Home / GFR alpha-1/GDNF R alpha-1 / Rat GFR alpha-1/GDNF R alpha-1 Antibody

Rat GFR alpha-1/GDNF R alpha-1 Antibody

Catalog #: AF560
31 Citations 6 Reviews Datasheet / COA / SDS
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AF560
AF560-SP
Detection of Rat GFR alpha ‑1/GDNF R alpha ‑1 by Western Blot.
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Product Details
Citations (31)
FAQs
Supplemental Products
Reviews (6)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat GFR alpha-1/GDNF R alpha-1 Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat GFR alpha ‑1/GDNF R alpha ‑1 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human GFR alpha ‑1 is observed and less than 1% cross-reactivity with recombinant mouse GFR alpha ‑2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat GFR alpha ‑1/GDNF R alpha ‑1
Asp25-Leu445
Accession # Q62997
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Blockade of Receptor-ligand Interaction
In a functional ELISA, 4-10 µg/mL of this antibody will block 50% of the binding of 4 ng/mL of Recombinant Human GDNF (Catalog # 212-GD) to immobilized Recombinant Rat GFR alpha -1 Fc Chimera (Catalog # 560-GR) coated at 0.5 µg/mL (100 µL/well).
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Rat GFRa-1/GDNF Ra-1 antibody by Western Blot. View Larger

Detection of Rat GFR alpha ‑1/GDNF R alpha ‑1 by Western Blot. Western blot shows lysates of rat brain tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Rat GFRa-1/GDNF Ra-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF560) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for GFRa-1/GDNF Ra-1 at approximately 52 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry GFRa-1/GDNF Ra-1 antibody in Rat Spinal Cord by Immunohistochemistry (IHC-Fr). View Larger

GFR alpha ‑1/GDNF R alpha ‑1 in Rat Spinal Cord. GFRa-1/GDNF Ra-1 was detected in perfusion fixed frozen sections of rat spinal cord using Goat Anti-Rat GFRa-1/GDNF Ra-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF560) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to spinal cord dorsal horn. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GFR alpha-1/GDNF R alpha-1

Glial cell line-derived growth factor (GDNF), neurturin (NTN) and persephin, distant members of the TGF-beta  superfamily, are neurotrophic factors for a variety of neuronal populations in the central and peripheral nervous systems. The bioactivities of GDNF and NTN are mediated through a receptor complex composed of the non ligand-binding signaling subunit (c-Ret receptor tyrosine kinase) and either of two ligand binding subunits (GDNF receptor alpha - (GFR alpha -1) or GFR alpha -2). GFR alpha -1 and
‑2 are members of a family of at least four cysteine-rich glycosyl-phosphatidylinositol (GPI)-linked cell surface proteins that share conserved placements of many of their cysteine residues. Binding of GDNF to membrane-associated GFR alpha -1 or GFR alpha -2 initiates the association with and activation of the Ret tyrosine kinase. Soluble GFR alpha s released enzymatically from the cell surface-associated protein with phosphatidylinositol phospholipase C, as well as recombinantly produced soluble GFR alpha -1, can also bind with high-affinity to GDNF and trigger the activation of Ret tyrosine kinase. Rat GFR alpha -1 cDNA encodes a 468 amino acid (aa) residue protein with an
N‑terminal 24 aa residue hydrophobic signal peptide. Like other GPI-linked proteins, rat GFR alpha -1 has a C-terminal hydrophobic region which is preceded by a three aa residue (ASS) GPI-binding site. Human GFR alpha -1 shares 93% amino acid identity with rat GFR alpha -1. The expression of the various GFR alpha s are differentially regulated in the central and peripheral nervous system, suggesting complementary roles for the GFR alpha s in mediating the activities of the GDNF family of neurotrophic factors.

References
  1. Thompson, J. et al. (1998) Mol. Cell Neurosci. 11:117.
  2. Trupp, M. et al. (1998) Mol. Cell Neurosci. 11:47.
  3. Baloh, R.H. et al. (1998) Proc. Natl. Acad. Sci. USA 95:5801.
Long Name
Glial Cell line-derived Neurotrophic Factor Receptor alpha 1
Entrez Gene IDs
2674 (Human); 14585 (Mouse); 25454 (Rat)
Alternate Names
DKFZp313E1012; DKFZp686J0156; GDNF family receptor alpha 1; GDNF family receptor alpha-1; GDNF R alpha-1; GDNF receptor alpha-1; GDNFRAGFR-ALPHA-1; GDNFR-alpha-1; GDNFRMGC23045; GFR alpha1; GFR alpha-1; GFRA1; GFRa-1; GFR-alpha-1; Glial cell line-derived neurotrophic factor receptor alpha; GPI-linked anchor protein; PI-linked cell-surface accessory protein; RET ligand 1; RET1L; RETL1FLJ10561; TGF-beta related neurotrophic factor receptor 1; TGF-beta-related neurotrophic factor receptor 1; TRNR1FLJ31546

