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Rat TrkA Antibody

Catalog #: AF1056
26 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1056
AF1056-SP
Detection of Rat TrkA by Western Blot.
2 Images
Product Details
Citations (26)
FAQs
Supplemental Products
Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Rat TrkA Antibody Summary

Species Reactivity
Rat
Specificity
Detects rat TrkA in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human TrkA and less than 1% cross-reactivity with recombinant mouse (rm) TrkB and rmTrkC is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat TrkA
Ala33-Pro418
Accession # P35739
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated
Purity
Immunogen affinity purified

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Rat TrkA antibody by Western Blot. View Larger

Detection of Rat TrkA by Western Blot. Western blot shows lysate of rat striatum tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Rat TrkA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1056) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TrkA at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry TrkA antibody in Rat Dorsal Root Ganglion by Immunohistochemistry (IHC-Fr). View Larger

TrkA in Rat Dorsal Root Ganglion. TrkA was detected in perfusion fixed frozen sections of rat dorsal root ganglion using 15 µg/mL Rat TrkA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1056) overnight at 4 °C. Tissue was stained (red). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TrkA

TrkA, the product of the proto-oncogene trk, is a member of the neurotrophic tyrosine kinase receptor family that has three members. TrkA, TrkB, and TrkC preferentially bind NGF, NT-4, and BDNF and NT-3, respectively. All Trk family proteins share a conserved complex subdomain organization consisting of a signal peptide, two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, as well as an intracellular region that contains the tyrosine kinase domain. Two distinct rat TrkA isoforms (TrkA-I and Trk-A-II) that differ by a 6-amino acid insertion in their extracellular domain have been identified. The longer TrkA isoform is the only isoform expressed within neuronal tissues whereas the shorter TrkA-I is expressed mainly in non-neuronal tissues. NGF binds to TrkA with low affinity and activates its cytoplasmic kinase, initiating a signaling cascade that mediates neuronal survival and differentiation. Higher affinity binding of NGF requires the co-expression of TrkA with the p75 NGF receptor (NGF R), a member of the tumor necrosis factor receptor superfamily. NGF R binds all neurotrophins with low affinity and modulates Trk activity as well as alters the specificity of Trk receptors for their ligands. NGF R can also mediate cell death when expressed independent of Trk.

References
  1. Esposito, D. et al. (2001) J. Biol. Chem. 276:32687.
  2. Sofroniew, M.V. et al. (2001) Annu. Rev. Neurosci. 24:1217.
Long Name
Neurotrophic Tyrosine Kinase Receptor A
Entrez Gene IDs
4914 (Human); 18211 (Mouse); 59109 (Rat)
Alternate Names
DKFZp781I14186; EC 2.7.10; EC 2.7.10.1; MTChigh affinity nerve growth factor receptor; Neurotrophic tyrosine kinase receptor type 1; neurotrophic tyrosine kinase, receptor, type 1; NTRK1; NTRK-1; p140-TrkA; TRK1-transforming tyrosine kinase protein; TrkA; Trk-A; TRKAOncogene TRK; TRKTRK1; tyrosine kinase receptor A

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Citations for Rat TrkA Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

