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Rat Anti-Mouse IgG2A APC-conjugated Antibody

Catalog #: F0130
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
F0130
Detection of Mouse IgG2APrimary Antibody by Flow Cytometry.
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Rat Anti-Mouse IgG2A APC-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
The specificity of this antibody was confirmed by direct ELISA on mouse IgG2A monoclonal antibodies, and it does not cross-react with IgG1, IgG2B, IgG3, IgM, IgA, or IgE antibodies. By flow cytometry, this isotype-specific secondary antibody detects primary antibodies of the mouse IgG2A isotype, and does not detect primary antibodies of mouse IgG1, IgG2B, or IgG3 class.
Source
Monoclonal Rat IgG1 Clone # 344701
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Cocktail of mouse IgG1 and IgG2A
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)
Purity
Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Mouse IgG<SUB>2A</SUB>Primary Antibody by Flow Cytometry. View Larger

Detection of Mouse IgG2APrimary Antibody by Flow Cytometry. Human peripheral blood lymphocytes were stained with A) Mouse Anti-Human CD4 Monoclonal Antibody (Catalog # MAB3791, Mouse IgG2A, filled histogram) or isotype control (Catalog # MAB003, open histogram), or B) Mouse Anti-Human CD8a Monoclonal Antibody (Catalog # MAB1509, Mouse IgG2B, filled histogram) or isotype control (Catalog # MAB003, open histogram), followed by APC-conjugated Rat Anti-Mouse IgG2AIsotype-specific Secondary Antibody (Catalog # F0130). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG2A

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G2A Fc
Alternate Names
IgG2A

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Citation for Rat Anti-Mouse IgG2A APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Myeloid-resident neuropilin-1 promotes choroidal neovascularization while mitigating inflammation
    Authors: EMMA Andriessen, F Binet, F Fournier, M Hata, A Dejda, G Mawambo, S Crespo-Gar, F Pilon, M Buscarlet, K Beauchemin, V Bougie, G Cumberlidg, AM Wilson, S Bourgault, FA Rezende, N Beaulieu, JS Delisle, P Sapieha
    Embo Molecular Medicine, 2021-04-19;0(0):e11754.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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