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Rat TNF-alpha Antibody

Catalog #: MAB510
10 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB510-SP
MAB510-500
MAB510-100
Cytotoxicity Induced by TNF‑ alpha and Neutralization by Rat TNF‑ alpha Antibody.
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Product Details
Citations (10)
FAQs
Supplemental Products
Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat TNF-alpha Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat TNF-alpha in ELISAs and Western blots. In ELISAs, this antibody shows less than 3% cross-reactivity with recombinant mouse (rm) TNF‑ alpha and less than 0.2% cross-reactivity with rhTNF‑ alpha, rpTNF‑ alpha, and rhTNF‑ beta. In Western blots, this antibody shows 100% cross-reactivity with rmTNF‑ alpha  and no cross-reactivity with rfeTNF‑ alpha.
Source
Monoclonal Mouse IgG1 Clone # 45418
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant rat TNF-alpha
Accession # P16599
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Purity
Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Rat TNF‑ alpha (Catalog # 510-RT)

Rat TNF-alpha Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Rat TNF‑ alpha Biotinylated Antibody (Catalog # BAF510)

Standard: Recombinant Rat TNF-alpha Protein (Catalog # 510-RT)

Neutralization
Measured by its ability to neutralize TNF‑ alpha -induced cytotoxicity in the L‑929 mouse fibroblast cell line. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The Neutralization Dose (ND50) is typically 10-40 µg/mL in the presence of 0.025 ng/mL Recombinant Rat TNF‑ alpha.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cytotoxicity Induced by TNF‑ alpha and Neutralization by Rat TNF‑ alpha Antibody. View Larger

Cytotoxicity Induced by TNF‑ alpha and Neutralization by Rat TNF‑ alpha Antibody. Recombinant Rat TNF‑a (Catalog # 510-RT) induces cytotoxicity in the the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Cytotoxicity elicited by Recombinant Rat TNF‑a (0.025 ng/mL) is neutralized (green line) by increasing concentrations of Rat TNF‑a Monoclonal Antibody (Catalog # MAB510). The ND50 is typically 10-40 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TNF-alpha

Tumor Necrosis Factor Alpha (TNF-alpha ) also known as Cachectin, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. TNF-alpha is produced by a wide variety of immune and epithelial cell types (1, 2). Rat TNF-alpha consisits of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 179 aa extracellular domain (ECD) (3). Within the ECD, rat TNF-alpha shares 94% aa sequence identity with mouse and 69-76% with bovine, canine, cotton rat, equine, feline, human, porcine, and rhesus macaque TNF-alpha. The 26 kDa type 2 transmembrane protein is assembled intracellularly to form a noncovalently linked homotrimer (4). Ligation of this complex induces reverse signaling that promotes lymphocyte co-stimulation but diminishes monocyte responsiveness (5). Cleavage of membrane bound TNF-alpha by TACE/ADAM17 releases a 55 kDa soluble trimeric form of TNF-alpha (6, 7). TNF-alpha trimers bind the ubiquitous TNF RI and the hematopoietic cell-restricted TNF RII, both of which are also expressed as homotrimers (1, 8). TNF-alpha regulates lymphoid tissue development through control of apoptosis (2). It also promotes inflammatory responses by inducing the activation of vascular endothelial cells and macrophages (2). TNF-alpha is a key cytokine in the development of several inflammatory disorders (9). It contributes to the development of type 2 diabetes through its effects on insulin resistance and fatty acid metabolism (10, 11).

References
  1. Idriss, H.T. and J.H. Naismith (2000) Microsc. Res. Tech. 50:184.
  2. Hehlgans, T. and K. Pfeffer (2005) Immunology 115:1.
  3. Estler, H.C. et al. (1992) Biol. Chem. Hoppe-Seyler 373:271.
  4. Tang, P. et al. (1996) Biochemistry 35:8216.
  5. Eissner G. et al. (2004) Cytokine Growth Factor Rev. 15:353.
  6. Black, R.A. et al. (1997) Nature 385:729.
  7. Moss, M.L. et al. (1997) Nature 385:733.
  8. Loetscher, H. et al. (1991) J. Biol. Chem. 266:18324.
  9. Clark, I.A. (2007) Cytokine Growth Factor Rev. 18:335.
  10. Romanatto, T. et al. (2007) Peptides 28:1050.
  11. Hector, J. et al. (2007) Horm. Metab. Res. 39:250.
Long Name
Tumor Necrosis Factor alpha
Entrez Gene IDs
7124 (Human); 21926 (Mouse); 24835 (Rat); 397086 (Porcine); 280943 (Bovine); 403922 (Canine); 102139631 (Cynomolgus Monkey); 100033834 (Equine); 493755 (Feline); 100009088 (Rabbit)
Alternate Names
APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; TNFA; TNF-A; TNFalpha; TNF-alpha; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFG1F; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha

