: Porcine
    Sample Types: Cell Culture Supernates
  19. Ex vivo adenoviral vector gene delivery results in decreased vector-associated inflammation pre- and post-lung transplantation in the pig.
    Authors: Yeung JC, Wagnetz D, Cypel M, Rubacha M, Koike T, Chun YM, Hu J, Waddell TK, Hwang DM, Liu M, Keshavjee S
    Mol. Ther., 2012-03-27;20(6):1204-11.
    Species: Porcine
    Sample Types: Plasma
  20. Ornithodoros moubata complement inhibitor is an equally effective C5 inhibitor in pigs and humans.
    Authors: Barratt-Due A, Thorgersen EB, Lindstad JK, Pharo A, Lissina O, Lambris JD, Nunn MA, Mollnes TE
    J. Immunol., 2011-09-30;187(9):4913-9.
    Species: Porcine
    Sample Types: Plasma
  21. Dissecting the effects of lipopolysaccharides from nonlipopolysaccharide molecules in experimental porcine meningococcal sepsis.
    Authors: Hellerud BC, Nielsen EW, Thorgersen EB, Lindstad JK, Pharo A, Tonnessen TI, Castellheim A, Mollnes TE, Brandtzaeg P
    Crit. Care Med., 2010-06-01;38(6):1467-74.
    Species: Porcine
    Sample Types: Plasma
  22. Plasma concentrations of inflammatory cytokines rise rapidly during ECMO-related SIRS due to the release of preformed stores in the intestine.
    Authors: McILwain RB, Timpa JG, Kurundkar AR
    Lab. Invest., 2009-11-09;90(1):128-39.
    Species: Porcine
    Sample Types: Plasma
  23. Cardiac Function and the Proinflammatory Cytokine Response After Recovery From Cardiac Arrest in Swine.
    Authors: Niemann JT, Rosborough JP, Youngquist S, Shah AP, Lewis RJ, Phan QT, Filler SG
    J. Interferon Cytokine Res., 2009-11-01;0(0):.
    Species: Porcine
    Sample Types: Plasma
  24. CD14 inhibition efficiently attenuates early inflammatory and hemostatic responses in Escherichia coli sepsis in pigs.
    Authors: Thorgersen EB, Hellerud BC, Nielsen EW, Barratt-Due A, Fure H, Lindstad JK, Pharo A, Fosse E, Tonnessen TI, Johansen HT, Castellheim A, Mollnes TE
    FASEB J., 2009-10-19;24(3):712-22.
    Species: Porcine
    Sample Types: Plasma
  25. Metabolic activity of the enteric microbiota influences the fatty acid composition of murine and porcine liver and adipose tissues.
    Authors: Wall R, Ross RP, Shanahan F, O'Mahony L, O'Mahony C, Coakley M, Hart O, Lawlor P, Quigley EM, Kiely B, Fitzgerald GF, Stanton C
    Am. J. Clin. Nutr., 2009-04-08;89(5):1393-401.
    Species: Porcine
    Sample Types: Cell Culture Supernates
  26. Inhibition of complement and CD14 attenuates the Escherichia coli-induced inflammatory response in porcine whole blood.
    Authors: Thorgersen EB, Pharo A, Haverson K, Axelsen AK, Gaustad P, Kotwal GJ, Sfyroera G, Mollnes TE
    Infect. Immun., 2008-12-01;77(2):725-32.
    Species: Porcine
    Sample Types: Whole Blood
  27. Blood concentrations of the cytokines IL-1beta, IL-6, IL-10, TNF-alpha and IFN-gamma during experimentally induced swine dysentery.
    Authors: Kruse R, Essen-Gustavsson B, Fossum C, Jensen-Waern M
    Acta Vet. Scand., 2008-08-12;50(0):32.
    Species: Porcine
    Sample Types: Serum
  28. Effects of glucocorticoids in ventilated piglets with severe pneumonia.
    Authors: Sibila O, Luna CM, Agusti C, Baquero S, Gando S, Patron JR, Morato JG, Absi R, Bassi N, Torres A
    Eur. Respir. J., 2008-05-28;32(4):1037-46.
    Species: Porcine
    Sample Types: Serum
  29. Cytokine secretion depends on Galalpha(1,3)Gal expression in a pig-to-human whole blood model.
    Authors: Saethre M, Schneider MK, Lambris JD, Magotti P, Haraldsen G, Seebach JD, Mollnes TE
    J. Immunol., 2008-05-01;180(9):6346-53.
    Species: Porcine
    Sample Types: Cell Culture Supernates
  30. Experimental Pseudomonas aeruginosa pneumonia: evaluation of the associated inflammatory response.
    Authors: Sibila O, Agusti C, Torres A, Baquero S, Gando S, Patron JR, Morato JG, Goffredo DH, Bassi N, Luna CM
    Eur. Respir. J., 2007-09-05;30(6):1167-72.
    Species: Porcine
    Sample Types: Serum
