Rat Anti-Mouse IgG2A PerCP-conjugated Antibody

Catalog #: F0131 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
F0131

Detection of Mouse IgG2APrimary Antibody by Flow Cytometry.

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All IgG2A Antibodies

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Rat Anti-Mouse IgG2A PerCP-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

The specificity of this antibody was confirmed by direct ELISA on mouse IgG_2A monoclonal antibodies, and it does not cross-react with IgG₁, IgG_2B, IgG₃, IgM, IgA, or IgE antibodies. By flow cytometry, this isotype-specific secondary antibody detects primary antibodies of the mouse IgG_2A isotype, and does not detect primary antibodies of mouse IgG₁, IgG_2B, or IgG₃ class.

Source

Monoclonal Rat IgG₁ Clone # 344701

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Cocktail of mouse IgG₁ and IgG_2A

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

PerCP (Peridinin-chlorophyll Protein Complex) (Excitation= 482 and 564 nm, Emission= 675 nm)

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Mouse IgG_2APrimary Antibody by Flow Cytometry. Human peripheral blood lymphocytes were stained with A) Mouse Anti-Human CD4 Monoclonal Antibody (Catalog # MAB3791, Mouse IgG_2A, filled histogram) or isotype control (Catalog # MAB003, open histogram), or B) Mouse Anti-Human CD8a Monoclonal Antibody (Catalog # MAB1509, mIgG_2B, filled histogram) or isotype control (Catalog # MAB003, open histogram), followed by PerCP-conjugated Rat Anti-Mouse IgG_2Aisotype-specific secondary antibody (Catalog # F0131). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG2A

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G2A Fc

Alternate Names

IgG2A