Rat EGF Antibody

Catalog #: MAB3214 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3214
MAB3214-SP

Cell Proliferation Induced by EGF and Neutralization by Rat EGF Antibody.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat EGF Antibody Summary

Species Reactivity

Rat

Specificity

Detects rat EGF in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse (rm) Epigen, rmEpiregulin, recombinant human (rh) HB-EGF, rhEGF, rmEGF, or rhHRG is observed.

Source

Monoclonal Mouse IgG_2B Clone # 420610

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant rat EGF
Asn974-Arg1026
Accession # P07522

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Rat EGF (Catalog # 3214-EG)

Neutralization

Measured by its ability to neutralize EGF-induced proliferation in the Balb/3T3 mouse embryonic fibroblast cell line. The Neutralization Dose (ND₅₀) is typically 0.75-3.75 µg/mL in the presence of 75 pg/mL Recombinant Rat EGF.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Cell Proliferation Induced by EGF and Neutralization by Rat EGF Antibody. Recombinant Rat EGF (Catalog # 3214-EG) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat EGF (75 pg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Rat EGF Monoclonal Antibody (Catalog # MAB3214). The ND₅₀ is typically 0.75-3.75 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EGF

Epidermal growth factor (EGF) is the founding member of the EGF family that also includes TGF-alpha, amphiregulin (AR), betacellulin (BTC), epiregulin (EPR), heparin‑binding EGF-like growth factor (HB-EGF), epigen, and the neuregulins (NRG)-1 through -6 (1). Members of the EGF family share a structural motif, the EGF‑like domain, which is characterized by three intramolecular disulfide bonds that are formed by six similarly spaced conserved cysteine residues (2). All EGF family members are synthesized as type I transmembrane precursor proteins that may contain several EGF domains in the extracellular region. The mature proteins are released from the cell surface by regulated proteolysis (1). The 1133 amino acid (aa) rat EGF precursor contains nine EGF domains and nine LDLR class B repeats. The mature protein consists of 53 aa and is generated by proteolytic excision of the EGF domain proximal to the transmembrane region (3). Mature rat EGF shares 70% and 77% aa sequence identity with mature human and mouse EGF, respectively. EGF is present in various body fluids, including blood, milk, urine, saliva, seminal fluid, pancreatic juice, cerebrospinal fluid, and amniotic fluid (4). Four ErbB (HER) family receptor tyrosine kinases including EGFR/ErbB1, ErbB2, ErbB3 and ErbB4, mediate responses to EGF family members (5). These receptors undergo a complex pattern of ligand induced homo- or hetero-dimerization to transduce EGF family signals (6, 7). EGF binds ErbB1 and depending on the context, induces the formation of homodimers or heterodimers containing ErbB2. Dimerization results in autophosphorylation of the receptor at specific tyrosine residues to create docking sites for a variety of signaling molecules (5, 8). Biological activities ascribed to EGF include epithelial development, angiogenesis, inhibition of gastric acid secretion, fibroblast proliferation, and colony formation of epidermal cells in culture.

References

Harris, R.C. et al. (2003) Exp. Cell Res. 284:2.
Carpenter, G. and Cohen, S. (1990) J. Biol. Chem. 265:7709.
Saggi, S.J. et al. (1992) DNA Cell Biol. 11:481.
Carpenter, G. and Zendegui, J.G. (1986) Exp. Cell Res. 164:1.
Jorissen, R.N. et al. (2003) Exp. Cell Res. 284:31.
Gamett, D.C. et al. (1997) J. Biol. Chem. 272:12052.
Qian, X. et al. (1994) Proc. Natl. Acad. Sci. 91:1500.
Qian, X. et al. (1999) J. Biol. Chem. 274:574.

Long Name

Epidermal Growth Factor

Entrez Gene IDs

1950 (Human); 13645 (Mouse); 25313 (Rat)

Alternate Names

beta-urogastrone; EGF; epidermal growth factor (beta-urogastrone); epidermal growth factor; hEGF; HOMG4; pro-epidermal growth factor; URG; Urogastrone