Rat IL-1 beta /IL-1F2 Antibody Summary
Val117-Ser268
Accession # Q63264
Applications
Rat IL-1 beta /IL-1F2 Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Cell Proliferation Induced by IL‑1 beta /IL‑1F2 and Neutralization by Rat IL‑1 beta /IL‑1F2 Antibody. Recombinant Rat IL-1 beta /IL-1F2 (Catalog # 501-RL) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL-1 beta /IL-1F2 (10 ng/mL) is neutralized (green line) by increasing concentrations of Rat IL-1 beta /IL-1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-501-NA). The ND50 is typically 2-10 µg/mL.
IL‑1 beta /IL‑1F2 in Rat Splenocytes. IL‑1 beta /IL‑1F2 was detected in immersion fixed LPS-stimulated rat splenocytes using 5 µg/mL Rat IL‑1 beta /IL‑1F2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑501‑NA) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-1 beta/IL-1F2
IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2, IL1B), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 26% amino acid (aa) identity in rat. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI. Intracellular cleavage of the IL-1 beta precursor by Caspase-1/ICE is a key step in the inflammatory response. The 17 kDa molecular weight mature rat IL-1 beta shares 90% aa sequence identity with cotton rat and mouse and 67%-78% with canine, equine, feline, human, porcine, and rhesus macaque IL-1 beta. IL-1 beta functions in a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. IL-1 beta dysregulation is implicated in many pathological conditions including sepsis, rheumatoid arthritis, inflammatory bowel disease, acute and chronic myelogenous leukemia, insulin-dependent diabetes mellitus, atherosclerosis, neuronal injury, and aging-related diseases.
Product Datasheets
Citations for Rat IL-1 beta /IL-1F2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
46
Citations: Showing 1 - 10
Filter your results:
Filter by:
-
Disulfiram Ophthalmic Solution Inhibited Macrophage Infiltration by Suppressing Macrophage Pseudopodia Formation in a Rat Corneal Alkali Burn Model
Authors: T Ikebukuro, T Arima, M Kasamatsu, Y Nakano, Y Tobita, M Uchiyama, Y Terashima, E Toda, A Shimizu, H Takahashi
International Journal of Molecular Sciences, 2023-01-01;24(1):.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
Microglia activation and inflammation in hippocampus attenuates memory and mood functions during experimentally induced diabetes in rat
Authors: A Nagayach, R Bhaskar, I Patro
Journal of chemical neuroanatomy, 2022-09-08;0(0):102160.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
Multiple therapeutic effects of human neural stem cells derived from induced pluripotent stem cells in a rat model of post-traumatic syringomyelia
Authors: T Xu, X Li, Y Guo, E Uhlin, L Holmberg, S Mitra, D Winn, A Falk, E Sundström
EBioMedicine, 2022-02-16;77(0):103882.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
Enhancing fractalkine/CX3CR1 signalling pathway can reduce neuroinflammation by attenuating microglia activation in experimental diabetic retinopathy
Authors: M Jiang, H Xie, C Zhang, T Wang, H Tian, L Lu, JY Xu, GT Xu, L Liu, J Zhang
Journal of Cellular and Molecular Medicine, 2022-01-11;0(0):.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot -
Neuronal ApoE4 stimulates C/EBPbeta activation, promoting Alzheimer's disease pathology in a mouse model
Authors: ZH Wang, Y Xia, Z Wu, SS Kang, JC Zhang, P Liu, X Liu, W Song, V Huin, CM Dhaenens, SP Yu, XC Wang, K Ye
Progress in neurobiology, 2021-12-24;209(0):102212.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
The selective estrogen receptor modulator tamoxifen protects against subtle cognitive decline and early markers of injury 24�h after hippocampal silent infarct in male Sprague-Dawley rats
Authors: CA Finney, A Shvetcov, RF Westbrook, MJ Morris, NM Jones
Hormones and behavior, 2021-07-06;134(0):105016.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
ApoE4 activates C/EBP&beta/&delta-secretase with 27-hydroxycholesterol, driving the pathogenesis of Alzheimer's disease
Authors: ZH Wang, Y Xia, P Liu, X Liu, L Edgington-, K Lei, SP Yu, XC Wang, K Ye
Progress in neurobiology, 2021-03-11;0(0):102032.
