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Recombinant Cynomolgus Fc gamma RIIA/CD32a Protein, CF

Catalog #: 9240-CD Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
9240-CD-100
Recombinant Cynomolgus Fc gamma RIIA/CD32a Protein Bioactivity
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Cynomolgus Fc gamma RIIA/CD32a Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey Fc gamma  RIIA/CD32a is immobilized on a His Tag Antibody coated plate, it binds Biotinylated Human IgG. The concentration of Biotinylated Human IgG that produces 50% of the optimal binding response is approximately
0.75-3.75 μg/mL.
Source
Human embryonic kidney cell, HEK293-derived cynomolgus monkey Fc gamma RIIA/CD32a protein
Thr29-Ile211, with a C-terminal 6-His tag
Accession #
NP_001270598
N-terminal Sequence
Analysis
Thr29
Predicted Molecular Mass
21 kDa
SDS-PAGE
23-32 kDa, reducing conditions

Product Datasheets

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9240-CD

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9240-CD

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Cynomolgus Fc gamma RIIA/CD32a Protein Bioactivity View Larger

When Recombinant Cynomolgus Monkey Fc gamma RIIA/CD32a (Catalog # 9240-CD) is immobilized on a His Tag Antibody coated plate, Biotinylated Human IgG binds with an ED50 of 0.75-3.75 μg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Fc gamma RIIA/CD32a

Receptors for the Fc region of IgG (Fc gamma  Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, ADCC (antibody-dependent cellular toxicity), cytokine release, and B cell proliferation (1, 2). The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma  RI/CD64, Fc gamma  RII/CD32, and Fc gamma  RIII/CD16. CD32 proteins are encoded by three genes in human (Fc gamma RII A, B, and C), two in cynomolgus (Fc gamma RII A and B), and one in mouse (Fc gamma RIIB). Fc gamma RII A and C contain ITAM sequences, while Fc gamma RIIB contains an ITIM sequence in their cytoplasmic domains. The CD64 proteins are high affinity receptors (~10-8-10-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10-6-10-7 M) and only recognize IgG aggregates surrounding multivalent antigens (1, 3). Mature cynomolgus Fc gamma RIIA consists of a 183 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 23 aa transmembrane segment, and a 77 aa cytoplasmic domain. Within the ECD, cynomolgus Fc gamma RIIA shares 89% aa sequence identity with human Fc gamma RIIA. It binds cynomolgus and human IgG subclasses 1-4 as well as human Pentraxin-2, and C-Reactive Protein (4, 5). It delivers activating signals through its cytoplasmic ITAM sequence (6). Fc gamma RIIA signaling cooperates with Integrin alpha 2b beta 3 and the CD42b-GP1b-IX-V complex to promote platelet adhesion and activation (7, 8). Fc gamma RIIA is expressed on monocytes, monocytes, and platelets and additionally on cynomolgus granulocytes (1, 4).

References
  1. Qiao, J. et al. (2015) Immunol. Rev. 268:241.
  2. Ravetch, J. and S. Bolland (2001) Annu. Rev. Immunol. 19:275.
  3. Takai, T. (2002) Nature Rev. Immunol. 2:580.
  4. Warncke, M. et al. (2012) J. Immunol. 188:4405.
  5. Lu, J. et al. (2008) Nature 456:989.
  6. Rosenfeld, S.I. et al. (1985) J. Clin. Invest. 76:2317.
  7. Sullam, P.M. et al. (1998) J. Biol. Chem. 273:5331.
  8. Boylan, B. et al. (2008) Blood 112:2780.
Long Name
Fc gamma Receptor II A
Entrez Gene IDs
2212 (Human); 14130 (Mouse); 116591 (Rat); 102143568 (Cynomolgus Monkey)
Alternate Names
CD32 antigen; CD32a; CD32MGC23887; CDw32fc-gamma-RIIa; Fc fragment of IgG, low affinity IIa, receptor (CD32); Fc fragment of IgG, low affinity IIa, receptor for (CD32); Fc gamma RIIA; FCG2; Fc-gamma RII-a; Fc-gamma-RIIa; FcGR; FCGR2; FCGR2A; FCGR2A1; FcgRIIA; FCRIIA; fcRII-a; IGFR2MGC30032; IgG Fc receptor II-a; Immunoglobulin G Fc receptor II; low affinity immunoglobulin gamma Fc region receptor II-a

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