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Citations for Rat GFR alpha-1/GDNF R alpha-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

31 Citations: Showing 1 - 10
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  1. In vitro spermatogenesis in isolated seminiferous tubules of immature mice
    Authors: X Feng, T Matsumura, Y Yamashita, T Sato, K Hashimoto, H Odaka, Y Makino, Y Okada, H Nakamura, H Kimura, T Fujii, T Ogawa
    PLoS ONE, 2023-04-06;18(4):e0283773.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. In vitro spermatogenesis in isolated seminiferous tubules of immature mice
    Authors: X Feng, T Matsumura, Y Yamashita, T Sato, K Hashimoto, H Odaka, Y Makino, Y Okada, H Nakamura, H Kimura, T Fujii, T Ogawa
    PLoS ONE, 2023;18(4):e0283773.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. SOX17-positive rete testis epithelium is required for Sertoli valve formation and normal spermiogenesis in the male mouse
    Authors: A Uchida, K Imaimatsu, H Suzuki, X Han, H Ushioda, M Uemura, K Imura-Kish, R Hiramatsu, HM Takase, Y Hirate, A Ogura, M Kanai-Azum, A Kudo, Y Kanai
    Nature Communications, 2022-12-21;13(1):7860.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. ERR2 and ERR3 promote the development of gamma motor neuron functional properties required for proprioceptive movement control
    Authors: MN Khan, P Cherukuri, F Negro, A Rajput, P Fabrowski, V Bansal, C Lancelin, TI Lee, Y Bian, WP Mayer, T Akay, D Müller, S Bonn, D Farina, T Marquardt
    PloS Biology, 2022-12-21;20(12):e3001923.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  5. Temperature sensitivity of DNA double-strand break repair underpins heat-induced meiotic failure in mouse spermatogenesis
    Authors: Hirano K, Nonami Y, Nakamura Y et al.
    Communications Biology
  6. The netrin-1 receptor UNC5C contributes to the homeostasis of undifferentiated spermatogonia in adult mice
    Authors: V Barroca, C Racine, L Pays, P Fouchet, M Coureuil, I Allemand
    Stem Cell Research, 2022-02-25;60(0):102723.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  7. Proteostasis regulated by testis-specific ribosomal protein RPL39L maintains mouse spermatogenesis.
    Authors: Qianxing Z, Lele Y, Ruona S et al.
    iScience.
  8. Cdc42 is required for male germline niche development in mice
    Authors: Y Mori, S Takashima, M Kanatsu-Sh, Z Yi, T Shinohara
    Cell Reports, 2021-08-17;36(7):109550.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  9. Rubicon prevents autophagic degradation of GATA4 to promote Sertoli cell function
    Authors: T Yamamuro, S Nakamura, Y Yamano, T Endo, K Yanagawa, A Tokumura, T Matsumura, K Kobayashi, H Mori, Y Enokidani, G Yoshida, H Imoto, T Kawabata, M Hamasaki, A Kuma, S Kuribayash, K Takezawa, Y Okada, M Ozawa, S Fukuhara, T Shinohara, M Ikawa, T Yoshimori
    PloS Genetics, 2021-08-05;17(8):e1009688.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. Low retinoic acid levels mediate regionalization of the Sertoli valve in the terminal segment of mouse seminiferous tubules
    Authors: K Imura-Kish, A Uchida, N Tsunekawa, H Suzuki, HM Takase, Y Hirate, M Kanai-Azum, R Hiramatsu, M Kurohmaru, Y Kanai
    Scientific Reports, 2021-01-13;11(1):1110.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. Germ cell depletion in recipient testis has adverse effects on spermatogenesis in orthotopically transplanted testis pieces via retinoic acid insufficiency
    Authors: A Tsuchimoto, M Tone, N Ogonuki, M Hada, A Ogura, S Takashima
    Sci Rep, 2020-07-01;10(1):10796.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  12. GDNF and GFRalpha1 Are Required for Proper Integration of Adult-Born Hippocampal Neurons
    Authors: A Bonafina, MF Trinchero, AS Ríos, P Bekinschte, AF Schinder, G Paratcha, F Ledda
    Cell Rep, 2019-12-24;29(13):4308-4319.e4.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  13. PLZFposc-KITpos-delineated A1-A4-differentiating spermatogonia by subset and stage detection upon Bouin fixation
    Authors: RL Tang, LQ Fan
    Asian J. Androl., 2019-05-01;21(3):309-318.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  14. The Mammalian Spermatogenesis Single-Cell Transcriptome, from Spermatogonial Stem Cells to Spermatids
    Authors: BP Hermann, K Cheng, A Singh, L Roa-De La, KN Mutoji, IC Chen, H Gilderslee, JD Lehle, M Mayo, B Westernstr, NC Law, MJ Oatley, EK Velte, BA Niedenberg, D Fritze, S Silber, CB Geyer, JM Oatley, JR McCarrey
    Cell Rep, 2018-11-06;25(6):1650-1667.e8.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  15. The adult human testis transcriptional cell atlas
    Authors: J Guo, EJ Grow, H Mlcochova, GJ Maher, C Lindskog, X Nie, Y Guo, Y Takei, J Yun, L Cai, R Kim, DT Carrell, A Goriely, JM Hotaling, BR Cairns
