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Rat IgG2B APC‑conjugated Isotype Control

Catalog #: IC013A
7 Citations Datasheet / COA / SDS
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IC013A
Detection of Rat IgG Isotype Control by Flow Cytometry
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Product Details
Citations (7)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat IgG2B APC‑conjugated Isotype Control Summary

Specificity
Selected for use as a rat IgG2B isotype control for flow cytometry applications.
Source
Monoclonal Rat IgG2B Clone # 141945
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
KLH
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Control
Negative control for use in conjunction with R&D Systems antibodies. This isotype control has been derivatized with a quantity of fluorochrome that matched the F/P (fluorochrome/protein) ratio of R&D Systems monoclonal reagents. The recommended concentration is 10 μL/106 cells.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Control Detection of Rat IgG Isotype Control by Flow Cytometry View Larger

Detection of Rat IgG Control by Flow Cytometry Mouse splenocytes treated with 1 µg/mL Concanavalin A for 3 days were stained with Rat Anti-Mouse ICOS APC-conjugated Monoclonal Antibody (Catalog # FAB168A, filled histogram) or Rat IgG APC-conjugated Isotype Control Antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IgG2B

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G2B Fc
Alternate Names
IgG2B

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Citations for Rat IgG2B APC‑conjugated Isotype Control

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Distinct myeloid population phenotypes dependent on TREM2 expression levels shape the pathology of traumatic versus demyelinating CNS disorders
    Authors: Gao, H;Di, J;Clausen, BH;Wang, N;Zhu, X;Zhao, T;Chang, Y;Pang, M;Yang, Y;He, R;Wang, Y;Zhang, L;Liu, B;Qiu, W;Lambertsen, KL;Brambilla, R;Rong, L;
    Cell reports
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Targeting TREM2 on tumor-associated macrophages enhances immunotherapy
    Authors: M Binnewies, JL Pollack, J Rudolph, S Dash, M Abushawish, T Lee, NS Jahchan, P Canaday, E Lu, M Norng, S Mankikar, VM Liu, X Du, A Chen, R Mehta, R Palmer, V Juric, L Liang, KP Baker, L Reyno, MF Krummel, M Streuli, V Sriram
    Cell Reports, 2021-10-19;37(3):109844.
    Species: Human
    Sample Types: Whole Cell
    Applications: Flow Cytometry
  3. Antibiotic-induced dysbiosis of gut microbiota impairs corneal development in postnatal mice by affecting CCR2 negative macrophage distribution
    Authors: Mingjuan Wu, Jun Liu, Fanying Li, Shuoya Huang, Jingxin He, Yunxia Xue et al.
    Mucosal Immunology
  4. Myeloid cells that impair immunotherapy are restored in melanomas which acquire resistance to BRAF inhibitors
    Authors: SM Steinberg, T Shabaneh, P Zhang, V Martyanov, Z Li, B Malik, T Wood, A Boni, A Molodtsov, CV Angeles, TJ Curiel, M Whitfield, MJ Turk
    Cancer Res, 2017-02-15;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Targeted inhibition of T-cell factor activity promotes syndecan-2 expression and sensitization to doxorubicin in osteosarcoma cells and bone tumors in mice.
    J. Bone Miner. Res., 2012-10-01;27(10):2118-29.
    Species: Human
    Sample Types: Whole Cells
    Applications: Control
  6. Targeting Stat3 induces senescence in tumor cells and elicits prophylactic and therapeutic immune responses against breast cancer growth mediated by NK cells and CD4+ T cells.
    J. Immunol., 2012-06-29;189(3):1162-72.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Control
  7. Chemokine responsiveness of CD4+ CD25+ regulatory and CD4+ CD25- T cells from atopic and nonatopic donors.
    Authors: Ahern D, Lloyd CM, Robinson DS
    Allergy, 2009-02-07;64(8):1121-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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