  31. The fibrin-derived peptide Bbeta15-42 is cardioprotective in a pig model of myocardial ischemia-reperfusion injury.
    Authors: Roesner JP, Petzelbauer P, Koch A, Mersmann J, Zacharowski PA, Boehm O, Reingruber S, Pasteiner W, Mascher D, Wolzt M, Barthuber C, Noldge-Schomburg GE, Scheeren TW, Zacharowski K
    Crit. Care Med., 2007-07-01;35(7):1730-5.
    Species: Porcine
    Sample Types: Plasma
  32. Macrophage transcriptional responses following in vitro infection with a highly virulent African swine fever virus isolate.
    Authors: Zhang F, Hopwood P, Abrams CC, Downing A, Murray F, Talbot R, Archibald A, Lowden S, Dixon LK
    J. Virol., 2006-11-01;80(21):10514-21.
    Species: Porcine
    Sample Types: Cell Culture Supernates
  33. Complete overlap of interleukin-31 receptor A and oncostatin M receptor beta in the adult dorsal root ganglia with distinct developmental expression patterns.
    Authors: Bando T, Morikawa Y, Komori T, Senba E
    Neuroscience, 2006-08-22;142(4):1263-71.
    Species: Porcine
    Sample Types: Plasma
  34. Effects of therapeutic bronchoalveolar lavage and partial liquid ventilation on meconium-aspirated newborn piglets.
    Authors: Jeng MJ, Soong WJ, Lee YS, Chang HL, Shen CM, Wang CH, Yang SS, Hwang B
    Crit. Care Med., 2006-04-01;34(4):1099-105.
    Species: Porcine
    Sample Types: Serum
  35. Protection against acute porcine lung ischemia/reperfusion injury by systemic preconditioning via hind limb ischemia.
    Authors: Waldow T, Alexiou K, Witt W, Albrecht S, Wagner F, Knaut M, Matschke K
    Transpl. Int., 2005-02-01;18(2):198-205.
    Species: Porcine
    Sample Types: Plasma
  36. Deciphering the involvement of innate immune factors in the development of the host response to PRRSV vaccination.
    Authors: Royaee AR, Husmann RJ, Dawson HD, Calzada-Nova G, Schnitzlein WM, Zuckermann FA, Lunney JK
    Vet. Immunol. Immunopathol., 2004-12-08;102(3):199-216.
    Species: Porcine
    Sample Types: Cell Culture Supernates
  37. Decreased protein accretion in pigs with viral and bacterial pneumonia is associated with increased myostatin expression in muscle.
    Authors: Escobar J, Van Alstine WG, Baker DH
    J. Nutr., 2004-11-01;134(11):3047-53.
    Species: Porcine
    Sample Types: Serum
  38. Vitamin E supplementation does not mitigate the acute morbidity effects of porcine reproductive and respiratory syndrome virus in nursery pigs.
    Authors: Toepfer-Berg TL, Escobar J, Van Alstine WG, Baker DH, Salak-Johnson J, Johnson RW
    J. Anim. Sci., 2004-07-01;82(7):1942-51.
    Species: Porcine
    Sample Types: Serum
  39. Attenuation of reperfusion-induced systemic inflammation by preconditioning with nitric oxide in an in situ porcine model of normothermic lung ischemia.
    Authors: Waldow T, Alexiou K, Witt W, Wagner FM, Gulielmos V, Matschke K, Knaut M
    Chest, 2004-06-01;125(6):2253-9.
    Species: Porcine
    Sample Types: Plasma
  40. The effect of warm humidified CO2 on the dissipation of residual gas following laparoscopy in piglets.
    Authors: Glew PA, Campher MJ, Pearson K, Schofield JC, Davey AK
    J Am Assoc Gynecol Laparosc, 2004-05-01;11(2):204-10.
    Species: Porcine
    Sample Types: Serum
  41. Tumour necrosis factor alpha stimulates nitric oxide production more potently than interleukin-1beta in porcine articular chondrocytes.
    Authors: Goodstone NJ, Hardingham TE
    Rheumatology (Oxford), 2002-08-01;41(8):883-91.
    Species: Porcine
    Sample Types: Cell Culture Supernates

FAQs

  1. Assay Diluent RD1-14 contains visible precipitate. Is this acceptable to use?

    • A small amount of precipitate is expected and acceptable in Assay Diluent RD1-14. Variability has been observed between vials and diluent lots. It may not fully resolubilize but this will not impact assay performance. It is not recommended to filter the precipitate.

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