    Cell Res., 2018-10-12;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  16. Prolyl isomerase Pin1 is required sperm production by promoting mitosis progression of spermatogonial stem cells
    Authors: A Kurita-Suz, Y Kamo, C Uchida, K Tanemura, K Hara, T Uchida
    Biochem. Biophys. Res. Commun., 2018-02-10;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Immunochromatography
  17. Advanced immunostaining approaches to study early male germ cell development
    Authors: BA Niedenberg, CB Geyer
    Stem Cell Res, 2018-02-09;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  18. Aging-related limit of exercise efficacy on motor decline
    Authors: JC Arnold, MA Cantu, EA Kasanga, VA Nejtek, EV Papa, N Bugnariu, MF Salvatore
    PLoS ONE, 2017-11-27;12(11):e0188538.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  19. Novel combinatorial screening identifies neurotrophic factors for selective classes of motor neurons
    Authors: S Schaller, D Buttigieg, A Alory, A Jacquier, M Barad, M Merchant, D Gentien, P de la Gran, G Haase
    Proc. Natl. Acad. Sci. U.S.A, 2017-03-07;114(12):E2486-E2493.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  20. Transcriptome analysis reveals transmembrane targets on transplantable midbrain dopamine progenitors.
    Authors: Bye C, Jonsson M, Bjorklund A, Parish C, Thompson L
    Proc Natl Acad Sci U S A, 2015-03-09;112(15):E1946-55.
    Species: Mouse, Rat
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: Flow Cytometry, IHC
  21. PAX7 expression defines germline stem cells in the adult testis.
    Authors: Aloisio G, Nakada Y, Saatcioglu H, Pena C, Baker M, Tarnawa E, Mukherjee J, Manjunath H, Bugde A, Sengupta A, Amatruda J, Cuevas I, Hamra F, Castrillon D
    J Clin Invest, 2014-08-18;124(9):3929-44.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  22. LIN28A marks the spermatogonial progenitor population and regulates its cyclic expansion.
    Authors: Chakraborty P, Buaas F, Sharma M, Snyder E, de Rooij D, Braun R
    Stem Cells, 2014-04-01;32(4):860-73.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  23. Glial cell line-derived neurotrophic factor (GDNF) induces neuritogenesis in the cochlear spiral ganglion via neural cell adhesion molecule (NCAM).
    Authors: Euteneuer S, Yang K, Chavez E, Leichtle A, Loers G, Olshansky A, Pak K, Schachner M, Ryan A
    Mol Cell Neurosci, 2012-12-20;54(0):30-43.
    Species: Rat
    Sample Types:
    Applications: Western Blot
  24. Retinoic acid availability drives the asynchronous initiation of spermatogonial differentiation in the mouse.
    Authors: Snyder EM, Small C, Griswold MD
    Biol. Reprod., 2010-07-21;83(5):783-90.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  25. The heterogeneity of spermatogonia is revealed by their topology and expression of marker proteins including the germ cell-specific proteins Nanos2 and Nanos3.
    Authors: Suzuki H, Sada A, Yoshida S, Saga Y
    Dev. Biol., 2009-10-08;336(2):222-31.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  26. Glial cell line-derived neurotrophic factor: an intraovarian factor that enhances oocyte developmental competence in vitro.
    Authors: Linher K, Wu D, Li J
    Endocrinology, 2007-05-31;148(9):4292-301.
    Species: Porcine
    Sample Types: Cell Lysates, Whole Cells
    Applications: Neutralization, Western Blot
  27. Astrocyte and muscle-derived secreted factors differentially regulate motoneuron survival.
    Authors: Taylor AR, Gifondorwa DJ, Newbern JM, Robinson MB, Strupe JL, Prevette D, Oppenheim RW, Milligan CE
    J. Neurosci., 2007-01-17;27(3):634-44.
    Species: Chicken
    Sample Types: Whole Cells
    Applications: Neutralization
  28. Involvement of GDNF and its receptors in the maturation of the periodontal Ruffini endings.
    Authors: Igarashi Y, Aita M, Suzuki A, Nandasena T, Kawano Y, Nozawa-Inoue K, Maeda T
    Neurosci. Lett., 2006-12-18;412(3):222-6.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  29. Glial cell line-derived neurotrophic factor family members sensitize nociceptors in vitro and produce thermal hyperalgesia in vivo.
    Authors: Malin SA, Molliver DC, Koerber HR, Cornuet P, Frye R, Albers KM, Davis BM
    J. Neurosci., 2006-08-16;26(33):8588-99.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: ICC, IHC-Fr
  30. Nerve growth factor, glial cell line-derived neurotrophic factor and neurturin prevent semaphorin 3A-mediated growth cone collapse in adult sensory neurons.
    Authors: Wanigasekara Y, Keast JR
    Neuroscience, 2006-07-28;142(2):369-79.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  31. Neurotrophin and GDNF family ligands promote survival and alter excitotoxic vulnerability of neurons derived from murine embryonic stem cells.
    Authors: Lee CS, Tee LY, Dusenbery S, Takata T, Golden JP, Pierchala BA, Gottlieb DI, Johnson EM, Choi DW, Snider BJ
    Exp. Neurol., 2005-01-01;191(1):65-76.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC

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Rat GFR alpha-1/GDNF R alpha-1 Antibody
By Anonymous on 07/22/2021
Application: IHC Sample Tested: Testis tissue Species: Rat
Immunohistochemistry Rat GFR alpha-1/GDNF R alpha-1 Antibody AF560
Rat GFR alpha-1/GDNF R alpha-1 Antibody
By Anonymous on 04/27/2021
Application: IHC Sample Tested: Adult brain Species: Rat
Immunohistochemistry Rat GFR alpha-1/GDNF R alpha-1 Antibody AF560
Rat GFR alpha-1/GDNF R alpha-1 Antibody
By Anonymous on 04/13/2021
Application: ICC/IF Sample Tested: Balb/3T3 mouse embryonic fibroblast cell line Species: Mouse
Immunocytochemistry/Immunofluorescence Rat GFR alpha-1/GDNF R alpha-1 Antibody AF560
Rat GFR alpha-1/GDNF R alpha-1 Antibody
By Anonymous on 11/11/2019
Application: ICC/IF Sample Tested: Adult testis Species: Mouse

IF 1:200 detect gfra1 positive cell in testis

Immunocytochemistry/Immunofluorescence Rat GFR alpha-1/GDNF R alpha-1 Antibody AF560
Rat GFR alpha-1/GDNF R alpha-1 Antibody
By Anonymous on 09/12/2019
Application: ICC/IF Sample Tested: spermatogonial stem cells Species: Mouse
Immunocytochemistry/Immunofluorescence Rat GFR alpha-1/GDNF R alpha-1 Antibody AF560
Rat GFR alpha ‑1/GDNF R alpha ‑1 Antibody
By Anonymous on 04/22/2016
Application: ICC/IF Sample Tested: Testis tissue Species: Mouse

Frozen sections from paraformaldehyde fixed postnatal day 1 mouse testes were cut at 5 microns and affixed to sides. Sections were permealbilized with 0.1% Triton X-100 in PBS, then blocked with 3% BSA in 0.1% Triton X-100 in PBS for 30 min. Sections were then incubated for 1 hour at RT with Goat Anti-Rat GFR alpha 1/GDNF R alpha 1 Antibody (R&D Systems AF560) at 5 µg/ml. After washing 3X with 0.1% Triton X-100 in PBS, sections were incubated with Donkey anti-Goat (H+L) Secondary Antibody, Alexa Fluor 555 conjugate (red; ThermoFisher Scientific A21432) for 1 hour at RT. Phalloidin was used a counterstain (blue) to label F-actin.

Immunocytochemistry/Immunofluorescence Rat GFR alpha ‑1/GDNF R alpha ‑1 Antibody AF560