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Rat Glut4 Antibody

Catalog #: MAB1262
13 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1262
MAB1262-SP
Glut4 in L6 Rat Cell Line.
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Product Details
Citations (13)
FAQs
Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat Glut4 Antibody Summary

Species Reactivity
Rat
Specificity
Recognizes an epitope in the cytoplasmic portion of rat Glut4 and has been shown to bind only to the muscle-adipose isoform of the glucose transporter, which is antigenically unique from the other transport proteins (1-3). Recognizes Glut4 in human, monkey, rat, mouse, and rabbit systems. It does not recognize this protein in canine systems. Its ability to bind to glucose transporter from other species has not been tested.
Source
Monoclonal Mouse IgG1 Clone # 1F8
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Partially purified vesicles containing Glut4
Formulation
Supplied as a 0.2 um filtered solution in PBS. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
Zorzano, et al. (1989) J. Biol. Chem. 264:12358.
 
Immunohistochemistry
5-25 µg/mL
See below
Immunoprecipitation
Fischer, et al. (1997) J. Biol. Chem. 272:7085.
 
Immunoaffinity Purification
Membrane vesicles from fat and muscle were immunoadsorbed using this antibody linked to sepharose (3, 4).
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Glut4 antibody in L6 Rat Cell Line by Immunocytochemistry (ICC). View Larger

Glut4 in L6 Rat Cell Line. Glut4 was detected in immersion fixed L6 rat myoblast cell line (differentiated to muscle) using 10 µg/mL Rat Glut4 Monoclonal Antibody (Catalog # MAB1262) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry Glut4 antibody in Rat Heart Tissue by Immunohistochemistry (IHC-P). View Larger

Glut4 in Rat Heart Tissue. Glut4 was detected in immersion fixed paraffin-embedded sections of rat heart tissue using Mouse Anti-Rat Glut4 Monoclonal Antibody (Catalog # MAB1262) at 5 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (brown; Catalog # VC001) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cardiomyocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C, as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.

Background: Glut4

Glut4 belongs to the facilitative glucose transporter protein family that comprises 13 members. It is an integral membrane protein with 12 transmembrane domains. Glut4 is expressed in the insulin-responsive tissues, muscle and adipose tissue. Insulin stimulation leads to translocation of Glut4 from intracellular stores to the cell surface resulting in the enhancement of glucose uptake.

References
  1. James, et al. (1988) Nature 333:183.
  2. Fukumoto, et al. (1989) J. Biol. Chem. 264:7776.
  3. Zorzano, et al. (1989) J. Biol. Chem. 264:12358.
  4. Rodnick, et al. (1992) J. Biol. Chem. 267:6278.
Entrez Gene IDs
6517 (Human); 20528 (Mouse); 25139 (Rat)
Alternate Names
Glut4; GLUT-4; GLUT4Glucose transporter type 4, insulin-responsive; insulin-responsive glucose transporter type 4; SLC2A4; solute carrier family 2 (facilitated glucose transporter), member 4; solute carrier family 2, facilitated glucose transporter member 4

