Rat MCAM/CD146 APC-conjugated Antibody

Catalog #: FAB3250A Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB3250A-100UG

Detection of MCAM/CD146 in PC‑12 Rat Cell Line by Flow Cytometry.

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All MCAM/CD146 Antibodies

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Rat MCAM/CD146 APC-conjugated Antibody Summary

Species Reactivity

Rat

Specificity

Detects rat MCAM/CD146 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) MCAM, rhBCAM, or rhALCAM is observed.

Source

Monoclonal Mouse IgG_2A Clone # 404722

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat MCAM/CD146
Arg19-Lys560
Accession # Q9EPF2

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25-1 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of MCAM/CD146 in PC‑12 Rat Cell Line by Flow Cytometry. PC-12 rat adrenal pheochromocytoma cell line was stained with Mouse Anti-Rat MCAM/CD146 APC-conjugated Monoclonal Antibody (Catalog # FAB3250A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: MCAM/CD146

Melanomia Cell Adhesion Molecule (MCAM), also known as CD146 and MUC18, is a putative Ig-superfamily adhesion molecule that is expressed on endothelial cells and a variety of tumor cells. MCAM is associated with tumor progression and metastasis and may be involved in embryonic neural development. An alternately spliced isoform of rat MCAM has a 40 residue deletion in the cytoplasmic domain. Within the extracellular region, rat MCAM shares 72% and 90% amino acid sequence identity with human and mouse MCAM, respectively.

Long Name

Melanoma Cell Adhesion Molecule

Entrez Gene IDs

4162 (Human); 84004 (Mouse); 78967 (Rat)

Alternate Names

CD146 antigen; CD146; cell surface glycoprotein MUC18; Cell surface glycoprotein P1H12; L-Gicerin; MCAM; melanoma adhesion molecule; melanoma cell adhesion moleculeS-endo 1 endothelial-associated antigen; Melanoma-associated antigen A32; Melanoma-associated antigen MUC18; MUC18; MUC18Gicerin