Species: Rat
Sample Types: Cell Lysates, Whole Cells
Applications: ICC, Neutralization, Western Blot -
CCR5 signaling promotes lipopolysaccharide-induced macrophage recruitment and alveolar developmental arrest
Authors: Z Chen, X Xie, N Jiang, J Li, L Shen, Y Zhang
Cell Death & Disease, 2021-02-15;12(2):184.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
Supplementation with Fermented Rice Bran Attenuates Muscle Atrophy in a Diabetic Rat Model
Authors: TB Rusbana, AZ Agista, WD Saputra, Y Ohsaki, K Watanabe, A Ardiansyah, S Budijanto, T Koseki, H Aso, M Komai, H Shirakawa
Nutrients, 2020-08-12;12(8):.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot -
Of Mice and Monkeys: Neuroprotective Efficacy of the p38 Inhibitor BIRB 796 Depends on Model Duration in Experimental Glaucoma
Authors: WS Lambert, S Pasini, JW Collyer, CR Formichell, P Ghose, BJ Carlson, DJ Calkins
Sci Rep, 2020-05-22;10(1):8535.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
Microglial activation by microbial neuraminidase through TLR2 and TLR4 receptors
Authors: MDM Fernández-, JM Grondona, P Fernández-, MD López-Ával
J Neuroinflammation, 2019-12-02;16(1):245.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Microglia-Triggered Plasticity of Intrinsic Excitability Modulates Psychomotor Behaviors in Acute Cerebellar Inflammation
Authors: M Yamamoto, M Kim, H Imai, Y Itakura, G Ohtsuki
Cell Rep, 2019-09-10;28(11):2923-2938.e8.
Species: Rat
Sample Types: Tissue Culture Supernates
Applications: Western Blot -
Deficiency in BDNF/TrkB Neurotrophic Activity Stimulates δ-Secretase by Upregulating C/EBP? in Alzheimer's Disease
Authors: ZH Wang, J Xiang, X Liu, SP Yu, FP Manfredsso, IM Sandoval, S Wu, JZ Wang, K Ye
Cell Rep, 2019-07-16;28(3):655-669.e5.
Species: Rat
Sample Types: Whole Cells
Applications: Neutralization -
Andrographolide relieved pathological pain generated by spared nerve injury model in mice
Authors: HC Wang, HS Tsay, HN Shih, YA Chen, KM Chang, DC Agrawal, S Huang, YL Lin, MJ Lee
Pharm Biol, 2018-12-01;56(1):124-131.
Species: Mouse
Sample Types: Whole Cells
Applications: IHC -
Baicalein attenuates neuropathic pain and improves sciatic nerve function recovery in rats with partial sciatic nerve transection
Authors: HC Lai, CH Lu, CS Wong, BF Lin, SM Chan, CY Kuo, ZF Wu
J Chin Med Assoc, 2018-08-20;0(0):.
Species: Rat
Sample Types: Whole Tissue
-
Anti-Inflammatory Action of Sitagliptin and Linagliptin in Doxorubicin Nephropathy
Authors: CH Jo, S Kim, JS Park, GH Kim
Kidney Blood Press. Res., 2018-06-15;43(3):987-999.
Species: Rat
Sample Types: Tissue Homogenates
Applications: ELISA Development (Capture) -
Glial interleukin-1? upregulates neuronal sodium channel 1.7 in trigeminal ganglion contributing to temporomandibular joint inflammatory hypernociception in rats
Authors: P Zhang, RY Bi, YH Gan
J Neuroinflammation, 2018-04-20;15(1):117.
Species: Rat
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC, Western Blot -
Hyperammonemia alters membrane expression of GluA1 and GluA2 subunits of AMPA receptors in hippocampus by enhancing activation of the IL-1 receptor: underlying mechanisms
Authors: L Taoro-Gonz, YM Arenas, A Cabrera-Pa, V Felipo
J Neuroinflammation, 2018-02-08;15(1):36.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot -
Anti-Inflammatory and Antinociceptive Effects of Ethyl Acetate Fraction of an Edible Red Macroalgae Sarcodia ceylanica
Authors: CC Shih, HR Hwang, CI Chang, HM Su, PC Chen, HM Kuo, PJ Li, HD Wang, KH Tsui, YC Lin, SY Huang, ZH Wen
Int J Mol Sci, 2017-11-17;18(11):.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
Microglia-derived IL-1? promotes chemokine expression by M�ller cells and RPE in focal retinal degeneration
Authors: R Natoli, N Fernando, M Madigan, JA Chu-Tan, K Valter, J Provis, M Rutar
Mol Neurodegener, 2017-04-24;12(1):31.
