Rat Cystatin C Antibody

Catalog #: AF6154 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6154
AF6154-SP

Detection of Rat Cystatin C by Western Blot.

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Rat Cystatin C Antibody Summary

Species Reactivity

Rat

Specificity

Detects rat Cystatin C in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant mouse Cystatin C and approximately 15% cross-reactivity with recombinant human Cystatin C is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat Cystatin C
Met1-Ala140
Accession # P14841

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Simple Western

10 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Rat Cystatin C (Catalog # 6154-PI), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Rat Cystatin C by Western Blot. Western blot shows rat plasma. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Rat Cystatin C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6154) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for Cystatin C at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry

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Cystatin C in Rat Brain. Cystatin C was detected in perfusion fixed frozen sections of rat brain (cerebellum) using Sheep Anti-Rat Cystatin C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6154) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neurons in the molecular layer of the cerebellum. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Simple Western

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Detection of Rat Cystatin C by Simple Western^TM. Simple Western lane view shows rat plasma, loaded at 0.2 mg/mL. A specific band was detected for Cystatin C at approximately 28 kDa (as indicated) using 10 µg/mL of Sheep Anti-Rat Cystatin C Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6154) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cystatin C

Cystatin C is a member of family 2 in the Cystatin superfamily (1). It is involved in processes such as tumor invasion and metastasis, inflammation and some neurological diseases. It inhibits many cysteine proteases such as papain and cathepsins B, H, K, L and S (2, 3). It is ubiquitous in human tissues and body fluids. A point mutation in the gene coding for the 120 amino acid mature Cystatin C causes a hereditary form of amyloid angiopathy in which the protein variant is deposited in the cerebral arteries, leading to fatal cerebral hemorrhage (4). Cystatin C may have additional clinical applications. For example, it is a good marker for glomerular filtration rate (5).

References

Reed, C.H. (2000) British J. Biomed. Sci. 57:323.
Janowski, R. et al. (2001) Nat. Struct. Biol. 8:316.
Abrahamson, M. (1994) Methods Enzymol. 244:685.
Abrahamson, M. et al. (1992) Hum. Genet. 89:377.
Laterza, O.F. et al. (2002) Clin. Chem. 48:699.

Entrez Gene IDs

1471 (Human); 13010 (Mouse); 25307 (Rat)

Alternate Names

ARMD11; bA218C14.4 (cystatin C); CST3; cystatin 3; cystatin C (amyloid angiopathy and cerebral hemorrhage); Cystatin C; cystatin-3; cystatin-C; Gamma-trace; MGC117328; Neuroendocrine basic polypeptide; Post-gamma-globulin