Rat IgG2A Allophycocyanin Isotype Control Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Rat IgG Control by Flow Cytometry J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Rat Anti-Mouse GM-CSF Ra APC-conjugated Monoclonal Antibody (Catalog # FAB6130A, filled histogram) or Rat IgG APC-conjugated Isotype Control Antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Reconstitution Calculator
Preparation and Storage
Background: IgG2A
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.
Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.
Product Datasheets
Citations for Rat IgG2A Allophycocyanin Isotype Control
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Biodistribution and Cellular Internalization of Inactivated SARS-CoV-2 in Wild-Type Mice
Authors: A Hudák, G Morgan, J Bacovsky, R Patai, TF Polgár, A Letoha, A Pettko-Sza, C Vizler, L Szilák, T Letoha
International Journal of Molecular Sciences, 2022-07-09;23(14):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry Control -
Role of CCR3 in respiratory syncytial virus infection of airway epithelial cells.
Authors: Wellemans V, Benhassou H, Fuselier E, Bellesort F, Dury S, Lebargy F, Dormoy V, Fichel C, Naour R, Gounni A, Lamkhioued B
iScience, 2021-11-14;24(12):103433.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry Control -
The gut microbiota induces Peyer's-patch-dependent secretion of maternal IgA into milk
Authors: K Usami, K Niimi, A Matsuo, Y Suyama, Y Sakai, S Sato, K Fujihashi, H Kiyono, S Uchino, M Furukawa, J Islam, K Ito, T Moriya, Y Kusumoto, M Tomura, RC Hovey, J Sugawara, H Yoneyama, H Kitazawa, K Watanabe, H Aso, T Nochi
Cell Reports, 2021-09-07;36(10):109655.
Species: Transgenic Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Impaired lymphocyte trafficking in mice deficient in the kinase activity of PKN1
Authors: R Mashud, A Nomachi, A Hayakawa, K Kubouchi, S Danno, T Hirata, K Matsuo, T Nakayama, R Satoh, R Sugiura, M Abe, K Sakimura, S Wakana, H Ohsaki, S Kamoshida, H Mukai
Sci Rep, 2017-08-09;7(1):7663.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
MafB enhances the phagocytic activity of RAW264.7 macrophages by promoting Fcgr3 expression
Authors: Isao Kubota
Biochem. Biophys. Res. Commun., 2016-11-12;0(0):.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
NKP30-B7-H6 Interaction Aggravates Hepatocyte Damage through Up-Regulation of Interleukin-32 Expression in Hepatitis B Virus-Related Acute-On-Chronic Liver Failure.
Authors: Zou Y, Bao J, Pan X, Lu Y, Liao S, Wang X, Wang G, Lin D
PLoS ONE, 2015-08-04;10(8):e0134568.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Neuronal transgene expression in dominant-negative SNARE mice.
Authors: Fujita T, Chen M, Li B, Smith N, Peng W, Sun W, Toner M, Kress B, Wang L, Benraiss A, Takano T, Wang S, Nedergaard M
J Neurosci, 2014-12-10;34(50):16594-604.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry Control -
The developmental pathway for CD103(+)CD8+ tissue-resident memory T cells of skin.
Authors: Mackay L, Rahimpour A, Ma J, Collins N, Stock A, Hafon M, Vega-Ramos J, Lauzurica P, Mueller S, Stefanovic T, Tscharke D, Heath W, Inouye M, Carbone F, Gebhardt T
Nat Immunol, 2013-10-27;14(12):1294-301.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry Control -
CXCL9 causes heterologous desensitization of CXCL12-mediated memory T lymphocyte activation.
Authors: Giegold O, Ogrissek N, Richter C, Schroder M, Herrero San Juan M, Pfeilschifter J, Radeke H
J Immunol, 2013-02-27;190(7):3696-705.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Steady-state generation of mucosal IgA+ plasmablasts is not abrogated by B-cell depletion therapy with rituximab.
Authors: Mei HE, Frolich D, Giesecke C, Loddenkemper C, Reiter K, Schmidt S, Feist E, Daridon C, Tony HP, Radbruch A, Dorner T
Blood, 2010-09-09;116(24):5181-90.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry Control
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Freshly isolated and 48Hrs stimulated human PBMCs using anti-human CD3 were stained with the Rat IgG2A Ab Isotype and TIM-3 Ab with similar background. The isotype control has been very consistent between runs and helps decide where the gated should be started for the test antibody.