26 Citations: Showing 1 - 10
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  1. Titin is a nucleolar protein in neurons
    Authors: Cameron, B;Torres-Hernandez, L;Montague, VL;Lewis, KA;Smith, H;Fox, J;Guo, X;Kalb, RG;George, L;
    Research square
    Species: Murine polyomavirus strain A3
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  2. c-Maf-positive spinal cord neurons are critical elements of a dorsal horn circuit for mechanical hypersensitivity in neuropathy
    Authors: N Frezel, M Ranucci, E Foster, H Wende, P Pelczar, R Mendes, RP Ganley, K Werynska, S d'Aquin, C Beccarini, C Birchmeier, HU Zeilhofer, H Wildner
    Cell Reports, 2023-03-21;42(4):112295.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. FABP5 deletion in nociceptors augments endocannabinoid signaling and suppresses TRPV1 sensitization and inflammatory pain
    Authors: DM Bogdan, K Studholme, A DiBua, C Gordon, MP Kanjiya, M Yu, M Puopolo, M Kaczocha
    Oncogene, 2022-06-02;12(1):9241.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  4. Neuronal pentraxin 2 is required for facilitating excitatory synaptic inputs onto spinal neurons involved in pruriceptive transmission in a model of chronic itch
    Authors: K Kanehisa, K Koga, S Maejima, Y Shiraishi, K Asai, M Shiratori-, MF Xiao, H Sakamoto, PF Worley, M Tsuda
    Nature Communications, 2022-05-02;13(1):2367.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Piezo2 Knockdown Inhibits Noxious Mechanical Stimulation and NGF-Induced Sensitization in A-Delta Bone Afferent Neurons
    Authors: S Nencini, M Morgan, J Thai, AI Jobling, SB Mazzone, JJ Ivanusic
    Frontiers in Physiology, 2021-07-15;12(0):644929.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  6. Role of transient receptor potential vanilloid subtype 2 in lower oesophageal sphincter in rat acid reflux oesophagitis
    Authors: K Matsumoto, M Suenaga, Y Mizutani, K Matsui, A Yoshida, T Nakamoto, S Kato
    Journal of pharmacological sciences, 2021-04-07;146(3):125-135.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  7. Loss of Prdm12 during development, but not in mature nociceptors, causes defects in pain sensation
    Authors: MA Landy, M Goyal, KM Casey, C Liu, HC Lai
    Cell Reports, 2021-03-30;34(13):108913.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. Phox2a Defines a Developmental Origin of the Anterolateral System in Mice and Humans
    Authors: RB Roome, FB Bourojeni, B Mona, S Rastegar-P, R Blain, A Dumouchel, C Salesse, WS Thompson, M Brookbank, Y Gitton, L Tessarollo, M Goulding, JE Johnson, M Kmita, A Chédotal, A Kania
    Cell Rep, 2020-11-24;33(8):108425.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  9. Single cell RNA sequencing identifies early diversity of sensory neurons forming via bi-potential intermediates
    Authors: L Faure, Y Wang, ME Kastriti, P Fontanet, KKY Cheung, C Petitpré, H Wu, LL Sun, K Runge, L Croci, MA Landy, HC Lai, GG Consalez, A de Chevign, F Lallemend, I Adameyko, S Hadjab
    Nat Commun, 2020-08-21;11(1):4175.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. Neuronal aldosterone elicits a distinct genomic response in pain signaling molecules contributing to inflammatory pain
    Authors: M Shaqura, L Li, DM Mohamed, X Li, S Treskatsch, C Buhrmann, M Shakibaei, A Beyer, SA Mousa, M Schäfer
    J Neuroinflammation, 2020-06-12;17(1):183.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  11. ASIC3 inhibition modulates inflammation-induced changes in the activity and sensitivity of A&delta and C fiber sensory neurons that innervate bone
    Authors: M Morgan, J Thai, P Trinh, M Habib, KN Effendi, JJ Ivanusic
    Molecular pain, 2020-01-01;16(0):1744806920975.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  12. A cell fitness selection model for neuronal survival during development
    Authors: Y Wang, H Wu, P Fontanet, S Codeluppi, N Akkuratova, C Petitpré, Y Xue-Franzé, K Niederreit, A Sharma, F Da Silva, G Comai, G Agirman, D Palumberi, S Linnarsson, I Adameyko, A Moqrich, A Schedl, G La Manno, S Hadjab, F Lallemend
    Nat Commun, 2019-09-12;10(1):4137.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  13. Panicle-Shaped Sympathetic Architecture in the Spleen Parenchyma Modulates Antibacterial Innate Immunity
    Authors: X Ding, H Wang, X Qian, X Han, L Yang, Y Cao, Q Wang, J Yang
    Cell Rep, 2019-06-25;27(13):3799-3807.e3.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  14. Notch signalling defines dorsal root ganglia neuroglial fate choice during early neural crest cell migration
    Authors: S Wiszniak, Q Schwarz
    BMC Neurosci, 2019-04-29;20(1):21.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-F