Species: Rat
Sample Types: In Vivo
Applications: Neutralization -
Inhibition of phagocytosis and pyroptosis of macrophages promotes Bartonella invasion into the bloodstream through lymphatic circulation
Authors: Congli Yuan
J. Infect. Dis., 2017-01-15;0(0):.
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot -
Neuroinflammation increases GABAergic tone and impairs cognitive and motor function in hyperammonemia by increasing GAT-3 membrane expression. Reversal by sulforaphane by promoting M2 polarization of microglia
Authors: V Hernandez-, A Cabrera-Pa, L Taoro-Gonz, A Gonzalez-U, A Agusti, T Balzano, M Llansola, V Felipo
J Neuroinflammation, 2016-04-18;13(1):83.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot -
Spatiotemporal Cadence of Macrophage Polarisation in a Model of Light-Induced Retinal Degeneration.
Authors: Jiao H, Natoli R, Valter K, Provis J, Rutar M
PLoS ONE, 2015-12-02;10(12):e0143952.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC-Fr -
Adenosine A2AR blockade prevents neuroinflammation-induced death of retinal ganglion cells caused by elevated pressure.
Authors: Madeira M, Elvas F, Boia R, Goncalves F, Cunha R, Ambrosio A, Santiago A
J Neuroinflammation, 2015-06-10;12(0):115.
Species: Rat
Sample Types: Tissue Culture Supernates
Applications: Neutralization -
The spinal anti-inflammatory mechanism of motor cortex stimulation: cause of success and refractoriness in neuropathic pain?
Authors: Silva G, Lopes P, Fonoff E, Pagano R
J Neuroinflammation, 2015-01-20;12(0):10.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
Spinal cord injury induces a long-lasting upregulation of interleukin-1beta in astrocytes around the central canal.
Authors: Paniagua-Torija B, Arevalo-Martin A, Molina-Holgado E, Molina-Holgado F, Garcia-Ovejero D
Neuroscience, 2014-10-19;284(0):283-9.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
Prolonged neuroinflammation after lipopolysaccharide exposure in aged rats.
Authors: Fu H, Yang T, Xiao W, Fan L, Wu Y, Terrando N, Wang T
PLoS ONE, 2014-08-29;9(8):e106331.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC-Fr -
Microglia enhance neurogenesis and oligodendrogenesis in the early postnatal subventricular zone.
Authors: Shigemoto-Mogami Y, Hoshikawa K, Goldman J, Sekino Y, Sato K
J Neurosci, 2014-02-05;34(6):2231-43.
Species: Rat
Sample Types: Whole Cells
Applications: Neutralization -
Inflammatory mediators induced by amyloid-beta in the retina and RPE in vivo: implications for inflammasome activation in age-related macular degeneration.
Authors: Liu R, Gao J, Cao S, Sandhu N, Cui J, Chou C, Fang E, Matsubara J
Invest Ophthalmol Vis Sci, 2013-03-01;54(3):2225-37.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC-P -
Keratinocyte expression of inflammatory mediators plays a crucial role in substance P-induced acute and chronic pain.
J Neuroinflammation, 2012-07-23;9(0):181.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC-Fr -
Microglial cells contribute to endogenous brain defenses after acute neonatal focal stroke.
Authors: Faustino JV, Wang X, Johnson CE
J. Neurosci., 2011-09-07;31(36):12992-3001.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
Unmasking of LPA1 receptor-mediated migration response to lysophosphatidic acid by interleukin-1beta-induced attenuation of Rho signaling pathways in rat astrocytes.
Authors: Sato K, Horiuchi Y, Jin Y, Malchinkhuu E, Komachi M, Kondo T, Okajima F
J. Neurochem., 2011-02-09;117(1):164-74.
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot -
Galectin-1 attenuates astrogliosis-associated injuries and improves recovery of rats following focal cerebral ischemia.
Authors: Qu WS, Wang YH, Ma JF, Tian DS, Zhang Q, Pan DJ, Yu ZY, Xie MJ, Wang JP, Wang W
J. Neurochem., 2010-12-02;116(2):217-26.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC-Fr -
Peripheral osmotic stimulation inhibits the brain's innate immune response to microdialysis of acidic perfusion fluid adjacent to supraoptic nucleus.