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Citations for Rat TNF-alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Interleukin-4 and Interleukin-13 Exacerbate Neurotoxicity of Prothrombin Kringle-2 in Cortex In Vivo via Oxidative Stress
    Authors: JY Jeong, YC Chung, BK Jin
    Int J Mol Sci, 2019-04-19;20(8):.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  2. Ulva lactuca polysaccharides prevent Wistar rat breast carcinogenesis through the augmentation of apoptosis, enhancement of antioxidant defense system, and suppression of inflammation
    Authors: GF Abd-Ellate, OM Ahmed, ES Abdel-Rehe, AZ Abdel-Hami
    Breast Cancer (Dove Med Press), 2017-02-27;9(0):67-83.
    Species: Rat
    Sample Types: Serum
    Applications: ELISA Development (Capture)
  3. Transport patterns of anti-TNF-? in burn wounds: Therapeutic implications of hyaluronic acid conjugation
    Authors: Newell R Washburn
    Biomaterials, 2016-11-04;114(0):10-22.
    Species: Rat
    Sample Types: Complex Sample Type, In Vivo
    Applications: Binding Assay, IHC, In Vivo
  4. Beneficial Effect of Moderate Exercise in Kidney of Rat after Chronic Consumption of Cola Drinks
    Authors: G Cao, J González, A Müller, G Ottaviano, G Ambrosio, JE Toblli, J Milei
    PLoS ONE, 2016-03-31;11(3):e0152461.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-P
  5. Urban traffic-derived nanoparticulate matter reduces neurite outgrowth via TNFalpha in vitro.
    Authors: Cheng H, Davis D, Hasheminassab S, Sioutas C, Morgan T, Finch C
    J Neuroinflammation, 2016-01-26;13(1):19.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Neutralization
  6. Neuronal synapse formation induced by microglia and interleukin 10.
    Authors: Lim S, Park E, You B, Jung Y, Park A, Park S, Lee J
    PLoS ONE, 2013-11-22;8(11):e81218.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Neutralization
  7. Dose-dependent changes in neuroinflammatory and arachidonic acid cascade markers with synaptic marker loss in rat lipopolysaccharide infusion model of neuroinflammation.
    BMC Neurosci, 2012-05-23;13(0):50.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  8. Rat pneumonia and soft-tissue infection models for the study of Acinetobacter baumannii biology.
    Authors: Russo TA, Beanan JM, Olson R, MacDonald U, Luke NR, Gill SR, Campagnari AA
    Infect. Immun., 2008-06-09;76(8):3577-86.
    Species: Rat
    Sample Types: BALF
    Applications: ELISA Development
  9. In vivo modulation of LPS-induced alterations in brain and peripheral cytokines and HPA axis activity by cannabinoids.
    Authors: Roche M, Diamond M, Finn DP
    J. Neuroimmunol., 2006-09-29;181(1):57-67.
    Species: Rat
    Sample Types: Plasma
    Applications: ELISA Development
  10. Oxidative mitochondrial DNA damage and deletion in hepatocytes of rejecting liver allografts in rats: role of TNF-alpha.
    Authors: Nagakawa Y, Williams GM, Zheng Q, Tsuchida A, Aoki T, Montgomery RA, Klein AS, Sun Z
    Hepatology, 2005-07-01;42(1):208-15.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC

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Rat TNF-alpha Antibody
By Anonymous on 10/18/2021
Application: WB Sample Tested: Serum Species: Rat
Western Blot Rat TNF-alpha Antibody MAB510
Rat TNF-alpha Antibody
By Anonymous on 11/08/2017
Application: ELISA Sample Tested: Serum and Plasma Species: Rat

An ELISA was build targeting rat TNFa using this antibody as a capture and BAF510 as the detection. The assay was used to measure TNFa in rat serum and plasma samples.

ELISA Rat TNF-alpha Antibody MAB510