  15. PRDM12 Is Required for Initiation of the Nociceptive Neuron Lineage during Neurogenesis
    Authors: L Bartesaghi, Y Wang, P Fontanet, S Wanderoy, F Berger, H Wu, N Akkuratova, F Bouçanova, JJ Médard, C Petitpré, MA Landy, MD Zhang, P Harrer, C Stendel, R Stucka, M Dusl, ME Kastriti, L Croci, HC Lai, GG Consalez, A Pattyn, P Ernfors, J Senderek, I Adameyko, F Lallemend, S Hadjab, R Chrast
    Cell Rep, 2019-03-26;26(13):3484-3492.e4.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  16. Prostaglandin E2 mediates sensory nerve regulation of bone homeostasis
    Authors: H Chen, B Hu, X Lv, S Zhu, G Zhen, M Wan, A Jain, B Gao, Y Chai, M Yang, X Wang, R Deng, L Wang, Y Cao, S Ni, S Liu, W Yuan, H Chen, X Dong, Y Guan, H Yang, X Cao
    Nat Commun, 2019-01-14;10(1):181.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  17. Differential modulation of voltage-gated sodium channels by nerve growth factor in three major subsets of TrkA-expressing nociceptors
    Authors: I Schaefer, V Prato, A Arcourt, FJ Taberner, SG Lechner
    Mol Pain, 2018-11-02;14(0):1744806918814.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  18. Optogenetic Activation of Non-Nociceptive A? Fibers Induces Neuropathic Pain-Like Sensory and Emotional Behaviors after Nerve Injury in Rats
    Authors: R Tashima, K Koga, M Sekine, K Kanehisa, Y Kohro, K Tominaga, K Matsushita, H Tozaki-Sai, Y Fukazawa, K Inoue, H Yawo, H Furue, M Tsuda
    eNeuro, 2018-02-15;5(1):.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  19. The Changing Sensory and Sympathetic Innervation of the Young, Adult and Aging Mouse Femur
    Authors: SR Chartier, SAT Mitchell, LA Majuta, PW Mantyh
    Neuroscience, 2018-02-10;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC
  20. [EXPRESS] Immunohistochemical localization of NGF, TrkA and p75 in the bone and articular cartilage of the mouse femur
    Authors: SR Chartier, SA Mitchell, LA Majuta, P Mantyth
    Mol Pain, 2017-11-22;0(0):1744806917745.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  21. Development of a pharmacodynamic biomarker to measure target engagement from inhibition of the NGF-TrkA pathway
    Authors: EA Price, A Krasowska-, KK Nanda, SJ Stachel, DA Henze
    J. Neurosci. Methods, 2017-03-07;282(0):34-42.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: ELISA Development (Capture)
  22. Unbiased classification of sensory neuron types by large-scale single-cell RNA sequencing.
    Authors: Usoskin D, Furlan A, Islam S, Abdo H, Lonnerberg P, Lou D, Hjerling-Leffler J, Haeggstrom J, Kharchenko O, Kharchenko P, Linnarsson S, Ernfors P
    Nat Neurosci, 2014-11-24;18(1):145-53.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  23. TREK2 expressed selectively in IB4-binding C-fiber nociceptors hyperpolarizes their membrane potentials and limits spontaneous pain.
    Authors: Acosta C, Djouhri L, Watkins R, Berry C, Bromage K, Lawson S
    J Neurosci, 2014-01-22;34(4):1494-509.
    Species: Rat
    Sample Types: Whole Cells
    Applications: IHC
  24. Temporal mismatch between pain behaviour, skin Nerve Growth factor and intra-epidermal nerve fibre density in trigeminal neuropathic pain.
    Authors: Evans L, Loescher A, Boissonade F, Whawell S, Robinson P, Andrew D
    BMC Neurosci, 2014-01-01;15(0):1.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  25. An intracellular domain fragment of the p75 neurotrophin receptor (p75(NTR)) enhances tropomyosin receptor kinase A (TrkA) receptor function.
    Authors: Matusica, Dusan, Skeldal, Sune, Sykes, Alex M, Palstra, Nickless, Sharma, Aanchal, Coulson, Elizabet
    J Biol Chem, 2013-03-07;288(16):11144-54.
    Species: Human, Rat
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  26. Electrical stimulation of sympathetic neurons induces autocrine/paracrine effects of NGF mediated by TrkA.
    Authors: Saygili E, Schauerte P, Kuppers F, Heck L, Weis J, Weber C, Schwinger RH, Hoffmann R, Schroder JW, Marx N, Rana OR
    J. Mol. Cell. Cardiol., 2010-02-02;49(1):79-87.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Neutralization

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Rat TrkA Antibody
By Anonymous on 10/31/2020
Application: ICC/IF Sample Tested: Bone Extracts Species: Mouse
Immunocytochemistry/Immunofluorescence Rat TrkA Antibody AF1056
Rat TrkA Antibody
By Anonymous on 11/22/2019
Application: ICC/IF Sample Tested: E12 DRG neurons Species: Mouse

E12 mouse DRG neurons grown overnight with 10ng/ml NGF, then fixed in 4% PFA, blocked for 1 h with 5% BSA, and incubated overnight in 1% BSA containing AF1056 (1:300). A fluorophore-conjugated secondary antibody was used to visualise reactivity.

Immunocytochemistry/Immunofluorescence Rat TrkA Antibody AF1056