Authors: Summy-Long JY, Hu S
Am. J. Physiol. Regul. Integr. Comp. Physiol., 2009-09-16;297(5):R1532-45.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
Spinal leptin contributes to the pathogenesis of neuropathic pain in rodents.
Authors: Lim G, Wang S, Zhang Y, Tian Y, Mao J
J. Clin. Invest., 2009-01-12;119(2):295-304.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot -
Rat pneumonia and soft-tissue infection models for the study of Acinetobacter baumannii biology.
Authors: Russo TA, Beanan JM, Olson R, MacDonald U, Luke NR, Gill SR, Campagnari AA
Infect. Immun., 2008-06-09;76(8):3577-86.
Species: Rat
Sample Types: BALF
Applications: ELISA Development -
Systemic immune challenge activates an intrinsically regulated local inflammatory circuit in the adrenal gland.
Authors: Engstrom L, Rosen K, Angel A, Fyrberg A, Mackerlova L, Konsman JP, Engblom D, Blomqvist A
Endocrinology, 2008-01-03;149(4):1436-50.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC-Fr -
IL-1beta promotes neurite outgrowth by deactivating RhoA via p38 MAPK pathway.
Authors: Temporin K, Tanaka H, Kuroda Y, Okada K, Yachi K, Moritomo H, Murase T, Yoshikawa H
Biochem. Biophys. Res. Commun., 2007-11-08;365(2):375-80.
Species: Rat
Sample Types: Whole Cells
Applications: ICC -
Wnt-induced secreted protein-1 is a prohypertrophic and profibrotic growth factor.
Authors: Colston JT, de la Rosa SD, Koehler M, Gonzales K, Mestril R, Freeman GL, Bailey SR, Chandrasekar B
Am. J. Physiol. Heart Circ. Physiol., 2007-07-06;293(3):H1839-46.
Species: Rat
Sample Types: Whole Cells
Applications: Neutralization -
In vivo modulation of LPS-induced alterations in brain and peripheral cytokines and HPA axis activity by cannabinoids.
Authors: Roche M, Diamond M, Finn DP
J. Neuroimmunol., 2006-09-29;181(1):57-67.
Species: Rat
Sample Types: Plasma
Applications: ELISA Development -
Identification of astrocyte-expressed factors that modulate neural stem/progenitor cell differentiation.
Authors: Barkho BZ, Song H, Aimone JB
Stem Cells Dev., 2006-06-01;15(3):407-21.
Species: Rat
Sample Types: Whole Cells
Applications: Neutralization -
Single subcutaneous administration of chorionic gonadotropin to rats induces a rapid and transient increase in testicular expression of pro-inflammatory cytokines.
Authors: Assmus M, Svechnikov K, von Euler M, Setchell B, Sultana T, Zetterstrom C, Holst M, Soder O
Pediatr. Res., 2005-04-21;57(6):896-901.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
Ibuprofen protects ischemia-induced neuronal injury via up-regulating interleukin-1 receptor antagonist expression.
Authors: Park EM, Cho BP, Volpe BT, Cruz MO, Joh TH, Cho S
Neuroscience, 2005-01-01;132(3):625-31.
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot -
Differential cytokine response in interstitial fluid in skin and serum during experimental inflammation in rats.
Authors: Nedrebo T, Reed RK, Jonsson R, Berg A, Wiig H
J. Physiol. (Lond.), 2004-01-14;556(0):193-202.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC-Fr -
A dual role for apolipoprotein e in neuroinflammation: anti- and pro-inflammatory activity.
Authors: Guo L, LaDu MJ, Van Eldik LJ
J. Mol. Neurosci., 2004-01-01;23(3):205-12.
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot -
Glucocorticoids play a fundamental role in protecting the brain during innate immune response.
Authors: Nadeau S, Rivest S
J. Neurosci., 2003-07-02;23(13):5536-44.
Species: Rat
Sample Types: In Vivo
Applications: Neutralization
FAQs
No product specific FAQs exist for this product, however you may
View all Antibody FAQsReviews for Rat IL-1 beta /IL-1F2 Antibody
Average Rating: 5 (Based on 1 Review)
Have you used Rat IL-1 beta /IL-1F2 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Filter by:
This polyclonal antibody was used as both a capture and detection (the biotinylated version of this antibody, BAF501, was used as the detection) in a sandwich ELISA. Rat IL-1b was successful measured in serum samples.