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Citations for Rat Glut4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. The Parkinson's disease-linked Leucine-rich repeat kinase 2 (LRRK2) is required for insulin-stimulated translocation of GLUT4
    Authors: N Funk, M Munz, T Ott, K Brockmann, A Wenninger-, R Kühn, D Vogt-Weise, F Giesert, W Wurst, T Gasser, S Biskup
    Sci Rep, 2019-03-14;9(1):4515.
    Species: Human, Rat
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  2. 3D organoid-derived human glomeruli for personalised podocyte disease modelling and drug screening
    Authors: LJ Hale, SE Howden, B Phipson, A Lonsdale, PX Er, I Ghobrial, S Hosawi, S Wilson, KT Lawlor, S Khan, A Oshlack, C Quinlan, R Lennon, MH Little
    Nat Commun, 2018-12-04;9(1):5167.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  3. Changes in photoperiod alter Glut4 expression in skeletal muscle of C57BL/6J mice
    Authors: A Tashiro, S Shibata, Y Takai, T Uchiwa, M Furuse, S Yasuo
    Biochem. Biophys. Res. Commun, 2017-02-09;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  4. HDL and Glut1 inhibition reverse a hypermetabolic state in mouse models of myeloproliferative disorders.
    Authors: Gautier E, Westerterp M, Bhagwat N, Cremers S, Shih A, Abdel-Wahab O, Lutjohann D, Randolph G, Levine R, Tall A, Yvan-Charvet L
    J Exp Med, 2013-01-14;210(2):339-53.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  5. E4orf1: a novel ligand that improves glucose disposal in cell culture.
    Authors: Dhurandhar EJ, Dubuisson O, Mashtalir N
    PLoS ONE, 2011-08-23;6(8):e23394.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Nuclear factor-kappaB decoy oligodeoxynucleotides ameliorate impaired glucose tolerance and insulin resistance in mice with cecal ligation and puncture-induced sepsis.
    Authors: Matsuda N, Yamamoto S, Yokoo H, Tobe K, Hattori Y
    Crit. Care Med., 2009-10-01;37(10):2791-9.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  7. A transgenic mouse model to study glucose transporter 4myc regulation in skeletal muscle.
    Authors: Schertzer JD, Antonescu CN, Bilan PJ, Jain S, Huang X, Liu Z, Bonen A, Klip A
    Endocrinology, 2008-12-12;150(4):1935-40.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Immunoprecipitation, Western Blot
  8. DOC2B: a novel syntaxin-4 binding protein mediating insulin-regulated GLUT4 vesicle fusion in adipocytes.
    Authors: Fukuda N, Emoto M, Nakamori Y, Taguchi A, Miyamoto S, Uraki S, Oka Y, Tanizawa Y
    Diabetes, 2008-11-25;58(2):377-84.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  9. Deficiency and inhibition of cathepsin K reduce body weight gain and increase glucose metabolism in mice.
    Authors: Yang M, Sun J, Zhang T, Liu J, Zhang J, Shi MA, Darakhshan F, Guerre-Millo M, Clement K, Gelb BD, Dolgnov G, Shi GP
    Arterioscler. Thromb. Vasc. Biol., 2008-09-25;28(12):2202-8.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  10. The human glomerular podocyte is a novel target for insulin action.
    Authors: Coward RJ, Welsh GI, Yang J, Tasman C, Lennon R, Koziell A, Satchell S, Holman GD, Kerjaschki D, Tavare JM, Mathieson PW, Saleem MA
    Diabetes, 2005-11-01;54(11):3095-102.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  11. Effects of hormone therapy on insulin signaling proteins in skeletal muscle of cynomolgus monkeys.
    Authors: Shadoan MK, Zhang L, Wagner JD
    Steroids, 2004-05-01;69(5):313-8.
    Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  12. Ischemic preconditioning activates AMPK in a PKC-dependent manner and induces GLUT4 up-regulation in the late phase of cardioprotection.
    Authors: Nishino Y, Miura T, Miki T, Sakamoto J, Nakamura Y, Ikeda Y, Kobayashi H, Shimamoto K
    Cardiovasc. Res., 2004-02-15;61(3):610-9.
    Species: Rabbit
    Sample Types: Whole Tissue
    Applications: IHC-P
  13. Requirement for 3-phosphoinositide-kependent dinase-1 (PDK-1) in insulin-induced glucose uptake in immortalized brown adipocytes.
    Authors: Sakaue H, Nishizawa A, Ogawa W, Teshigawara K, Mori T, Takashima Y, Noda T, Kasuga M
    J. Biol. Chem., 2003-07-10;278(40):38870-4.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC

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Rat Glut4 Antibody
By Anonymous on 09/01/2021
Application: IHC Sample Tested: Skeletal muscle Species: Rat
Immunohistochemistry Rat Glut4 Antibody MAB1262