Recombinant Canine IL-2 (Cys147Ser) Protein

Name: Recombinant Canine IL-2 (Cys147Ser) Protein
Brand: R&D Systems
SKU: 1815-CL
Rating: 4 (1 reviews)

Catalog #: 1815-CL
2 Citations 1 Review Datasheet / COA / SDS

Carrier Free

Catalog #	Availability	Size / Price	Qty
1815-CL-020/CF

With Carrier

Catalog #	Availability	Size / Price	Qty
1815-CL-020

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Product Details

Citations (2)

FAQs

Reviews (1)

Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Canine IL-2 (Cys147Ser) Protein Summary

Product Specifications

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<0.1 EU per 1 μg of the protein by the LAL method.

Activity

Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 295. The ED₅₀ for this effect is 0.15‑0.8 ng/mL.

Source

E. coli-derived canine IL-2 protein
Ala21-Thr155 (Cys147Ser), with and without an N-terminal Met

Accession #

Q29416

N-terminal Sequence
Analysis

Met & Ala21

Predicted Molecular Mass

15.6 kDa

Product Datasheets

1815-CL (with carrier)

Product Datasheet

COA

SDS

1815-CL/CF (carrier free)

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1815-CL

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1815-CL/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

Background: IL-2

Interleukin 2 was initially identified as a T cell growth factor that is produced by T cells following activation by mitogens or antigens (1). Since then, it has been shown that IL-2 can also stimulate the growth and differentiation of B cells, natural killer (NK) cells, lymphocyte activated killer (LAK) cells, monocytes/macrophages, and oligodendrocytes (2). The biological activity of IL-2 is mediated by the binding to cell surface receptor complexes composed of three subunits designated as alpha, beta, and gamma subunits (3). IL-2 binds the alpha subunit with low affinity. The functional high affinity IL-2 receptor is a heterotrimeric complex of the alpha, beta, and gamma subunits. IL-2 binds with intermediate affinity to the complex containing the beta and gamma subunits, which is also capable of transducing IL-2 signals (4). In T cells, the beta and gamma subunits are shared with the IL-15 receptor complex (5). The gamma subunit of the IL-2 receptor complex has also been shown to be a subunit of the receptor complexes of IL-4, IL-7, and IL-9 (6). At the amino acid sequence level, canine IL-2 shares 90%, 86%, 85%, 76%, and 75% sequence similarities to feline, human, equine, mouse, and bovine IL-2, respectively (7).

References

Morgan, D.A. et al. (1976) Science 193:1007.
Smith, K.A. et al. (1988) Science 240:1169.
Taniguchi, T. and Y. Minami (1993) Cell 73:5.
Giri, J. et al. (1994) EMBO J. 13:2822.
Waldmann, T. et al. (1998) Int. Rev. Immunol. 16:205.
Nelson, B.H. and D.M. Willeford (1998) Adv. Immunol. 70:1.
Dunham, S.P. et al. (1995) DNA Seq. 5:177.

Long Name

Interleukin 2

Entrez Gene IDs

3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)

Alternate Names

Aldesleukin; IL2; IL-2; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; Proleukin; T cell growth factor; T-cell growth factor; TCGF

Related Research Areas

Citations for Recombinant Canine IL-2 (Cys147Ser) Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Role of NK-Like CD8+ T Cells during Asymptomatic Borrelia burgdorferi Infection
Authors: BM Scorza, KG Mahachi, AD Cox, AJ Toepp, D Pessoa-Per, P Tyrrell, J Buch, JA Foltz, D Lee, CA Petersen
Infection and Immunity, 2022-04-13;0(0):e0055521.
Species: Canine
Sample Types: Whole Cells
Applications: Bioassay
Safety of AAV factor IX peripheral transvenular gene delivery to muscle in hemophilia B dogs.
Authors: Haurigot V, Mingozzi F, Buchlis G
Mol. Ther., 2010-04-27;18(7):1318-29.
Species: Canine
Sample Types: Whole Cells
Applications: Bioassay