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Rat Growth Hormone Antibody

Catalog #: AF1566
5 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1566
AF1566-SP
Growth Hormone in Rat Pituitary.
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Citations (5)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat Growth Hormone Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat Growth Hormone in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human Growth Hormone is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant rat Growth Hormone
Leu27-Phe216
Accession # P01244
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Purity
Immunogen affinity purified

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Rat Growth Hormone
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Growth Hormone antibody in Rat Pituitary by Immunohistochemistry (IHC-Fr). View Larger

Growth Hormone in Rat Pituitary. Growth Hormone was detected in perfusion fixed frozen sections of rat pituitary using Goat Anti-Rat Growth Hormone Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1566) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Growth Hormone

Growth Hormone, also named somatotropin, belongs to the somatotropin/prolactin superfamily. It is synthesized in the anterior pituitary gland. Growth Hormone regulates body growth by stimulating the secretion of IGF-I by liver and other tissues.

Entrez Gene IDs
2688 (Human)
Alternate Names
GH1; GHB5; GHIGHD1B; GHN; GH-N; GHNGrowth hormone; GH-Nsomatotropin; growth hormone 1Pituitary growth hormone; Growth Hormone; hGH-N; IGHD1A; IGHD2; Somatotropin

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Citations for Rat Growth Hormone Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. A new method for physical disector analyses of numbers and mean volumes of immunohistochemically labelled cells in paraffin sections
    Authors: I Hofmann, E Kemter, S Fiedler, N Theobalt, L Fonteyne, E Wolf, R Wanke, A Blutke
    Journal of neuroscience methods, 2021-07-01;361(0):109272.
    Species: Porcine
    Sample Types: Whole Tissue
    Applications: IHC
  2. OXR1A, a Coactivator of PRMT5 Regulating Histone Arginine Methylation
    Authors: M Yang, X Lin, F Segers, R Suganthan, GA Hildrestra, JE Rinholm, PA Aas, MML Sousa, S Holm, N Bolstad, D Warren, RK Berge, RF Johansen, A Yndestad, E Kristianse, A Klungland, L Luna, L Eide, B Halvorsen, P Aukrust, M Bjørås
    Cell Rep, 2020-03-24;30(12):4165-4178.e7.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. Chemokine CXCL14-like immunoreactivity in the &alphaMSH-producing cells and PRL-producing cells of the flat-tailed house gecko pituitary
    Authors: H Suzuki, T Yamamoto
    J. Vet. Med. Sci., 2020-02-10;82(4):408-413.
    Species: Gecko
    Sample Types: Whole Tissue
    Applications: IF
  4. Wnt signaling in estrogen-induced lactotroph proliferation.
    Authors: Giles A, Madec F, Friedrichsen S, Featherstone K, Chambers T, Harper CV, Resch J, Brabant G, Davis JR
    J. Cell. Sci., 2011-01-18;124(0):540-7.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-P
  5. Traumatic brain injury causes long-term reduction in serum growth hormone and persistent astrocytosis in the cortico-hypothalamo-pituitary axis of adult male rats.
    Authors: Kasturi BS, Stein DG
    J. Neurotrauma, 2009-08-01;26(8):1315-24.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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