Porcine IL-1 beta /IL-1F2 Antibody Summary
Ala115-Pro267
Accession # P26889.
Applications
Porcine IL-1 beta /IL-1F2 Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutralization by Porcine IL‑1 beta /IL‑1F2 Antibody. Recombinant Porcine IL-1 beta /IL-1F2 (Catalog # 681-PI) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL-1 beta /IL-1F2 (75 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Porcine IL-1 beta /IL-1F2 Monoclonal Antibody (Catalog # MAB6811). The ND50 is typically 4-15 µg/mL.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-1 beta/IL-1F2
IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 27% amino acid (aa) identity in porcine. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1-4). The porcine IL-1 beta cDNA encodes a 267 aa precursor. A 114 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5, 6). The 17 kDa mature porcine IL-1 beta shares 63-70% aa sequence identity with canine, cotton rat, equine, feline, human, mouse, rat, and rhesus macaque IL-1 beta.
- Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629.
- Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
- Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S.
- Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21.
- Huether, M. et al. (1993) Gene 129:285.
- Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.
Product Datasheets
Citations for Porcine IL-1 beta /IL-1F2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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Development and validation of a multiplex fluorescent microsphere immunoassay assay for detection of porcine cytokines
Authors: SA Hall, SH Ison, C Owles, J Coe, DA Sandercock, AJ Zanella
MethodsX, 2019-05-17;6(0):1218-1227.
Species: Porcine
Sample Types: Tissue Homogenates
Applications: ELISA Capture -
Swine Influenza Virus Induces RIPK1/DRP1-Mediated Interleukin-1 Beta Production.
Authors: Park H, Liu G, Liu Q, Zhou Y
Viruses, 2018-08-09;10(8):.
Species: Porcine
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
NS1 protein of 2009 pandemic influenza A virus inhibits porcine NLRP3 inflammasome-mediated interleukin-1 beta production by suppressing ASC ubiquitination
Authors: HS Park, G Liu, SN Thulasi Ra, SL Landreth, Q Liu, Y Zhou
J. Virol., 2018-03-28;0(0):.
Species: Porcine
Sample Types: Cell Culture Supernates
Applications: ELISA Capture, ELISA Development -
Macrophage transcriptional responses following in vitro infection with a highly virulent African swine fever virus isolate.
Authors: Zhang F, Hopwood P, Abrams CC, Downing A, Murray F, Talbot R, Archibald A, Lowden S, Dixon LK
J. Virol., 2006-11-01;80(21):10514-21.
Species: Porcine
Sample Types: Cell Lysates
Applications: Western Blot -
Simultaneous detection of porcine proinflammatory cytokines using multiplex flow cytometry by the xMAP technology.
Authors: Johannisson A, Jonasson R, Dernfalk J, Jensen-Waern M
Cytometry A, 2006-05-01;69(5):391-5.
Species: Porcine
Sample Types: Serum
Applications: Luminex Development
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We used MAB6811 as the capture antibody for Porcine IL-1b in a sandwich ELISA. Biotinylated AF6911 was used as the detection antibody. The antigen standard was 681-PI. We were able to quantify serum and plasma samples.
